Abstract
CFP-1 and CFP-2 were purified from Corbicula fluminea by DEAE-52 cellulose anion-exchange chromatography and SephadexTMG-100 gel-filfration chromatography. The molecule structure was analyzed by ultraviolet spectrum scanning and infraned spectrum. Ultraviolet spectrum showed that CFP-1 and CFP-2 contained little DNA and protein, and infraned spectrum showed that both of them were polysaccharides. Purity of CFP-1 and CFP-2 was further confirmed by using HPLC, they were homogenous with a molecular weight of 1 172 kD and 3 627 kD. The result showed that CFP-1 could significantly inhibit proliferation of HepG-2 than CFP-2. with the IC50 value of 0.24 mg/mL after 48 h treating. In addition, radical scavenging tests revealed that CFP-2 showed significant function of antioxidation activity.
Publication Date
4-28-2017
First Page
127
Last Page
132,138
DOI
10.13652/j.issn.1003-5788.2017.04.025
Recommended Citation
Qi, ZHAO; Li, ZHAO; Yuluan, YANG; Lili, CHEN; Meilan, YUAN; and Chunqing, BAI
(2017)
"Study onisolation, antioxidation and anti-tumor activites of polysaccharide in Corbicula fluminea,"
Food and Machinery: Vol. 33:
Iss.
4, Article 25.
DOI: 10.13652/j.issn.1003-5788.2017.04.025
Available at:
https://www.ifoodmm.cn/journal/vol33/iss4/25
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