Abstract
The content of polysaccharide was determined by phenol sulfuric acid method. The research investigated FL-3 resin on the adsorption and desorption effect of Gentiana scabra Bunge polysaccharide. FL-3 resin was used to purify the polysaccharide. Using the adsorption rate and desorption amount of polysaccharide as the index, the FL-3 resin purification process of polysaccharide was studied. The optimum conditions for the adsorption and resolution of polysaccharide by FL-3 resin were obtained. The experimental results showed that the polysaccharide can be effectively adsorbed and desorbed by the FL-3 resin. The optimum purification conditions were as follows: the sample concentration was 5 mg/mL, the sample flow rate was 2 BV/h, the sample volume was 4 BV, the elution flow rate was about 2 BV/h, and the concentration of ethanol solution was 30%，the elution volume was 200 mL. After purification, the color of polysaccharide become shallow, and the content of that was increased from 26.42% to 71.29%.The recovery rate of polysaccharide was 49.52%.
Publication Date
5-28-2017
First Page
178
Last Page
181
DOI
10.13652/j.issn.1003-5788.2017.05.036
Recommended Citation
Jiangning, GONG; Yu, RAO; Yiju, YANG; and Fei, CHEN
(2017)
"Optimization on adsorption and purification process for gentian polysaccharide in Guizhou by macroporous resin,"
Food and Machinery: Vol. 33:
Iss.
5, Article 36.
DOI: 10.13652/j.issn.1003-5788.2017.05.036
Available at:
https://www.ifoodmm.cn/journal/vol33/iss5/36
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