Separation,purification and antibacterial activity of total flavonoids from Catalpa root bark

Authors

SHAO Jinhua, Hunan University of Science and Technology, Engineering Technology Research Center on Ginkgo of Hunan, Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, Yongzhou, Hunan 425100, China
HE Fulin, Hunan University of Science and Technology, Engineering Technology Research Center on Ginkgo of Hunan, Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, Yongzhou, Hunan 425100, China
CHEN Xia, Hunan University of Science and Technology, Engineering Technology Research Center on Ginkgo of Hunan, Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, Yongzhou, Hunan 425100, China
XIE Yu, Hunan University of Science and Technology, Engineering Technology Research Center on Ginkgo of Hunan, Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, Yongzhou, Hunan 425100, China

Abstract

Taking the adsorption rate and desorption rate as the evaluation indexes, macroporous resin was adopted to optimize the separation and purification technology of total flavonoids from Catalpa root bark, and the antibacterial activity of the purified total flavonoids from Catalpa root bark was investigated. The results indicated that the optimal technological conditions for the separation and purification of total flavonoids from Catalpa root bark were as follows: application of NKA-II resin; mass concentration, flow rate and pH value of loading buffer of 0.538 mg/mL, 1.66 mL/min and 4.01, respectively; concentration of eluant ethyl alcohol of 70%; and elution flow rate of 2.5 mL/min. Under such conditions, the purity, adsorption rate and desorption rate of total flavonoids from Catalpa root bark were (77.43±0.23)%, (93.36±0.25)% and (95.51±0.17)%, respectively. Antibacterial activity indicated that total flavonoids from Catalpa root bark had extremely sensitive antibacterial activity to Escherichia coli, Bacillus subtilis, and Staphylococcus aureus; and high antibacterial sensitivity to penicillium and Saccharomyces cerevisiae. MIC values of S. aureus, Bacillus subtilis and E. coli, as well as S. cerevisiae and penicillium were (6.25±0.25) mg/mL, (12.50±0.36) mg/mL and (25.00±0.27) mg/mL, respectively.

Publication Date

2-28-2017

First Page

140

Last Page

144,220

DOI

10.13652/j.issn.1003-5788.2017.02.030

Recommended Citation

Jinhua, SHAO; Fulin, HE; Xia, CHEN; and Yu, XIE (2017) "Separation,purification and antibacterial activity of total flavonoids from Catalpa root bark," Food and Machinery: Vol. 33: Iss. 2, Article 30.
DOI: 10.13652/j.issn.1003-5788.2017.02.030
Available at: https://www.ifoodmm.cn/journal/vol33/iss2/30

