Abstract
A high sensitivity fluorescence detection method for ochratoxin A (OTA) based on OTA aptamers and gold nanoparticle (AuNPs) was established, which employed OTA aptamers as recognition element and AuNPs to quench the fluorescence emission from fluorescein. The results indicated that the detection limit is 10 nmol/L and a linear relationship between the fluorescent intensity ratio and the logarithm of OTA concentration was plotted in the range of 25～1 000 nmol/L (R2=0.991). The detection method showed high feasibility and reliability. Compared with traditional method, this method greatly improved the detection efficiency and provided technical support for more effective identification of OTA.
Publication Date
7-28-2017
First Page
56
Last Page
60
DOI
10.13652/j.issn.1003-5788.2017.07.012
Recommended Citation
Lei, LU; Yangyang, ZHAO; Renjie, LIU; Yongdong, JIN; and Zhijun, GUO
(2017)
"Study on detection method for ochratoxin A based on gold nanoparticle and aptamer,"
Food and Machinery: Vol. 33:
Iss.
7, Article 12.
DOI: 10.13652/j.issn.1003-5788.2017.07.012
Available at:
https://www.ifoodmm.cn/journal/vol33/iss7/12
References
[1] AHMED N E, FARAG M M, SOLIMAN K M, et al. Evaluation of methods used to determine ochratoxin a in coffee beans[J]. Journal of Agricultural and Food Chemistry, 2007, 55(23): 9 576-9 580.
[2] BREITHOLTZ A, OLSEN M, DAHLBCK , et al. Plasma ochratoxin A levels in three Swedish populations surveyed using an ion-pair HPLC technique[J]. Food Additives & Contaminants, 1991, 8(2): 183-192.
[3] OLIVEIRA S C B, DICULESCU V C, PALLESCHI G, et al. Electrochemical oxidation of ochratoxin A at a glassy carbon electrode and in situ evaluation of the interaction with deoxyribonucleic acid using an electrochemical deoxyribonucleic acid-biosensor[J]. Analytica Chimica Acta, 2007, 588(2): 283-291.
[4] IMPERATO R, CAMPONE L, PICCINELLIA L, et al. Survey of aflatoxins and ochratoxin a contamination in food products imported in Italy[J]. Food Control, 2011, 22(12): 1 905-1 910.
[5] LI Zeng-ning, ZHANG Xiang-hong, CUI Jin-feng, et al. Assessment on pollution of ochratoxin a in grain in China and its apoptosis effect on vitro-cultured human tubular kidney cells[J]. Journal of Biochemical and Molecular Toxicology, 2012, 26(4): 139-146.
[6] RACHED E, HOFFMANN D, BLUMBACH K, et al. Evalu-ation of putative biomarkers of nephrotoxicity after exposure to ochratoxin A In vivo and In vitro[J]. Toxicological Sciences, 2008, 103(2): 371-381.
[7] ROBINSON A L, LEE H J, RYU D. Polyvinylpolypyrrolidone reduces cross-reactions between antibodies and phenolic compounds in an enzyme-linked immunosorbent assay for the detection of ochratoxin A[J]. Food Chemistry, 2017, 214: 47-52.
[8] DUARTE S C, PENA A, LINO C M. A review on ochratoxin A occurrence and effects of processing of cereal and cereal derived food products[J]. Food Microbiology, 2010, 27(2): 187-198.
[9] LUHE A, HILDEBRAND H, BACH U, et al. A new approach to studying ochratoxin A (OTA)-induced nephrotoxicity: Expression profiling in vivo and in vitro employing cDNA microarrays[J]. Toxicological Sciences, 2003, 73(2): 315-328.
[10] 刘仁荣, 徐玲, 裘雪梅, 等. 基于赭曲霉毒素A模拟表位的无毒素ELISA方法[J]. 食品与机械, 2010, 26(6): 47-50.
[11] CIGIC I K, STRLIC M, SCHREIBER A, et al. Ochratoxin A in wine: Its determination and photostability[J]. Analytical Letters, 2006, 39(7): 1 475-1 488.
[12] TURNER N W, SUBRAHMANYAM S, PILETSKY S A. Analytical methods for determination of mycotoxins: A review[J]. Analytica Chimica Acta, 2009, 632(2): 168-180.
