Abstract
By applying surface-enhanced Raman spectroscopy (SERS), a rapid detection of Kitasamycin (KIT) residues in duck meat was proposed. On the bases of analyzing UV-visible absorption spectrum of enhanced substrate and SERS of KIT in duck meat, the SERS detection conditions of KIT in duck meat, the addition amount of duck meat extract containing KIT and adsorption time, were optimized by single factor method, and the standard curves for the detection of KIT aqueous solution and KIT residues in duck meat were build respectively. The experiment results showed that the standard curve equation and the coefficient of determination were y=0.046 2x+0.215 3 and 0.927 9 respectively for the detection of KIT in aqueous solution with the average recovery rate of 99%~123%. The standard curve equation and the coefficient of determination were y=0.011 9x+0.940 9 and 0.923 2 respectively for the detection of KIT residues in duck meat with the average recovery rate of 104%~108%.The experiment results showed that it was feasible to detect the KIT residues in duck meat by using SERS technology.
Publication Date
6-28-2017
First Page
60
Last Page
63
DOI
10.13652/j.issn.1003-5788.2017.06.012
Recommended Citation
Jinhui, ZHAO; Qian, HONG; Haichao, YUAN; and Muhua, LIU
(2017)
"Detection of kitasamycin residues in duck meat using surface-enhanced Raman spectroscopy,"
Food and Machinery: Vol. 33:
Iss.
6, Article 12.
DOI: 10.13652/j.issn.1003-5788.2017.06.012
Available at:
https://www.ifoodmm.cn/journal/vol33/iss6/12
References
[1] 张楠. 替米考星在肉鸭体内生物利用度及组织分布研究[D]. 武汉: 华中农业大学, 2008.
[2] 邬立刚. 不同剂型吉他霉素对肉仔鸡生产性能的影响[J]. 饲料与畜牧, 2012, 21(9): 31-33.
[3] 刘晔. 动物性食品中大环内醋类抗生素残留的HPLC分析[D]. 无锡: 江南大学, 2007: 13-22.
[4] 黄晓蓉, 郑晶, 吴谦, 等. 食品中多种抗生素残留的微生物筛检方法研究[J]. 食品科学, 2007, 28(7): 418-421.
[5] TAKASUKI K, USHIZAWA I, SHOJI T. Gas chromatogra-phy-mass spectrometric determination of macrolide in beef and pork using single ion monitoring[J]. Chromatogr, 1987, 391: 207-217.
[6] HORIE M, HARUMI T, KAZUO T. Determination of macrolide antibiotics in meat and fish by liquid chromatography-electrospray mass spectrometry[J]. Acta Analytica Chimica Acta, 2003, 492(1/2): 187-197.
[7] TAN Hong-liang, CHEN Yang. Silver nanoparticle enhanced fluorescence of enropium(Ⅲ) for detection of tetracycline in milk[J]. Sensors and Actuators B: Chemical, 2012, 173: 262-267.
[8] LI Chun-ying, HUANG Yi-qun, PEI Lu, et al. Analyses of Trace Crystal Violet and Leucocrystal Violet with Gold Nanospheres and Commercial Gold Nanosubstrates for Surface-Enhanced Raman Spectroscopy[J]. Food Anal. Methods, 2014, 7(10): 2 107-2 112.
[9] ZHANG Yuan-yuan, YU Wan-song, PEI Lu, et al. Rapid analysis of malachite green and leucomalachite green in fish muscles with surface-enhanced resonance Raman scattering[J]. Food Chemistry, 2015, 169: 80-84.
[10] ZHAI Fu-li, HUANG Yi-qun, LI Chun-ying, et al. Rapid Determination of Ractopamine in Swine Urine Using Surface-Enhanced Raman Spectroscopy[J]. Journal of Agricultural and Food Chemistry, 2011, 59(18): 10 023-10 027.
[11] 朱自莹, 顾仁敖, 陆天虹. 拉曼光谱在化学中的应用[M]. 沈阳: 东北大学出版社, 1998: 295-307.
[12] 许以明. 拉曼光谱及其在结构生物学中的应用[M]. 北京: 化学工业出版社, 2005: 90-124.