References

[1] 新疆维吾尔自治区革命委员会卫生局. 新疆中草药[M]. 乌鲁木齐: 新疆人民出版社,1975: 318.
[2] 王奇志, 梁敬钰. 梓属植物化学成分研究进展[J]. 中草药, 2003, 34(27): 2-4.
[3] AN S J, PAE H O, OH G S, et al. Inhibition of TNF-ａ, IL-β, and IL-6 productions and NF-kB activation in lipopolysaccharide-activated RAW macrophages by catalposide,an iridoid glycoside isolated from Catalpa ovata G.Don (Bignoniaceae) [J]. Internetional Immunopharacology, 2002(2): 1 173-1 181.
[4] PAE H O, OH G S, CHOU B M, et al. Inhibitory effects of the sterm bark of Catalpa ovata G.Don. (Bignoniaceae) onthe productions of tumor necrosis factor-ａ and nitric oxide by the lipopolisaccharide-stimulated RAW macrophages[J]. Journal of Ethnopharmacology, 2003(88): 287-291.
[5] 陈龙, 景丹龙, 王军辉. 6个不同种源梓树叶片的总酚含量和抗氧化性能的比较[J]. 西北林学院学报, 2015, 30(6): 222-226.
[6] 邵金华, 李军艳, 刘欢, 等.梓树根皮有效成分的初步研究[J]. 湖南科技学院学报, 2014, 35(10): 46-49.
[7] 张静, 张晓鸣, 佟建明, 等. 金属络合法纯化银杏黄酮的研究[J]. 天然产物研究与开发, 2010, 22(5): 751-754, 756.
[8] 陈丛瑾, 屈丽娟, 陈东. 双水相萃取法分离纯化黄酮类化合物的研究进展[J]. 应用化工, 2010, 39(10): 1 587-1 590.
[9] 陈丛瑾. 柱色谱法分离纯化黄酮类化合物研究进展[J]. 西北药学杂志, 2011, 26(2): 150-153．
[10] CHEN Ming-lei, HU Wei, ZHANG Chao, et al. High performance liquid chromatography for the determination of flavonolids[J]. Journal of Chinese Pharmaceutical Sciences, 2011(20): 313-324.
[11] 许浮萍, 梁志家, 田娟娟. 应用膜分离结合醇沉法纯化大豆异黄酮[J]. 食品科学, 2009, 30(16): 78-82.
[12] 李升锋, 徐玉娟, 陈智毅, 等. 微乳薄层层析法分离桑叶中的黄酮类化合物[J]. 食品科学, 2008, 29(6): 87-89.
[13] 钟方丽, 王晓林, 王志敏, 等. 大孔吸附树脂纯化玉竹总黄酮工艺研究[J]. 食品与机械, 2013, 29(1): 131-134.
[14] 罗艳玲, 欧仕益. 大孔树脂在食品活性成分分离中的应用[J]. 食品与机械, 2005, 21(5): 81-83.
[15] 迟晓喆, 曹光群. 桑叶总黄酮的提取及其抑菌活性研究[J]. 林产化学与工业, 2012, 32(2): 163-166.
[16] 陈乃东, 周守标, 罗琦, 等. 不同提取剂对春花胡枝子黄酮含量及抑菌活性影响的研[J]. 中国卫生检验杂志, 2007, 17(2): 193-196.
[17] 李国章, 于华忠, 卜晓英, 等. 桑椹籽中黄酮的CO₂超临界流体萃取及抑菌作用研究[J]. 现代食品科技, 2006, 22(2): 86-88.
[18] 任萍, 王齐伟, 武彩露, 等. 大孔吸附树脂对罗布麻叶中总黄酮的纯化研究[J]. 离子交换与吸附, 2016, 32(2): 141-153
[19] 萨茹丽, 木其尔, 王翠芳, 等. 沙葱总黄酮提取工艺优化及其体外抗氧化、抗菌作用[J]. 食品科学, 2014, 35(24): 1-8.
[20] 张婷婷, 郭夏丽, 黄学勇, 等. 辛夷挥发油GC-MS分析及其抗氧化、抗菌活性[J]. 食品科学, 2016, 37(10): 144-150.
[21] 王国军, 唐辉, 张淑兰, 等. 大孔树脂纯化核桃隔膜总黄酮的工艺研究[J]. 中草药, 2013, 44(19): 2 688-2 692.
[22] 李淑珍, 李进, 杨志江, 等. 大孔树脂分离纯化黑果枸杞总黄酮的研究[J]. 食品科学, 2009, 30(1): 19-24.
[23] 赵玉芬, 李佩艳, 郑小林, 等. 大孔树脂对红薯叶总黄酮的吸附及解吸特性研究[J]. 生物技术进展, 2013, 6(3): 433-438.
[24] 陈丽娜, 吴琼, 高长城. 大孔树脂分离纯化沙棘籽及果皮渣黄酮的工艺研究[J]. 农业机械, 2012(6): 146-148.
[25] 董江涛, 李燕, 徐慧强, 等. 大孔树脂纯化柿叶总黄酮的工艺研究[J]. 湖南农业科学, 2010(11): 85-89.
[26] 李国旺, 苗志国, 赵恒章. 大黄的体外抑菌实验[J]. 光谱实验室, 2011, 28(1): 66-68.