Optimization oflow 3-MCPD wheat gluten protein hydrolysate by response surface methodology

Authors

GUO Qingya, Beijing High-tech Innovation Centre of Food Nutrition and Human Health, Beijing 100048, China; Laboratory of Molecular Sensory Science, Beijing Technology and Business University, Beijing 100048, China
SONG Huanlu, Beijing High-tech Innovation Centre of Food Nutrition and Human Health, Beijing 100048, China; Laboratory of Molecular Sensory Science, Beijing Technology and Business University, Beijing 100048, China
CHAI Dan, Wilmar (Shanghai) Biotechnology Research and Development Center Co., Ltd, Shanghai 200137, China
CHEN Ying, Wilmar (Shanghai) Biotechnology Research and Development Center Co., Ltd, Shanghai 200137, China
LUO Huanzhen, Beijing High-tech Innovation Centre of Food Nutrition and Human Health, Beijing 100048, China; Laboratory of Molecular Sensory Science, Beijing Technology and Business University, Beijing 100048, China
YANG Ping, Beijing High-tech Innovation Centre of Food Nutrition and Human Health, Beijing 100048, China; Laboratory of Molecular Sensory Science, Beijing Technology and Business University, Beijing 100048, China

Abstract

Given the high quality wheat gluten protein, the hydrolysis conditions including hydrochloric acid concentration, hydrolysis time and solid-liquid ratio were optimized with the degree of hydrolysis, umami amino acids (aspartic and glutamic acid) as indices using hydrochloric acid, and the processing technique was expatiated. Moreover, 3-monochloropropane-1,2-diol (3-MCPD) combining with acidolysis process was measured and sensory evaluation assessment team was organized to do the sensory evaluation of hydrolyzed vegetable protein under different conditions. Additionally, the optimum preparation was selected. Finally, on the basis of single factor experiment, the response surface of the 3 level design (Box-Behnken design) was used by degree of hydrolysis and glutamic acid content as indexes to optimize the hydrochloric acid concentration, hydrolysis time and solid-liquid ratio. The best acid hydrolysis condition included a hydrochloric acid concentration at 1.6 mol/L, a solid-liquid ratio at 14, hydrolyzed for 4 h. Furthermore, 3-MCPD was compared before and after using activated carbon, thus expecting to get high-quality and umami, safe and low content 3-MCPD content wheat gluten protein hydrolysate for industrial mass production.

Publication Date

2-28-2017

First Page

162

Last Page

168

DOI

10.13652/j.issn.1003-5788.2017.02.035

Recommended Citation

Qingya, GUO; Huanlu, SONG; Dan, CHAI; Ying, CHEN; Huanzhen, LUO; and Ping, YANG (2017) "Optimization oflow 3-MCPD wheat gluten protein hydrolysate by response surface methodology," Food and Machinery: Vol. 33: Iss. 2, Article 35.
DOI: 10.13652/j.issn.1003-5788.2017.02.035
Available at: https://www.ifoodmm.cn/journal/vol33/iss2/35

References

[1] 李小艳, 李高阳, 任国谱. 小麦面筋蛋白脱酰胺改性研究进展[J]. 食品工业科技, 2014, 35(8): 390-393.
[2] 赵谋明. 植物蛋白酶解改性研究进展: 小麦面筋蛋白与花生蛋白[J]. 中国食品添加剂, 2008(S1): 188-193.
[3] AULT A. The Monosodium Glutamate Story: The Commercial Production of MSG and Other Amino Acids[J]. Journal of Chemical Education, 2004, 81(3): 347-355.
[4] 黄镇洲. 水解植物蛋白(HVP)的生产工艺研究[J]. 中国调味品, 1995(3): 14-17.
[5] 彭睆睆. 利用小麦面筋蛋白制备呈味基料及呈味肽的分离鉴定[D]. 广州: 华南理工大学, 2014: 1.
[6] SCHLICHTHERLECERNY H, AMAD R. Analysis of Taste-Active Compounds in an Enzymatic Hydrolysate of Deamidated Wheat Gluten[J]. Journal of Agricultural & Food Chemistry, 2002, 50(6): 1 515-1 522.
[7] 崔洁. 小麦面筋蛋白酶解及膜分离特性研究[D]. 无锡: 江南大学, 2010: 1.
[8] 陆恒. 面筋蛋白质的营养价值及其利用[J]. 粮食与饲料工业, 1989(2): 57-59.
[9] 徐添. 谷朊粉挤压改性制备小麦组织蛋白的工艺和应用研究[D]. 合肥: 合肥工业大学, 2013: 36.
[10] 黄婵媛. 小麦面筋蛋白控制酶解制备呈味基料的研究[D]. 广州: 华南理工大学, 2011: 1.
[11] 王悦宏. 快速测定HVP和酱油中3-MCPD方法的研究与应用[D]. 长春: 东北师范大学, 2008: 4.
[12] 史亚静, 宋焕禄. 酸酶结合法制备大豆蛋白水解液的工艺探讨[J]. 食品科技, 2011, 36(4): 2-5.
[13] 陈均志, 李祥. 复合氨基酸调味液生产[J]. 中国调味品, 1991(3): 23-26.
[14] YAMAGUCHI S. The Synergistic Taste Effect of Monosodium Glutamate and Disodium 5′-Inosinate[J]. Journal of Food Science, 2006, 32(4): 473-478.
[15] FILER L J J, GARATTINI S, KARE M R, et al. Glutamic acid: advances in biochemistry and physiology[J]. The Quarterly Review of Biology, 1979, 5(11): 35-54.
[16] 丁志刚, 高红梅, 潘冬梅, 等. 复合酶法水解小麦面筋蛋白的研究[J]. 食品工业科技, 2014, 35(20): 91-195.
[17] ROMBOUTS I, LAMBERTS L, CELUS I, et al. Wheat gluten amino acid composition analysis by high-performance anion-exchange chromatography with integrated pulsed amperometric detection[J]. Journal of Chromatography A, 2009, 1 216(29): 5 557-5 562.
[18] MARTINEZ-ARELLANO I, FLORES M, TOLDR F. The ability of peptide extracts obtained at different dry cured hamripening stages to bind aroma compounds[J]. Food Chemistry, 2016, 196: 9-16.
[19] LIN Shin-yi, LO Yu-chang. Nonvolatile Taste Components and Functional Compounds of Commercial Soy Sauce Products[J]. Journal of Food Processing and Preservation, 2015, 39(6): 2 680-2 686.