•  
  •  
 

Abstract

To comprehensively develop and utilize Camellia resources, fractions of ethanol extract of Camellia semiserrata Chi. cake were separated and identified. Five purified compounds with purity of 99.2%, 95.4%, 97.5%, 99.1% and 97.4%, respectively, were achieved. By using affiliated molecular formula of generator by Agilent high-resolution mass spectrometry workstation Qualitative Analysis B.06.00, formula of MS fragmentation of molecular ions were calculated, combining with fragmentation patterns of compounds and physical and chemical properties, the compounds were identified as protocatechuic acid and four kinds of flavonoids.

Publication Date

4-28-2017

First Page

133

Last Page

138

DOI

10.13652/j.issn.1003-5788.2017.04.026

References

[1] BIGLARI F, ALKARKHI A F M, EASA A M. Antioxidant activity and phenolic content of various date palm (Phoenix dactylifera) fruits from Iran[J]. Food Chemistry, 2008, 107(4): 1 636-1 641.
[2] REHECHO S, HIDALGO O, GARCA-IIGUEZ De Cirano M, et al. Chemical composition, mineral content and antioxidant activity of Verbena officinalis L.[J]. LWT-Food Science and Technology, 2011, 44(4): 875-882.
[3] SHUI G, LEONG L P. Analysis of polyphenolic antioxidants in star fruit using liquid chromatography and mass spectrometry[J]. Journal of Chromatography A, 2004, 1022(1): 67-75.
[4] PAIXO N, PERESTRELO R, MARQUES J C, et al. Relationship between antioxidant capacity and total phenolic content of red, rosé and white wines[J]. Food Chemistry, 2007, 105(1): 204-214.
[5] SHEN Yun, JIN Liang, XIAO Peng, et al. Total phenolics, flavonoids, antioxidant capacity in rice grain and their relations to grain color, size and weight[J]. Journal of Cereal Science, 2009, 49(1): 106-111.
[6] 王丽丽, 赵新淮. 紫丁香叶提取物对低密度脂蛋白氧化修饰的抑制作用[J]. 东北农业大学学报, 2007, 38(5): 641-644.
[7] 谷仿丽, 陈乃富, 戴军, 等. 山楂总黄酮和茶多酚联合调节血脂的优化配比研究[J]. 皖西学院学报, 2009, 25(2): 76-78.
[8] 陈晓燕, 邓启盼, 罗非君, 等. EGCG在肿瘤预防及治疗中作用的分子机制[J]. 生命的化学, 2011, 31(2): 207-211.
[9] 彭钰芳, 许传莲. 黄酮类化合物抑制肿瘤细胞增殖活性及构效关系[J]. 浙江理工大学学报, 2011, 28(2): 267-270.
[10] 黄进. 大豆活性成分的结构与功能评价: 肿瘤细胞凋亡、降糖、抗氧化作用[D]. 武汉: 华中农业大学, 2003: 74-93.
[11] 格根塔娜. 蒙药材库页悬钩子木的活性成分研究: 乙酸乙酯层和正丁醇层的活性成分研究[D]. 通辽: 内蒙古民族大学, 2012: 10.
[12] LOZIENE K, VENSKUTONIS P R, SIPAILIENE A, et al. Radical scavenging and antibacterial properties of the extracts from different Thymus pulegioides L. chemotypes[J]. Food Chemistry, 2007, 103(2): 546-559.
[13] 江和源, 柯昌强, 王川丕, 等. 茶籽饼粕中黄酮苷的HPLC分析、制备与MS鉴定[J]. 茶叶科学, 2005, 25(4): 56-61.
[14] 再帕尔·阿不力孜. 天然产物研究方法和技术[M]. 北京: 化学工业出版社, 2010: 5.
[15] 汤坚盛龙生. 液相色谱质谱联用技术在食品和药品分析中的应用[M]. 北京: [出版者不详], 2008: 53.
[16] ASHIHARA H, DENG W W, MULLEN W, et al. Distribution and biosynthesis of flavan-3-ols in Camellia sinensis seedlings and expression of genes encoding biosynthetic enzymes[J]. Phytochemistry, 2010, 71(5/6): 559-566.
[17] 曹永庆, 姚小华, 任华东, 等. 油茶果实矿质元素含量和油脂积累的相关性[J]. 中南林业科技大学学报, 2013, 33(10): 38-41.
