Structure determination on a new acetyl flavonoid glycoside in seeds of Camellia semiserrata Chi. by NMR spectroscopy

Authors

CAO Qingming, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees 〔Central South University of Forestry and Technology〕, Ministry of Education, Changsha, Hunan 410004, China
LAN Fang, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
WANG Weijie, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
BAO Liyuan, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
ZHONG Haiyan, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees 〔Central South University of Forestry and Technology〕, Ministry of Education, Changsha, Hunan 410004, China

Abstract

A high-purity compound was extracted by solvent extraction, liquid-liquid separation, silica gel chromatography and high-performance liquid semi-preparative chromatography by using seeds of Camellia semiserrata Chi. as raw materials. The compound was identified as kaempferol-3-O-\[α-L-rhamnopyranosyl-(1-3)- 2,4-di-O-acetyl-α-L-rhamnopyranosyl-(1-6)\]-β-D-glucopyranoside, an acetyl flavonoid glycoside, by physical and chemical properties, mass spectrometry and nuclear magnetic resonance. Two-dimensional nuclear magnetic resonance (2D-NMR) techniques including H-H COSY, HSQC, HMBC and TOCSY were used to affiliate its ¹H and ¹³C NMR chemical shift and explain the structure.

Publication Date

5-28-2017

First Page

21

Last Page

24,58

DOI

10.13652/j.issn.1003-5788.2017.05.004

Recommended Citation

Qingming, CAO; Fang, LAN; Weijie, WANG; Liyuan, BAO; and Haiyan, ZHONG (2017) "Structure determination on a new acetyl flavonoid glycoside in seeds of Camellia semiserrata Chi. by NMR spectroscopy," Food and Machinery: Vol. 33: Iss. 5, Article 4.
DOI: 10.13652/j.issn.1003-5788.2017.05.004
Available at: https://www.ifoodmm.cn/journal/vol33/iss5/4

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