Extraction of polysaccharide from germinated brown rice by aqueous two-phase system and research of its antioxidant activity

Authors

LIU Xiaofei, Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin, Heilongjiang 150086, China; Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin, Heilongjiang 150076, China
WANG Xin, Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin, Heilongjiang 150076, China
MENG Qinghong, Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin, Heilongjiang 150086, China
ZHANG Yu, Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin, Heilongjiang 150076, China
GONG Lili, Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin, Heilongjiang 150076, China
MA Yongqiang, Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin, Heilongjiang 150076, China
LU Shuwen, Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin, Heilongjiang 150086, China

Abstract

The polysaccharide was extracted by the ultrasonic-assisted aqueous two-phase technology from germinated brown rice. The phase separation ability of different in PEG/(NH₄)₂SO₄ system was studied according to the polysaccharide yield. The yield of polysaccharide substance of germinated brown rice was determined by orthogonal experiment. The optimum process conditions were determined. Results were showed as follows: the mass fraction of PEG6000 was 15.7%, the mass fraction of ammonium sulfate was 14.8% and extraction time was 40 min. The yield of polysaccharide substance from germinated brown rice was 81.07% in the optimum conditions. Morever, the anti-oxidation of polysaccharide substance from germinated brown rice were studied by methods of DPPH, pyrogallol autoxidation and phenanthroline. The results showed that the polysaccharide of brown rice had stronger antioxidant effect to DPPH, ultra oxygen anion and hydroxyl radicals.

Publication Date

7-28-2017

First Page

149

Last Page

154

DOI

10.13652/j.issn.1003-5788.2017.07.033

Recommended Citation

Xiaofei, LIU; Xin, WANG; Qinghong, MENG; Yu, ZHANG; Lili, GONG; Yongqiang, MA; and Shuwen, LU (2017) "Extraction of polysaccharide from germinated brown rice by aqueous two-phase system and research of its antioxidant activity," Food and Machinery: Vol. 33: Iss. 7, Article 33.
DOI: 10.13652/j.issn.1003-5788.2017.07.033
Available at: https://www.ifoodmm.cn/journal/vol33/iss7/33

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