[13] BECERRA V, ODERMATT J. Detection and quantification of traces of bisphenol A and bisphenol S in paper samples using analytical pyrolysis-GC/MS[J]. Analyst, 2012, 137(9): 2 250-2 259.
[14] ROLAND A, BROS P, BOUISSEAU A, et al. Analysis of ochratoxin A in grapes, musts and wines by LC-MS/MS: First comparison of stable isotope dilution assay and diastereomeric dilution assay methods[J]. Analytica Chimica Acta, 2014, 818(25): 39-45.
[15] 傅武胜, 邱文倩, 郑奎城, 等. 药食两用类食品中赭曲霉毒素A的高效液相色谱—荧光检测方法[J]. 食品科学, 2011, 32(14): 298-302.
[16] ELLINGTON A D, SZOSTAK J W. Invitro selection of RNA molecules that bind specific ligands[J]. Nature, 1990, 346(6 287): 818-822.
[17] TUERK C, GOLD L. Systematic evolution of ligands by exponential enrichment-RNA ligands to bacteriophage-T4 DNA-polymerase[J]. Science, 1990, 249(4 968): 505-510.
[18] 吴世嘉, 聂雨, 张辉, 等. 基于KGdF4: Dy3+纳米材料检测土霉素的生物传感新方法[J]. 食品与机械, 2014, 30（6）： 64-68.
[19] HE Yu, YE Tao, SU Min, et al. Hierarchical self-assembly of DNA into symmetric supramolecular polyhedra[J]. Nature, 2008, 452(7 184): 198-201.
[20] CHEN Yu-wen, SASIREKHA N. Preparation of NiFeB Nanoalloy Catalysts and Their Applications in Liquid-Phase Hydrogenation of p-Chloronitrobenzene[J]. Industrial & Engineering Chemistry Research, 2009, 48(13): 6 248-6 255.
[21] NIU Xin-shu, LI Hong-hua, LIU Guo-guang. Preparation, characterization and photocatalytic properties of REFeO3 (RE=Sm, Eu, Gd)[J]. Journal of Molecular Catalysis A: Chemical, 2005, 232(1): 89-93.
[22] SHAO Xin, XUE Qun-ji, LIU Wei-min, et al. Tribological behavior of micrometer- and nanometer-Al2O3-particle-filled poly(phthalazine ether sulfone ketone) copolymer composites used as frictional materials[J]. Journal of Applied Polymer Science, 2005, 95(5): 993-1 001.
[23] HALLETT-TAPLEY G L, D'ALFONSO C, PACIONIN L, et al. Gold nanoparticle catalysis of the cis-trans isomerization of azobenzene[J]. Chemical Communications, 2013, 49(86): 10 073-10 075.
[24] GUO Ya-hui, WANG Xiong-tao, MIAO Bin, et al. An AuNPs-functionalized AlGaN/GaN high electron mobility transistor sensor for ultrasensitive detection of TNT[J]. RSC Advances, 2015, 5(120): 98 724-98 729.
[25] PRADO-GOTOR R, GRUESO E, PEREZ-TEJEDA P, et al. Free energy of binding of cationic metal complexes to AuNPs through electron-transfer processes[J]. Soft Matter, 2014, 10(42): 8 482-8 488.
[26] LU Qiu-jun, ZHAO Jiang-na, XUE Shan-yan, et al. A "turn-on" fluorescent sensor for ultrasensitive detection of melamine based on a new fluorescence probe and AuNPs[J]. Analyst, 2015, 140(4): 1 155-1 160.
[27] TU Yun-qiu, WU Ping, ZHANG Hui, et al. Fluorescence quenching of gold nanoparticles integrating with a conformation-switched hairpin oligonucleotide probe for microRNA detection[J]. Chemical Communications, 2012, 48(87): 10 718-10 720.
[28] FRENS G. Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions[J]. Nature-Physical Science, 1973, 241(105): 20-22.
[29] GRABAR K C, FREEMAN R G, HOMMER M B, et al. Preparation and Characterization of Au Colloid Monolayers[J]. Analytical Chemistry, 1995, 67(4): 735-743.
[30] JIN Rong-chao, WU Guo-sheng, LI Zhi, et al. What Controls the Melting Properties of DNA-Linked Gold Nanoparticle Assemblies?[J]. Journal of the American Chemical Society, 2003, 125(6): 1 643-1 654.