Study on total polyphenol content and antioxidant, antimicrobial activities of different polarity fractions of Chimonanthus salicifolicus Hu leaves

Authors

WU Yongxiang, College of Life and Environment Science, Huangshan University, Huangshan, Anhui 245041, China
WANG Xiang, College of Life and Environment Science, Huangshan University, Huangshan, Anhui 245041, China
JIANG Haitao, College of Life and Environment Science, Huangshan University, Huangshan, Anhui 245041, China
DING Kangjun, College of Life and Environment Science, Huangshan University, Huangshan, Anhui 245041, China
ZHAO Chenglong, College of Life and Environment Science, Huangshan University, Huangshan, Anhui 245041, China
HU Changyu, College of Life and Environment Science, Huangshan University, Huangshan, Anhui 245041, China

Abstract

Chimonanthus salicifolicus Hu leaves extracts were prepared by methanol circumfluence extraction. Five fractions were obtained by extraction with different polar solvents including n-hexane, dichloromethane, ethyl acetate, n-butanol and water. Then fractions were detected by total polyphenol content, reducing power, scavenging DPPH free radical and antimicrobial activities. The results showed that all the fractions exhibited high content of total polyphenol, and ethyl acetate fraction had the highest phenolic compounds, with the content of (309.50±4.81) mg/g. The fractions had strong antioxidative capacities such as reducing power and DPPH free radical scavenging ability. The fractions of methanol, n-hexane, dichloromethane, ethyl acetate, n-butanol and water had certain scavenging ability on DPPH free radical with IC₅₀ of 1.24, 1.11, 0.61, 0.48, 0.98 and 1.36 mg/mL, respectively. The fractions also had the inhibiting effects on E. coli, S.aureus, B.subtilis and P.aeruginosa. Dichloromethane fraction presented the best antimicrobial effect than other fractions. Its MIC against four common bacterial strains was 2 mg/mL. In conclusion, the fractions have good antioxidant and antimicrobial activities, and this research was profitable for the further study of the separation of active compounds from Chimonanthus salicifolicus Hu leaves.

Publication Date

8-28-2017

First Page

150

Last Page

154

DOI

10.13652/j.issn.1003-5788.2017.08.033

Recommended Citation

Yongxiang, WU; Xiang, WANG; Haitao, JIANG; Kangjun, DING; Chenglong, ZHAO; and Changyu, HU (2017) "Study on total polyphenol content and antioxidant, antimicrobial activities of different polarity fractions of Chimonanthus salicifolicus Hu leaves," Food and Machinery: Vol. 33: Iss. 8, Article 33.
DOI: 10.13652/j.issn.1003-5788.2017.08.033
Available at: https://www.ifoodmm.cn/journal/vol33/iss8/33

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