Abstract
The effects of pulsed electric field on chelating of ovalbumin and metal ions (Cu2+, Ba2+, Mn2+ or Ca2+) and the effect of pulsed electric field on the chelating effect of ovalbumin were studied by Raman spectroscopy. Results: ① PEF treatment time and metal ion species could affect the chelation of metal ions and protein, and the chelation of metal ions with ovalbumin was enhanced with the prolongation of pulse treatment time, when PEF treatment time was 1 695 μs, the chelation of Mn2+ and Cu2+ with protein began to weaken; ② The protein and metal ions chelated to different degrees at 1 200～1 700 cm-1, the strongest was Mn2+ , followed by Cu2+ and Ca2+, and the weakest was Ba2+.
Publication Date
6-28-2017
First Page
13
Last Page
16
DOI
10.13652/j.issn.1003-5788.2017.06.003
Recommended Citation
Ting, TANG; Yanyan, LIU; Aimin, JIANG; and Zhuo, JIANG
(2017)
"Study on chelating of metal ion and protein in pulsed electric field,"
Food and Machinery: Vol. 33:
Iss.
6, Article 3.
DOI: 10.13652/j.issn.1003-5788.2017.06.003
Available at:
https://www.ifoodmm.cn/journal/vol33/iss6/3
References
[1] LIU Yan-yan, ZHANG Ying, ZENG Xin-an, et al. Effect of Pulsed Electric Field on Microstructure of Some Amino Acid Group of Soy Protein Isolates[J]. International Journal of Food Engineering, 2014, 10(1): 113-120.
[2] 曾新安, 资智洪, 杨连生. 高强脉冲电场对食品成分的影响[J]. 食品工业科技, 2008, 29(12): 256-259.
[3] ZHAO Wei, TANG Ya-li, LU Li-xin, et al. Review: Pulsed Electric Fields Processing of Protein-Based Foods[J]. Food and Bioprocess Technology, 2014, 7(1): 114-125.
[4] 颜文旭, 张姗姗, 公群. 高压脉冲电场杀菌多物理场特性研究[J]. 食品与机械, 2016, 32(2): 1-6.
[5] ZHANG Qing-hua, BARBOSACANOVAS G V, SWANSON B G. Engineering aspects of pulsed electric-field pasteurization[J]. Journal of Food Engineering, 1995, 25: 261-281.
[6] 夏涛, 吴云峰, 王胜利, 等. 高压脉冲电场杀菌系统中高压脉冲发生器研究进展[J]. 食品与机械, 2016, 32(5): 229-231.
[7] 曾新安, 刘燕燕, 李云, 等. 高强脉冲电场和热处理对橙汁维生素C影响比较[J]. 食品工业科技, 2009, 30(6): 123-124.
[8] 赵伟, 杨瑞金, 张文斌, 等. 高压脉冲电场对食品中微生物、酶及组分影响的研究进展[J]. 食品与机械，2010, 26(3): 153-157.
[9] 卢沿钢, 董全. 高压脉冲电场提取食品中天然产物的研究进展[J]. 食品与机械, 2012, 28(1): 243-246.
[10] 李乐军. 荧光光谱与激光拉曼光谱研究脉冲电场对蛋白质构象的影响[D]. 上海: 华东师范大学, 2005: 1-3.
[11] 贾晓, 曾新安. 拉曼光谱研究脉冲电场作用下磷脂脂质体构象的变化[J]. 食品工业科技, 2012, 33(5): 138-140.
[12] 张芳, 林东海. 用核磁共振方法研究金属离子与蛋白质的相互作用[J]. 波谱学杂志, 2009, 26(1): 136-149.
[13] 刘西海. 金属离子对蛋清蛋白质结构的影响研究[J]. 中国家禽, 2012, 34(1): 27-31.
[14] 赵南明. 生物物理学[M]. 北京: 高等教育出版社, 2000: 215-216.
[15] 王中江, 江连洲. 大豆分离蛋白在不同pH下的拉曼光谱分析[J]. 食品工业科技, 2012, 33(11): 65-70.
[16] 孙娟, 张晖, 王立, 等. 基于拉曼光谱的大米快速分类判别方法[J]. 食品与机械, 2016, 32(1): 41-45.
[17] 袁丽, 纪秀, 石彤, 等. 拉曼光谱法分析凡纳滨对虾冻藏过程蛋白质与水分结构变化[J]. 食品科学, 2016, 37(18): 202-207.
[18] HERRERO A M, CARMONA P, GARCIA M L, et al. Ultrastructural changes and structure and mobility of myowater in frozen-stored hake (Merluccius merluccius L.) muscle: relationship with functionality and texture[J]. Journal of Agricultural and Food Chemistry, 2005, 53(7): 2 558-2 566.
[19] 李乐军, 陈树德, 乔登江. 脉冲电场与牛血清白蛋白相互作用的同步荧光光谱和拉曼光谱比较研究[J]. 光谱学与光谱分析, 2006(1): 81-86.
[20] 黄群, 金永国, 马美湖, 等. 超高压处理对S-卵白蛋白构象与功能特性的影响[J]. 农业机械学报, 2013, 44(3): 161-166.
[21] CRAIG W S，GABER B P. Laser Raman scattering from an enzyme of well-documented structure，human carbonic anhydrase B[J]. J. Am. Chem. Soc., 1977, 99(12): 4 130-4 134.