Abstract
In the present study, six total flavonoids extracts were prepared from Honey suckle, Walnut leaves, Withania Somnifera, Rhodiola rosea, Pomegranate bark, and Polygonum multiflorum. The total flavonoids content of six plant extracts were measured by aluminium nitrate-sodium nitrite colorimetry. The antioxidant activities of six extracts were compared by the detection methods of DPPH, ABTS and Fenton, and then the correlation of total flavonoids content was analyzed by Pearson. The results showed that the DPPH scavenging order was Honey suckle＞Walnut leaves＞W. Somnifera＞R. rosea＞P. multiflorum＞Pomegranate bark. The hydroxyl radical scavenging order was Honey suckle＞Walnut leaves＞W. Somnifera＞P. multiflorum＞Pomegranate bark＞Rhodiola rosea. The results showed that the ABTS+ scavenging order was Honey suckle＞Walnut leaves＞R. rosea＞W. Somnifera＞P. multiflorum＞Pomegranate bark. The free radical scavenging ability and flavonoids content of six kinds of extracts showed quantitative relationship. The correlation that the total flavonoid content and DPPH, ABTS+ was significantly positive, while with hydroxyl radical was not the same. The correlation coefficients above were 0.819, 0.848 and 0.791, respectively.
Publication Date
6-28-2017
First Page
159
Last Page
163
DOI
10.13652/j.issn.1003-5788.2017.06.032
Recommended Citation
Jingjing, FU; Haifang, XIAO; and Yuanda, SONG
(2017)
"Acomparative study of contents of total flavonoids and their antioxidant activities in six plants,"
Food and Machinery: Vol. 33:
Iss.
6, Article 32.
DOI: 10.13652/j.issn.1003-5788.2017.06.032
Available at:
https://www.ifoodmm.cn/journal/vol33/iss6/32
References
[1] GOMBERG M. An instance of trivalent carbon: triphenylmethyl[J]. Journal of the American Chemical Society, 1900, 22(11): 757-771.
[2] SALLA S, SUNKARA R, OGUTU S, et al. Antioxidant activi-ty of papaya seed extracts against H2O2, induced oxidative stress in HepG2 cells[J]. LWT-Food Science and Technology, 2016, 66(1): 293-297.
[3] PHANIENDRA A, JESTADI D B, PERIYASAMY L. Free radicals: properties, sources, targets, and their implication in various diseases[J]. Indian Journal of Clinical Biochemistry, 2015, 30(1): 11-26.
[4] MARNETT L J. Oxyradicals and DNA damage[J]. Carcinogenesis, 2000, 21(3): 361-370.
[5] STADTMAN E R, LEVINE R L. Protein oxidation[J]. Annals of the New York Academy of Sciences, 2000, 899(1): 191-208.
[6] SEPPO Yl-herttuala. Oxidized LDL and Atherogenesis[J]. Annals of the New York Academy of Sciences, 2006, 874(1): 134-137.
[7] HAKKIM F L, SHANKAR C G, GIRIJA S. Chemical composition and antioxidant property of holy basil (Ocimum sanctum L.) leaves, stems, and inflorescence and their in vitro callus cultures[J]. Journal of Agricultural & Food Chemistry, 2007, 55(22): 9 109-9 117.
[8] 李爱军, 欧仕益, 罗泽榕, 等. 一种天然抗氧化剂复合物抗氧化作用的研究[J]. 食品与机械, 2001, 17(4): 28-30.
[9] POLITEO O, JUKIC M, MILOS M. Chemical composition and antioxidant capacity of free volatile aglycones from basil (Ocimum basilicum L.) compared with its essential oil[J]. Food Chemistry, 2007, 101(1): 379-385.
[10] YANG C, GUNDALA SR, MUKKAVILLI R, et al. Synergisticinteractions among flavonoids and acetogenins in Graviola(Annona muricata) leaves confer protection against prostatecancer[J]. Carcinogenesis, 2015, 36(6): 656-665.
[11] BRICKMAN A M, KHAN U A, PROVENZANO F A, et al. Enhancingdentate gyrus function with dietary flavanols improvescognition in older adults[J]. Nat Neurosci, 2014, 17(12): 1 798-1 803.
[12] GONZALEZ R, BALLESTER I, LOPEZ-POSADAS R, et al. Effects of flavonoids and other polyphenols on inflammation[J]. Critical Reviews in Food Science and Nutrition, 2011, 51(4): 331-62.
