Optimization on microwave-two enzymes synergistic methods for dietary fiber extraction in lotus seed shell

Authors

CHEN Tiebi, Department of Biology and Chemistry, Hunan University of Science and Technology, Yongzhou, Hunan 425100, China; Hunan Provincial Engineering Research Center for Food Processing of Aquatic Biotic Resources, Changsha University of Science and Technology, Ch
JIN Na, Hunan Provincial Engineering Research Center for Food Processing of Aquatic Biotic Resources, Changsha University of Science and Technology, Changsha, Hunan 410114, China
WANG Jianhui, Hunan Provincial Engineering Research Center for Food Processing of Aquatic Biotic Resources, Changsha University of Science and Technology, Changsha, Hunan 410114, China
LIU Dongmin, Department of Biology and Chemistry, Hunan University of Science and Technology, Yongzhou, Hunan 425100, China; Hunan Provincial Engineering Research Center for Food Processing of Aquatic Biotic Resources, Changsha University of Science and Technology, Ch
FAN Min, Department of Biology and Chemistry, Hunan University of Science and Technology, Yongzhou, Hunan 425100, China
DENG Guosheng, Department of Biology and Chemistry, Hunan University of Science and Technology, Yongzhou, Hunan 425100, China
CHEN Yingyi, Department of Biology and Chemistry, Hunan University of Science and Technology, Yongzhou, Hunan 425100, China

Abstract

To investigate the effect of microwave-two enzymes synergistic extraction methods on dietary fiber yields in lotus shell, the sieving mesh number of the lotus seed shell, ratio of solid-liquid, papain and glucoamylase dosages and extraction time were set out to conduct the orthogonal tests based on the results of single factor experiments. The optimal extraction conditions were as followed: sieving mesh of 60, ratio of liquid-solid 201, papain dosage 3%, papain extraction time of 6×20 s (extracting 6 times, each time for 20 s with intervals of 2 min), dosage of glucoamylase 4%, and glucoamylase extraction time 8×20s (extracting 8 times, each time for 20 s with intervals of 2 min). Under the optimal conditions, the extraction rate of dietary fiber in lotus shell was 72.36%. Compared with enzyme extraction, microwave-two enzymes synergistic extraction can save 70% time, and greatly improve the extraction rate over 82.87% than two enzyme method.

Publication Date

3-28-2017

First Page

164

Last Page

167

DOI

10.13652/j.issn.1003-5788.2017.03.035

Recommended Citation

Tiebi, CHEN; Na, JIN; Jianhui, WANG; Dongmin, LIU; Min, FAN; Guosheng, DENG; and Yingyi, CHEN (2017) "Optimization on microwave-two enzymes synergistic methods for dietary fiber extraction in lotus seed shell," Food and Machinery: Vol. 33: Iss. 3, Article 34.
DOI: 10.13652/j.issn.1003-5788.2017.03.035
Available at: https://www.ifoodmm.cn/journal/vol33/iss3/34

References

［1］ 刘晓婷. 膳食纤维的开发及应用［J］. 中国食品与营养, 2004(9): 21-23.
［2］ 赵二劳, 王璐. 膳食纤维的保健功能及其制备研究进展［J］. 食品与机械, 2011, 27(3): 165-168.
［3］ 李建文, 杨月欣. 膳食纤维定义及分析方法研究进展［J］. 食品科学, 2007, 28(2): 350-355.
［4］ 吴非, 于胜男, 葛锡娟, 等. 菌质水溶性膳食纤维对小鼠肠蠕动与吸收的影响［J］. 食品与机械, 2015, 31(3): 8-10, 31.
［5］ 张艳, 何翠, 刘玉凌, 等. 超声波改性对方竹笋膳食纤维性能和结构的影响［J］. 食品与发酵工业, 2017, 43(1): 151-155.
［6］ 周德龙, 高建华, 杨祎静, 等. 莲子壳营养成分分析及类黄酮抗氧化活性研究［J］. 安徽农业科学, 2011, 39(7): 3 968-3 970.
［7］ 李焕霞, 任志, 王华, 等. 我国膳食纤维研究现状分析［J］. 中国食品添加剂, 2007(2): 161-164.
［8］ 胥晶, 张涛, 江波. 国内外膳食纤维的研究进展［J］. 食品工业科技, 2009, 30(6): 360-367.
［9］ 唐仕荣, 刘全德, 苗敬芝, 等. 超声波协同酶法提取牛蒡膳食纤维工艺［J］. 徐州工程学院学报, 2011, 26(1): 6-11.
［10］ 王建辉, 刘永乐, 李赤翎, 等. 丝兰皂甙的微波辅助提取工艺研究［J］. 食品科学, 2012, 33(2): 58-62.
［11］ 郑慧, 陈希平, 尤祯丹, 等. 蜂花粉可溶性膳食纤维酶法提取工艺优化及其理化分析［J］. 食品与机械, 2016, 32(12): 184-188.
［12］ 杨梦曦, 朱叶, 邓雪盈, 等. 复合酶法提取豆渣膳食纤维的研究［J］. 食品与机械, 2014, 30(4): 186-189.