Study on inhibition kinetics of peroxidase from Lonicera Japonica Thunb

Authors

LUO Lei, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan 471023, China; Henan Agricultural Products Drying Equipment Engineering Technology Research Center, Luoyang, Henan 471023, China
DONG Jinlong, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan 471023, China; Henan Agricultural Products Drying Equipment Engineering Technology Research Center, Luoyang, Henan 471023, China
ZHU Wenxue, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan 471023, China; Henan Agricultural Products Drying Equipment Engineering Technology Research Center, Luoyang, Henan 471023, China
XUE Yihan, College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan 471023, China; Henan Agricultural Products Drying Equipment Engineering Technology Research Center, Luoyang, Henan 471023, China

Abstract

In order to purify honeysuckle peroxides and study the enzymatic properties and inhibition kinetics,DEAE cellulose DE-52 ion exchange chromatography was used for purification of peroxidase from Lonicera Japonica Thunb prepared preliminarily by three-phase partitioning. Two kinds of peroxidase, HSPⅠ（honeysuckle peroxidaseⅠ）and HSPⅡ（honeysuckle peroxidaseⅡ）, were purified,the specific activity were 3 312.3 U/mg and 564.8 U/mg respectively. The double substrate enzymatic reaction type was PingPong reaction analyzed by the reaction kinetics. Citric acid, Sodium metabisulfite showed irreversible inhibition to Lonicera Japonica Thunb peroxidase, L-cysteine showed reversible inhibition, and L-cysteine was a competitive inhibitor that the inhibition constant K_I was 0.053 mmol/L.

Publication Date

7-28-2017

First Page

111

Last Page

116

DOI

10.13652/j.issn.1003-5788.2017.07.026

Recommended Citation

Lei, LUO; Jinlong, DONG; Wenxue, ZHU; and Yihan, XUE (2017) "Study on inhibition kinetics of peroxidase from Lonicera Japonica Thunb," Food and Machinery: Vol. 33: Iss. 7, Article 26.
DOI: 10.13652/j.issn.1003-5788.2017.07.026
Available at: https://www.ifoodmm.cn/journal/vol33/iss7/26

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