[13] PASTORELLO EA, CONTI A, PRACETTONI V, et al. Identification of actinidin as the major allergen of kiwifruit[J]. J Allergy Clin Immu, 1998, 101(4): 531-537.
[14] MOAWAD A, HETTA M, ZJAWIONY J K, et al. Phytochemical investigation of Cycas circinalis and Cycas revoluta leaflets: moderately active antibacterial biflavonoids.[J]. Planta Medica, 2010, 76(8): 796.
[15] DURY L, NASR R, LORENDEAU D, et al. Flavonoid dimers are highly potent killers of multidrug resistant cancer cells overexpressing MRP1[J]. Biochemical Pharmacology, 2017, 124： 10-18.
[16] RICE-EVANS C A, MILLER N J, PAGANGA G. Structure-antioxidant activity relationships of flavonoids and phenolic acids[J]. Free Radic. Biol. Med., 1996, 20(1): 933-956.
[17] 熊皓平, 杨伟丽, 张友胜, 等. 天然植物抗氧化剂的研究进展[J]. 天然产物研究与开发, 2001, 13(5): 75-79.
[18] 王春霞, 蒲彪, 蒋燕, 等. 藤椒冷榨油饼粕中黄酮类物质的提取及体外抗氧化活性研究[J]. 食品与机械, 2017, 33(1): 137-142.
[19] 张赟彬, 李彩侠, 吴亚卿. 黄酮类化合物的研究进展[J]. 食品与机械, 2005, 21(5): 70-73.
[20] 刘昌平. 金银花黄酮的抗氧化活性分析[J]. 安徽农业科学, 2009, 37(20): 9 483-9 484.
[21] 陈红红, 李助乐, 章健, 等. 山核桃叶总黄酮抗衰老作用的研究[J]. 天然产物研究与开发, 2008, 1(5): 892-895.
[22] 龚晓武, 李炳奇, 刘丹丹, 等. 红景天黄酮提取及其抗氧化活性研究[J]. 西北林学院学报, 2011, 26(3): 136-138.
[23] 王涛, 郝云辉, 马晶军. 微波辅助提取何首乌中的总黄酮及其抗氧化性研究[J]. 河北医药, 2014, 36(23): 3 654-3 656.
[24] 刘梦星, 刘祺凤, 田璐阳, 等. 石榴皮中总黄酮超声辅助提取及抗氧化性分析[J]. 湖北农业科学, 2014, 53(4): 894-896.
[25] 朱海升, 刘鄂湖, 鞠娟, 等. 抗老年性痴呆的天然药物研究进展[J]. 中国药房, 2007, 18(3): 223-225.
[26] 郭亚健, 范莉. 关于NaNO2-Al(NO3)3-NaOH比色法测定总黄酮方法的探讨[J]. 药物分析杂志, 2002, 1(2): 97-99.
[27] 洪艳平, 陈木森, 上官新晨, 等. 枸骨叶总黄酮超声辅助提取与测定[J]. 食品与机械, 2009, 25(1): 72-75.
[28] 施英, 徐玉娟, 陈卫东, 等. 桔核中柠檬苦素类物质最佳提取条件的探讨及清除DPPH活性的研究[J]. 食品与机械, 2006, 22(6): 74-76.
[29] 马博, 张婷婷, 黎远成, 等. 麻疯树籽壳总黄酮的提取及其羟基自由基清除作用[J]. 食品与机械, 2014, 30(5): 196-199.
[30] 欧阳凯, 何先元, 陈飞, 等. 四齿四棱草总皂苷提取工艺优化及抗氧化性研究[J]. 食品与机械, 2016, 32(7): 141-145.
[31] 唐福才, 关天旺, 姚敦琛, 等. 微波提取龙眼核中多酚及其抗氧化活性的研究[J]. 广东化工, 2015, 42(5): 176-177.
[32] 贾之慎, 邬建敏, 唐孟成. 比色法测定Fenton反应产生的羟自由基[J]. 生物化学与生物物理进展, 1996, 23(2): 184-186.
[33] 郭小补, 廖森泰, 刘吉平, 等. 不同桑品种的桑叶总黄酮含量与体外抗氧化活性的相关性[J]. 蚕业科学, 2008, 34(3): 381-386.