Morphological and molecular identification of four groups desert green algae

Authors

QELBINUR Eyit, School of Life Science, Xinjiang Normal University, Urumqi, Xinjiang 830054, China; Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, Urumqi, Xinjiang 830054, China
AISHANJIAN G, School of Life Science, Xinjiang Normal University, Urumqi, Xinjiang 830054, China; Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, Urumqi, Xinjiang 830054, China
NURGUL Rahman, School of Life Science, Xinjiang Normal University, Urumqi, Xinjiang 830054, China; Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, Urumqi, Xinjiang 830054, China

Abstract

Four groups of algas, isolated from the Taklimakan desert, were identified by morphological characters in this study. The pH effect of the growth of algae is analyzed according to the growth curve, and the phylogenetic tree was pictured. It shows that the group a belongs to the oval algae, and group b, c and d belong to the spherical algae. The optimum suitable pH values for the group a desert algae was 6.0, 8.0 for the group b and d; the same value of original BG-11 for the group c. Four representative strains of desert algae gene sequences were identified by using the 18S rDNA sequence analysis method. It shows that the strain of 3YS16① (group a) and the strain of 3YS21-1 (group c) have close genetic relationship with the Chlamydomonas incerta; the strain of KXII(2) (group b) has close genetic relationship with the Chlorosarcinopsis bastropiensi; the Strain of YS2-3 (group d) has close genetic relationship with Chlorosarcinopsis communis. This study determined the classification status of four groups of algae and the optimal pH growth conditions, and provided theoretical basis to the molecular biology studies of desert algae and applications in food industry.

Publication Date

5-28-2017

First Page

25

Last Page

29

DOI

10.13652/j.issn.1003-5788.2017.05.005

Recommended Citation

Eyit, QELBINUR; G, AISHANJIAN; and Rahman, NURGUL (2017) "Morphological and molecular identification of four groups desert green algae," Food and Machinery: Vol. 33: Iss. 5, Article 5.
DOI: 10.13652/j.issn.1003-5788.2017.05.005
Available at: https://www.ifoodmm.cn/journal/vol33/iss5/5