[18] 朱勇, 王湘莹, 马锦林, 等. 不同物种油茶籽仁含油率及其茶油的脂肪酸组成[J]. 经济林研究, 2013, 31(2): 134-137.
[19] LEE C, YEN G. Antioxidant Activity and Bioactive Compounds of Tea Seed (Camellia oleifera Abel.) Oil[J]. J. Agric. Food Chem., 2006, 54(3): 779-784.
[20] 王进英, 钟海雁, 朱晓阳, 等. 多齿红山茶籽多酚组分的抗氧活性研究[J]. 食品与机械, 2013, 29(1): 105-107.
[21] LEE C, SHIH P, HSU C, et al. Hepatoprotection of tea seed oil (Camellia oleifera Abel.) against CCl4-induced oxidative damage in rats[J]. Food and Chemical Toxicology, 2007, 45(6): 888-895.
[22] FES X, ESTEVINHO L M, SALINERO C, et al. Triacylglyceride, Antioxidant and Antimicrobial Features of Virgin Camellia oleifera, C. reticulata and C. sasanqua Oils[J]. Molecules, 2013, 18(4): 4 573-4 587.
[23] 李好, 钟海雁, 方学智, 等. 油茶籽成熟过程中抗氧化物质的变化规律[J]. 食品与机械, 2013, 29(5): 6-9.
[24] DU Li-chun, WU Bei-li, CHEN Jian-min. Flavonoid triglycosides from the seeds of Camellia oleifera Abel.[J]. Chinese Chemical Letters, 2008, 19(11): 1 315-1 318.
[25] 刘应珍, 邹天才. 贵州山茶属植物资源及其开发利用探讨[J]. 贵州科学, 2008, 26(2): 75-81.
[26] 田晔林. 湖南省山茶属红山茶组植物分类及园林应用研究[D]. 长沙: 中南林学院, 2001: 33-37.
[27] 廖义秀, 钟海雁. 茶油贮藏的稳定性及高效液相色谱分析[J]. 食品与机械, 2013, 29(1): 173-176.
[28] 漆龙霖, 吕芳德, 李克瑞, 等. 湖南山茶属植物种质资源调查、收集和利用的研究[J]. 武汉植物学研究, 1989, 7(3): 275-284.
[29] 张微微, 于大永, 史丽颖, 等. 南山茶果皮化学成分的研究[J]. 广西植物, 2012, 32(5): 698-700.
[30] 石海峰, 冯宝民, 史丽颖, 等. 西南山茶化学成分的分离与鉴定[J]. 沈阳药科大学学报, 2010, 27(5): 357-360.
[31] 彭晓, 于大永, 冯宝民, 等. 金花茶花化学成分的研究[J]. 广西植物, 2011, 31(4): 550-553.
[32] 佟小静. 油茶根化学成分研究[D]. 苏州: 苏州大学, 2011.
[33] TENG Xi-feng, YANG Jia-yue, YANG Chong-ren, et al. Five New Flavonol Glycosides from the Fresh Flowers of Camellia reticulata[J]. Helvetica Chimica Acta, 2008, 91(7): 1 305-1 312.
[34] 李菁. 茶籽饼抗肿瘤活性成分与茶籽壳抗氧化的初步研究[D]. 武汉: 湖北中医药大学, 2012: 29.
[35] 侯蕾, 于大永, 冯宝民, 等. 西南红山茶花化学成分的分离与鉴定[J]. 时珍国医国药, 2011, 22(7): 1 701-1 703.
[36] SONG Li-xia, WANG Xiang-she, ZHENG Xue-qin, et al. Polyphenolic antioxidant profiles of yellow camellia[J]. Food Chemistry, 2011, 129(2): 351-357.
[37] PARK J S, YEOM M H, PARK W S, et al. Enzymatic hydrolysis of green tea seed extract and its activity on 5alpha-reductase inhibition[J]. Biosci Biotechnol Biochem, 2006, 70(2): 387-394.
[38] LIN L, CHEN P, HARNLY J M. New phenolic components and chromatographic profiles of green and fermented teas[J]. Journal of Agricultural and Food Chemistry, 2008, 56(17): 8 130-8 140.
[39] KIM Y, GOODNER K L, PARK J, et al. Changes in antioxidant phytochemicals and volatile composition of Camellia sinensis by oxidation during tea fermentation[J]. Food Chemistry, 2011, 129(4): 1 331-1 342.
[40] 成桂仁, 金静兰, 文永新. 白水茶中二种新黄酮甙的结构[J]. 药学学报, 1987, 22(3): 203-207.
[41] ZHAO Yang, CHEN Pei, LIN Long-ze, et al. Tentative identification, quantitation, and principal component analysis of green pu-erh, green, and white teas using UPLC/DAD/MS[J]. Food Chemistry, 2011, 126(3): 1 269-1 277.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.