Extraction process optimization and anticancer activity of maize yellow pigment

Authors

YU Hui, School of Life Science, Liaoning University, Shenyang, Liaoning 110036, China
LIU Meijia, School of Life Science, Liaoning University, Shenyang, Liaoning 110036, China
LIU Jianli, School of Life Science, Liaoning University, Shenyang, Liaoning 110036, China
CAO Xiangyu, School of Life Science, Liaoning University, Shenyang, Liaoning 110036, China

Abstract

Objective: To study the best preparation technology of maize yellow pigment by ultrasonic assisted with enzymatic method, and the inhibitory effect of them on the growth of human gastric cancer cells (BGC-823). Methods: The optimum conditions of maize yellow pigment were extracted by ultrasonic assisted with enzymatic method by single factor and response surface experiments. The inhibition of maize yellow pigment on BGC-823 cells was detected by morphological observation and MTT assay to evaluate the antitumor activity. Results: the maize yellow pigment best extraction condition of ultrasonic with enzymatic method is: enzyme activity 10 000 U/g, ultrasonic power 400 W, enzymolysis temperature 35 ℃, the maximum extraction yield was (72.64 ± 0.42) μg/g under the condition, the maize yellow pigment has a certain inhibitory effect on BGC-823 cell were studied by cell morphological observation and MTT test. Conclusion: The extraction of maize yellow pigment by ultrasonic assisted with enzymatic method is a kind of effective extraction method, the maize yellow pigment prepared has strong inhibitory effect on BGC-823 cells.

Publication Date

3-28-2017

First Page

139

Last Page

144

DOI

10.13652/j.issn.1003-5788.2017.03.030

Recommended Citation

Hui, YU; Meijia, LIU; Jianli, LIU; and Xiangyu, CAO (2017) "Extraction process optimization and anticancer activity of maize yellow pigment," Food and Machinery: Vol. 33: Iss. 3, Article 29.
DOI: 10.13652/j.issn.1003-5788.2017.03.030
Available at: https://www.ifoodmm.cn/journal/vol33/iss3/29

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