References

[1] SMITH S M, ABED R M M, GARCIA PICHEL F, et al. Biological soil crusts of sand dunes In cape cod national seashore Massachusetts, USA[J]. Microbial Ecology, 2004, 48: 200-208.
[2] HAWKES C V, FLECHTNER V R. Biological soil crosts In a Xeric Florlda Shrubland: composition, abundance, and spatial heterogeneity of crusts with different disturbance histories[J]. Microbial Ecology, 2002, 43(1): 1-12.
[3] 王静, 林向阳, 李麒龙, 等. 环保型微藻絮凝剂的制备及应用[J]. 食品与机械, 2014, 30(2): 206-210.
[4] 唐翱. SY绿藻产品推广项目营销策略研究[D]. 成都: 西南交通大学, 2006: 14-15.
[5] 依里夏提·地里夏提, 艾山江, 吾甫尔·米吉提. 3株塔克拉玛干沙漠藻的分子鉴定与分析[J]. 安徽农业科学, 2015, 43(18): 41-44, 48.
[6] 李芳芳, 隋正红, 龚春霞. 5种沙漠微藻的分离鉴定及其18S rDNA保守区片段差异分析[J]. 石河子大学学报: 自然科学版, 2012, 30(3): 265-270.
[7] 赵建成, 张丙昌, 张元明. 新疆古尔班通古特沙漠生物结皮绿藻研究[J]. 干旱区研究, 2006, 23(2): 189-194.
[8] 李芳芳, 隋正红, 龚春霞, 等. 5种沙漠微藻的分离鉴定及其18S rDNA保守区片段差异分析[J]. 石河子大学学报: 自科版, 2012, 30(3): 265-270.
[9] SHIELDS L M, DURREL LW. Algae in relation to soil fertility[J]. The Botanica Review, 1964, 30(1): 92-128.
[10] EVANS C T, RATLEDGE C. Influence of nitrogen metabolism of lipid accumulation by rhodosporidium tor loides CBS14[J]. Journal of General Microbiology, 1984, 130(7): 1 704-1 705.
[11] 林玉艳. 一株海洋微藻的分离鉴定及环境因素对其生长的影响[D]. 青岛: 中国海洋大学, 2015.
[12] 刘欣, 姚卫蓉. 藻类食品卫生指标解析[J]. 食品科技, 2013(12): 299-303.
[13] 吉宏武, 赵素芬. 南海3种可食绿藻化学成分及其营养评价[J]. 广东海洋大学学报, 2005, 25(3): 19-23.
[14] HOHN S, HALLMANN A.There is more than one way to turn aspherical cellular monolayer inside out: Type B embryo inversion in Voluox globator[J]. Biomedcentral, 2011, 29(9): 89.
[15] 周楠. 水体中微囊藻毒素的分析提纯及去除实验研究[D]. 哈尔滨: 哈尔滨工业大学, 2009: 31.
[16] OLMOS J, PANIAGUA J, CONTRERAS R. Molecular identication of Dunaliella sp. utilizing the 18S rDNA gene[J]. Letters in Applied Microbiology, 2000, 30: 80-84.
[17] 卢梦瑶, 刘书亮, 胡凯弟, 等. 酵素中一株高产酸醋酸菌的鉴定及其产酸特性[J]. 食品与机械, 2016, 32(12): 64-69, 114.
[18] KUMAR S, NEI M, DUDLEY J, et al. MEGA: A biologistcentric software for evolutionary analysis of DNA and protein sequences[J]. Briefings in Bioinformatics, 2008, 9(4): 299-306.
[19] 李芳芳, 隋正红, 周伟. 两种沙漠微藻的形态学和分子生物学鉴定[J]. 干旱区资源与环境, 2013(3): 167-172.
[20] 龚春霞, 王丹, 苟亚峰. 4株沙漠土壤衣藻的分离培养及形态和分子鉴定[J]. 石河子大学学报: 自然科学版, 2013, 31(3): 294-300.
[21] 徐娜, 逄少军. 三种海洋浮游微藻的分子鉴定及培养条件研究[J]. 海洋科学, 2012, 36(10): 13-25.
[22] 尹乐斌, 张臣飞, 孙菁, 等. 一株产细菌素乳酸菌的分离、鉴定及生物学特性研究[J]. 食品与机械, 2016, 32(3): 12-15, 64.
[23] 王雪青, 赵培, 胡萍, 等. 金藻3011的培养条件优化及扩大培养研究[J]. 食品科学, 2006(12): 253-259.
[24] 李艳红. 环境因子对铜绿微囊藻生长和光合作用的影响[D]. 南昌: 南昌大学, 2010: 40.
[25] 袁丽娜, 宋炜, 肖琳. 多环境因素全面正交作用对铜绿微囊藻生长的效应研究[J]. 南京大学学报: 自然科学版, 2008(4): 408-414.
[26] 赵娜, 冯鸣凤, 朱琳. 不同pH值条件下Cr⁶⁺对小球藻和斜生栅藻的毒性效应[J]. 东南大学学报: 医学版, 2010(4): 382-386.
[27] 欧阳峥嵘, 温小斌, 耿亚红. 光照强度、温度、pH、盐度对小球藻(Chlorella)光合作用的影响[J]. 武汉植物学研究, 2010(1): 49-55.
[28] 侯建军, 赖红艳, 黄邦钦. 几种典型藻华生物的分子分类学分析[J]. 水生生物学报, 2008, 32(5): 711-719.
[29] 林子杰. 三种绿藻的ITS和18S rDNA序列及系统发育分析[D]. 苏州: 苏州大学, 2011: 23-31.
[30] 胡乐琴, 唐晨, 汪卿. 前沟藻18S rDNA序列克隆和分子鉴定分析[J]. 生物技术通报, 2011(12): 92-95.
[31] 庄丽, 陈月琴, 李钦亮. 赤潮叉角藻18S rDNA和ITS区序列测定与分析[J]. 海洋与湖沼, 2001, 32(2): 148-154.
[32] 王丹, 龚春霞, 苟亚峰. 塔克拉玛干沙漠生物结皮中几种藻类的系统发育分析[J]. 草业学报, 2014(3): 97-103.
[33] LEWIS L A, LEWIS P O. Unearthing the molecular phylodiversity of desert soil green algae (Chlorophyta)[J]. Systems Biology, 2005, 54(6): 936-947.