Abstract
Extractions of flavonoid compounds in onion with ethanol, using the optimization factors of single factor and orthogonal design experiments, the flavonoids extraction rate of onion peel was 21.58%. The effect of quercetin on the differentiation of 3T3-L1 preadipocytes was best by separating the flavonoids from the liquid phase and acting on the 3T3-L1 preadipocytes. The expression of fat differentiation related gene FASmRNA was measured in 3T3-L1 adipocytes induced by flavonoid monomers of onion skin for eighth days. The results showed that the relative expression of FAS in 3T3-L1 preadipocytes induced by mRNA in quercetin group was significantly lower than that in control group after 8D.
Publication Date
8-28-2017
First Page
31
Last Page
33,43
DOI
10.13652/j.issn.1003-5788.2017.08.008
Recommended Citation
Ying, HAN; Mo, LIN; Haoming, LIU; Meina, XU; Hongyan, XU; and Guanhao, LI
(2017)
"Study on the inhibitory effect of onion peel flavonoids on adipocyte production,"
Food and Machinery: Vol. 33:
Iss.
8, Article 8.
DOI: 10.13652/j.issn.1003-5788.2017.08.008
Available at:
https://www.ifoodmm.cn/journal/vol33/iss8/8
References
[1] 周长久. 蔬菜种质资源概论[M]. 北京: 北京农业大学出版社, 1995: 98-113.
[2] SMITH E A, SHIELDS E J, NAULT B A. Onion thrips colonization of onion fields bordering crop and non-crop habitats in muck cropping systems[J]. Journal of Applied Entomology, 2017, 141(7): 574-582.
[3] CHOI S M, LEE D J, KIM J Y, et al. Volatile composition and sensory characteristics of onion powders prepared by convective drying[J]. Food Chemistry, 2017, 231(15): 386-392.
[4] 冯长根, 吴悟贤, 刘霞, 等. 洋葱的化学成分及药理作用研究进展[J]. 上海中医药杂志, 2003, 37(7): 63-64.
[5] 付学军. 洋葱功能成分及其应用研究[D]. 济南: 山东大学, 2006: 8-14.
[6] CHEN J H, CHEN H I, WANG J S, et al. Effects of welsh onion extracts on human platelet function in vitro.[J]. Life Sciences, 2000, 66(17): 1 571-1 579.
[7] 刘昊明, 魏丹, 等. 洋葱皮提取物对营养性肥胖大鼠的减肥作用[J]. 食品与机械, 2015, 31(6): 160-163.
[8] AUGUSTI K T, ARATHY S L, ASHA R. Acomporative study on the beneficizl effets of garlic, amla and oiononr the hyper lipidemia induced by butter fat and beef fat inrats [J]. Experimental Biology, 2001, 39(8): 760-766.
[9] FANG Wei, RUAN Jin-lan, WANG Zhong, et al. Acetylated flavanone glycosides from the rhizomes of Cyclosorus acuminatus[J]. Nat ural Products, 2006, 69(11): 1 641-1 644.
[10] 隋苗苗. 洋葱皮黄酮类化合物对C57BL/6J小鼠抗肥胖作用的研究[D]. 延吉: 延边大学, 2014.
[11] 薛冬娜. 荷叶总黄酮提取纯化工艺及减肥降脂作用的研究[D]. 西安: 西南大学, 2008: 33-38.
[12] 杨俊朋. GLP-1对3T3-L1前脂肪细胞分化的影响及其调控机制研究[D]. 济南: 山东大学, 2013: 15-17.
[13] 迟玉杨. LiCl对3T3-L1前脂肪细胞分化以及Villin2和β-catenin 基因表达的影响[D]. 长春: 吉林大学, 2016: 10-12.
[14] 蒋益虹. 荷叶黄酮的乙醇提取工艺优化研究[J]. 农业工程学报, 2004, 20(4): 168-171.
[15] 陈勇. 荷叶黄酮提取、分离纯化及其抗氧化活性的研究[D]. 芜湖: 安徽工程大学, 2006: 26-27.
[16] 陆叶, 陈桢, 肖旭, 等. 高效液相色谱法测定秋鼠麴草中总黄酮的含量[J]. 苏州大学学报: 医学版, 2012, 32(1): 81-83.
[17] 吴亚林, 李小平, 傅求平, 等. RP-HPLC测定委陵菜中槲皮素含量研究[J]. 园艺与种苗, 2013(2): 4-5.