Breeding of low-permeability sensitive mutant from Saccharomyces cerevisiae

Authors

YE Jing, School of Chemical & Biological Engineering, Changsha University of Science & Technology, Changsha, Hunan 410004, China
YUE Xijie, College of Biological & Information Engineering, Yiyang Vocationa & Technicall College, Yiyang, Hunan 410600, China
JIANG Xuewei, School of Chemical & Biological Engineering, Changsha University of Science & Technology, Changsha, Hunan 410004, China; Guangdong Yixianmei BIO-SCI Co., Ltd, Qingyuan, Guangdong 513300, China
LUO Xiaoming, School of Chemical & Biological Engineering, Changsha University of Science & Technology, Changsha, Hunan 410004, China
CHEN Daiwen, Guangdong Yixianmei BIO-SCI Co., Ltd, Qingyuan, Guangdong 513300, China
WU Jian, School of Chemical & Biological Engineering, Changsha University of Science & Technology, Changsha, Hunan 410004, China
LI Chiling, School of Chemical & Biological Engineering, Changsha University of Science & Technology, Changsha, Hunan 410004, China

Abstract

In order to improve the efficient secretion of intracellular product in Saccharomyces cerevisiae, wild diploid Y₁ (2n) were mutagenized with UV treatment as original strain. To further identify, the degree of autolysis of each mutant strain was contrasted in low permeability condition by the test of its content of FDP, extra-cellular nucleic acids and proteins. The results showed: Hs₅*（n）showed the highest degree of conditional autolysis in low permeability condition, the concentration of FDP in extracellular environment was 25.81 μg/mL. At the sixth hour in low permeability, its penetration rate of nucleic acid and protein in extracellular environment were 1.86 and 2.07, respectively. In addition, mutant strain Hs₂*（n）, Hs₁（2n）also had a great extent of conditional autolysis capacity. These three low-permeability mutant strains obtained in this study possessed greater efficiency of intracellular product secretion contrast with wild strain.

Publication Date

8-28-2017

First Page

23

Last Page

26,154

DOI

10.13652/j.issn.1003-5788.2017.08.006

Recommended Citation

Jing, YE; Xijie, YUE; Xuewei, JIANG; Xiaoming, LUO; Daiwen, CHEN; Jian, WU; and Chiling, LI (2017) "Breeding of low-permeability sensitive mutant from Saccharomyces cerevisiae," Food and Machinery: Vol. 33: Iss. 8, Article 6.
DOI: 10.13652/j.issn.1003-5788.2017.08.006
Available at: https://www.ifoodmm.cn/journal/vol33/iss8/6

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