Isolation and identification of ginkolides-producing endophytic fungi from Ginkgo biloba Linn.

Authors

CHEN Xiaoming, College of Chemistry and Bioengineering in Hunan University of Science and Engineering, Yongzhou, Hunan 425199, China; Hunan Provincial Engineering Research Center for Ginkgo Biloba, Yongzhou, Hunan 425199, China; Key Laboratory of Comprehensive Utilizati
HE Fulin, College of Chemistry and Bioengineering in Hunan University of Science and Engineering, Yongzhou, Hunan 425199, China; Hunan Provincial Engineering Research Center for Ginkgo Biloba, Yongzhou, Hunan 425199, China; Key Laboratory of Comprehensive Utilizati
LI Zhizhong, Yongzhou Bureau of Animal Husbandry and Fishery, Yongzhou, Hunan 425199, China
YUAN Zhihui, College of Chemistry and Bioengineering in Hunan University of Science and Engineering, Yongzhou, Hunan 425199, China; Hunan Provincial Engineering Research Center for Ginkgo Biloba, Yongzhou, Hunan 425199, China; Key Laboratory of Comprehensive Utilizati

Abstract

In the present study, the method of tissue culturing was used to isolate the endophytic fungi from healthy ginkgo roots and stems. After repeated separation and purification, 116 Ginkgo endophytes were isolated. Metabolites of these fungi were preliminarily analyzed by high-performance liquid chromatography (HPLC), and a ginkgolide producing endophyte strain was finally screened out. The production of ginkgolides by this strain was more than 11.4 mg/L. The endophytic fungi strain was identified as Mucor spp. by morphology characteristics and phylogenetic analysis of internal transcribed spacer (ITS), and It was the first report in the ginkgolides producing endophytic fungus from Ginkgo biloba.

Publication Date

8-28-2017

First Page

27

Last Page

30

DOI

10.13652/j.issn.1003-5788.2017.08.007

Recommended Citation

Xiaoming, CHEN; Fulin, HE; Zhizhong, LI; and Zhihui, YUAN (2017) "Isolation and identification of ginkolides-producing endophytic fungi from Ginkgo biloba Linn.," Food and Machinery: Vol. 33: Iss. 8, Article 7.
DOI: 10.13652/j.issn.1003-5788.2017.08.007
Available at: https://www.ifoodmm.cn/journal/vol33/iss8/7

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