Abstract
40 strains of actic acid bacteria isolated by traditional pickled cabbage were regarded as the object. Through the tests of acid resistance, anti gastric juice and bile salt resistance, actic acid bacteria with good resistance in traditional pickles were determined, using 16S rDNA sequence and phylogenetic tree to analyze the genus relationship of good resistance of lactic acid bacteria. The experimental results showed that MFR-28 and MFR-30 facing to artificial gastric juice and bile salt had a strong tolerance in 40 strains of actic acid bacteria, and survival rates in the artificial gastric juice were 85.21% and 90.47% respectively, and the growth of efficiency in 0.3% bile salt were 11.84% and 7.37% respectively; through the analysis of 16S rDNA sequence and phylogenetic tree, MFR-28 and MFR-30 were Lactobacillus casei. In this study, 2 strains of resistant lactobacillus casei can be used as potential probiotics to further explore their physiological functions and to develop probiotic products.
Publication Date
7-28-2017
First Page
26
Last Page
30
DOI
10.13652/j.issn.1003-5788.2017.07.006
Recommended Citation
Siyu, LIU; Jing, XIAO; and Huayi, SUO
(2017)
"Screening and identification of resistant lactobacillus in traditional pickles,"
Food and Machinery: Vol. 33:
Iss.
7, Article 6.
DOI: 10.13652/j.issn.1003-5788.2017.07.006
Available at:
https://www.ifoodmm.cn/journal/vol33/iss7/6
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