Purification of tea saponin with macroporous resin and properties of the product

Authors

GU Jiao, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;State Key Laboratory of Food Science &Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
YANG Ruijin, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;State Key Laboratory of Food Science &Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
XIE Bin, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;State Key Laboratory of Food Science &Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
ZHANG Wenbin, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;State Key Laboratory of Food Science &Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
ZHAO Wei, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;State Key Laboratory of Food Science &Technology, Jiangnan University, Wuxi, Jiangsu 214122, China
HUA Xiao, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;State Key Laboratory of Food Science &Technology, Jiangnan University, Wuxi, Jiangsu 214122, China

Abstract

Purification of tea saponin with macroporous resin and the changes of properties of the product were investigated. With comparisons of eight kinds of macroporous resin with different polarity to the crude tea saponin through static adsorption and dynamic adsorption, XR910X resin was selected to refine the tea saponin. Moreover, it was found that the tea saponin adsorption by XR910X would be matched by Freundlich Model. The optimal purification conditions were as following: the loading sample was crude tea saponin solution with pH 6.0, and the loading volume 1 BV, loading velocity 1.71 BV/h. Then 0.05 g/100 mL NaOH solution was used to remove impurity with 1.71 BV/h velocity and 2 BV volume. At last, 90% ethanol of 2 BV volume was used as eluent to desorb the tea saponin with the ethanol flow rate 1.71 BV/h. Under that condition, the recovery rate of tea saponin was 70.34% and the purity was 94.26%. In addition, the properties of tea saponin after purification in face activity and antioxidant capacity as well as antibacterial activities were much higher than those before purification.

Publication Date

6-28-2017

First Page

153

Last Page

158,200

DOI

10.13652/j.issn.1003-5788.2017.06.031

Recommended Citation

Jiao, GU; Ruijin, YANG; Bin, XIE; Wenbin, ZHANG; Wei, ZHAO; and Xiao, HUA (2017) "Purification of tea saponin with macroporous resin and properties of the product," Food and Machinery: Vol. 33: Iss. 6, Article 31.
DOI: 10.13652/j.issn.1003-5788.2017.06.031
Available at: https://www.ifoodmm.cn/journal/vol33/iss6/31

References

[1] 中国产业调研网. 2015-2020年中国油茶行业现状调研分析与发展趋势预测报告[R/OL]. 2017版. [2017-06-11]. http://www.cir.cn/R_ShiPinYinLiao/85/YouChaHangYeXianZhuang YanJ-iu. html.
[2] 王曙跃. 一种性能优良的天然表面活性剂—茶皂素[J]. 日用化学工业, 1987(2): 32-34.
[3] XIA Chun-hua, ZHU Quan-fen, TIAN Jie-hua et al. Surface Activity of Tea Saponin and the Related Functional Properties[J]. Journal of Tea Science, 1990, 10(1): 1-10.
[4] CHEN Jiao-jiao, ZHANG Shao-shan, YANG Xiao-ping. Control of brown rot on nectarines by tea polyphenol combined with tea saponin[J]. Crop Protection, 2013(45): 29-35.
[5] ZHANG Xin-fu, YANG Shao-lan, HAN Ying-ying, et al. Qualitative and quantitative analysis of triterpene saponins from tea seed popace (Camellia oleifera abel) and their activities against bacteria and fungi[J]. Molecules, 2014, 19(6): 7 568-7 580.
[6] SUR P, CHAUDHURI T, VEDASIROMONI J R, et al. Antiinflammatory and antioxidant property of saponins of tea [Camellia sinensis (L) O. Kuntze] root extract[J]. Phytotherapy Research, 2001, 15(2): 174-176.
[7] CHATTOPADHYAY P, BESRA S E, GOMES A, et al. Anti-inflammatory activity of tea (Camellia sinensis) root extract[J]. Life sciences, 2004, 74(15): 1 839-1 849.
[8] MATSUI Y, KOBAYASHI K, MASUDA H, et al. Quantita-tive analysis of saponins in a tea-leaf extract and their antihypercholesterolemic activity[J]. Bioscience, Biotechnology, and Biochemistry, 2009, 73(7): 1 513-1 519.
[9] GHOSH P, BESRA S E, TRIPATHI G, et al. Cytotoxic and apoptogenic effect of tea (Camellia sinensis var. assamica) root extract (TRE) and two of its steroidal saponins TS1 and TS2 on human leukemic cell lines K562 and U937 and on cells of CML and ALL patients[J]. Leukemia Research, 2006, 30(4): 459-468.
[10] 徐先祥, 夏伦祝, 高家荣. 大孔吸附树脂在皂苷分离纯化中的应用[J]. 中国中医药信息杂志, 2003, 10(1): 79-80.
[11] 李强. 乙醇辅助水剂法提取油茶籽油[D]. 无锡: 江南大学, 2012: 8-30.
[12] 游瑞云, 黄雅卿, 郑珊瑜, 等. 大孔树脂纯化茶皂素的工艺研究[J]. 应用化工, 2016(1): 64-66, 70.
[13] 刘红梅, 周新跃, 周建平, 等. 油茶皂素定量分析的研究[J]. 生命科学仪器, 2008, 6(10): 13-16.
[14] 王延芳. 油茶皂素及其水解产物的分离及降血脂抗氧化活性研究[D]. 广州: 华南理工大学, 2012: 57-58.
[15] 赵丽. 油茶枯中抑菌物质的提取及其抑菌活性研究[D]. 广州: 仲恺农业工程学院, 2015: 25-27.
[16] 苏鹤, 杨瑞金, 赵伟, 等. 紫薯花色苷的超滤和树脂联用纯化工艺[J]. 食品工业科技, 2016(10): 268-272.
[17] 孙国金, 叶春林, 余勤. D101型大孔树脂纯化茶皂素的研究[J]. 食品科技, 2011, 36(3): 206-208.
[18] 孟维, 李湘洲, 吴志平, 等. AB-8型大孔吸附树脂分离纯化茶皂素工艺[J]. 食品科技, 2013(9): 213-217.
[19] 娄嵩, 刘永峰, 白清清, 等. 大孔吸附树脂的吸附机理[J]. 化学进展, 2012(8): 1 427-1 436.
[20] 欧阳玉祝, 张辞海, 魏燕. 大孔树脂对倍花单宁酸的吸附特性及其动力学模型[J]. 食品科学, 2013(11): 122-125.
[21] 孙君辉, 孟秀芬. 大孔树脂的应用及其提取纯化中药有效成分的影响因素[J]. 山东商业职业技术学院学报, 2008(6): 94-96.
[22] 米聪. 紫薯中花色苷的提取纯化及稳定性研究[D]. 厦门: 集美大学, 2014: 36-37.
[23] 彭青, 李晓刚, 刘亚明. 大孔吸附树脂研究进展[J]. 实用中医药杂志, 2013(5): 409-412.
[24] 程文娟, 谢海荣, 秦永, 等. 膜分离与大孔树脂联用技术纯化茶皂素[J]. 食品与机械, 2015, 31(4): 172-177.
[25] 王兰. 茶籽粕中茶皂素的提取及性质表征[D]. 西安: 陕西科技大学, 2012: 3-5.