Abstract
In order to study the anti-microbial effect of the six kinds of tea under the same processing raw materials, the spring tea fresh leaves of C.sinensis cv.Bixiangzao were processed into fixation of fresh leaves, green, yellow, dark, white, oolong, and black tea. The anti-microbial activity against Staphylococcus aureus (S. aureus), Escherichia coli (B. subtilis), Escherichia coli(E. coli), Aspergillus niger,(A. niger), Saccharomyces cerevisiae (S. cerevisiae) of 7 samples were determinded by the Oxford cup method. The Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) of 7 samples were determined by the method of two times dilution using the microplate of the 96 hole. Experimental results showed that 7 samples had inhibition effect of bacteria and had no obvious effect of A. niger and S. cerevisiae. The antibacterial circle size of 7 samples of S. aureus, B. subtilis, and E. coli showed that the trend of antimicrobial activity was: fixation of fresh leaves>green>yellow>dark>white>oolong>black tea. The MIC showed that the trend of antimicrobial concentration was: black tea>oolong, white tea>dark, yellow, green tea, fixation of leaves. The MBC showed that the trend of antimicrobial concentration was: black tea>oolong, white, dark, yellow tea>green tea, fixation of leaves. The antimicrobial activity of green tea and black tea was significantly different from other teas (P<0.05).
Publication Date
7-28-2017
First Page
47
Last Page
50,76
DOI
10.13652/j.issn.1003-5788.2017.07.010
Recommended Citation
Yingqi, PENG; Jun, LONG; Ling, LIN; Zhihua, GONG; Dongyin, YUAN; Yang, ZHOU; and Wenjun, XIAO
(2017)
"Comparison on anti-microbial activities of six kinds of teas processed by the same raw materials,"
Food and Machinery: Vol. 33:
Iss.
7, Article 10.
DOI: 10.13652/j.issn.1003-5788.2017.07.010
Available at:
https://www.ifoodmm.cn/journal/vol33/iss7/10
References
[1] 宋丽, 丁以寿. 陈椽茶叶分类理论[J]. 茶业通报, 2009(3): 143-144.
[2] 申雯, 黄建安, 李勤, 等. 茶叶主要活性成分的保健功能与作用机制研究进展[J]. 茶叶通讯, 2016(1): 8-13, 65.
[3] 任蕾, 杨圣辉, 果梅英. 六种茶叶对口腔常见致病菌的抑菌作用[J]. 北京口腔医学, 2003(2): 91-93,117.
[4] 傅冬和, 余智勇, 黄建安, 等. 不同年份茯砖茶水提取物的抑菌效果抑菌[J]. 中国茶叶, 2011(1): 10-12.
[5] 薛晨. 原料级别和贮藏时间对普洱茶品质及其生物活性影响的研究[D]. 合肥: 安徽农业大学, 2013: 37-39.
[6] 王静, 戚向阳, 朱学良, 等. 表没食子儿茶素没食子酸酯及其不同氧化级分的抑菌活性研究[J]. 天然产物研究与开发, 2008(1): 91-94, 62.
[7] 张华艳, 戚丽, 张正竹. 咖啡碱对茶树主要叶部病原真菌的抑制作用[J]. 南京农业大学学报, 2010(2): 63-67.
[8] 金恩惠, 吴媛媛, 屠幼英. 茶黄素抑菌作用的研究[J]. 中国食品学报, 2011(6): 108-112.
[9] 方世辉, 张秀云, 夏涛, 等. 茶树品种、加工工艺、季节对乌龙茶品质影响的研究[J]. 茶叶科学, 2002(2): 135-139, 146.
[10] 金珊, 余有本, 张秀云, 等. 设施栽培对绿茶品质的影响[J]. 中国农学通报, 2009(15): 261-267.
[11] 黄意欢. 茶学实验技术[M]. 北京: 中国农业出版社,1997: 115.
[12] 施兆鹏. 茶叶加工学[M]. 北京: 中国农业出版社, 1997.
[13] 张梁, 周杰, 秦金花. 多种茶叶废料中多酚类成分和咖啡碱的含量测定[J]. 食品科学, 2013(20): 113-116.
[14] 钟萝. 茶叶品质理化分析[M]. 上海: 上海科学技术出版社, 1989: 453-454.
[15] 陈默, 王志伟, 胡长鹰, 等. 酶标仪法快速评价香兰素的抑菌活性[J]. 食品与发酵工业, 2009(5): 63-66.
[16] 张赟彬, 刘笑宇, 姜萍萍, 等. 肉桂醛对大肠杆菌和金黄色葡萄球菌的抑菌作用及抑菌机理研究[J]. 现代食品科技, 2015(5): 31-35.
[17] 杨联松, 檀根甲, 徐美清. 茶多酚抑菌作用和防腐效果初探[J]. 安徽农业科学, 1996(4): 86-88.
[18] 王丽, 许奇, 徐顺, 等. 茶多酚对微生物生长影响的研究进展[J]. 现代食品科技, 2013(7): 1 737-1 741.
[19] XIONG Li-gui, CHEN Yi-jun, TONG Jie-wen, et al. Tea polyphenol epigallocatechin gallete inhibits Escherichia coli by increasing endogenous oxidative stress[J]. Food Chemistry, 2017, 217: 196-204.
[20] 曾红亮, 黄灿灿, 常青, 等. 金柑多糖微波辅助提取工艺优化及抑菌效果[J]. 食品与机械, 2016, 32(9): 154-160.
[21] 高洁, 董文宾, 王勇, 等. 山皂荚多糖的提取工艺及抑菌活性[J]. 食品与机械, 2017, 33(3): 145-149, 206.