Abstract
The research was done in order to evaluate the effect of D-3-Phenyllactic acid (D-PLA) on preservation of grape and provide reference for SO2-free storage of table grapes. ‘Kyoho’ grapes were treated through post-harvest dipping before room temperature storage so that inhibitory potentials of microbial population on fruit surface were evaluated avoiding the fruit decay . The results showed that compared with the two control groups of purified water soakage and coating treatment, the D-PLA combined with coating treatment could effectively inhibit the growth of microorganism on fruit surfaces. It was found that the bacterial population were reduced by 99% and 99.3% respectively(P<0.05), and the mould population were reduced by 75% and 94.17% respectively (P<0.05). Moreover, the yeast population were found reduced by 98.13% and 97.45% respectively(P<0.05), and the coliform population on fruit surfaces of the D-PLA combined with coating treatment were also suppressed. Consquently, the fruit decay rate of the D-PLA combined with coating treatment was reduced by 20.65% and 23.68%(P<0.05)at the end of room temperature storage. These findings indicated the D-PLA combined with coating treatment was much better than individual coating treatment on antisepsis and the fresh-keeping of the grape, and D-PLA has a great application potential in its biological preservation field.
Publication Date
1-28-2018
First Page
117
Last Page
120
DOI
10.13652/j.issn.1003-5788.2018.01.023
Recommended Citation
Yulian, WAN; Liushun, CHEN; Dan, XIE; Yibo, ZHU; Dongxing, ZHU; and Bin, QI
(2018)
"The effect of D-phenyllactic acid combined with coating treatment on anti-microbial and preservation of grapes during storage,"
Food and Machinery: Vol. 34:
Iss.
1, Article 23.
DOI: 10.13652/j.issn.1003-5788.2018.01.023
Available at:
https://www.ifoodmm.cn/journal/vol34/iss1/23
References
[1] 程璐, 缪铭, 张涛, 等. 食品生物防腐剂——抗真菌乳酸菌研究进展[J]. 食品与发酵工业, 2010, 36(9): 129-133.
[2] DIEULEVEUX V, LEMARINIER S, GUEGUEN M. Antimicro-bial spectrum and target site of D-3-phenyllactic acid[J]. International Journal of Food Microbiology, 1998, 40(3): 177-183.
[3] DIEULEVEUX V, GUEGUEN M. Antimicrobial effeets of D-3-Phenyllactic acid on Listeria monocytogenes in TSB-YE medium, milk, and cheese[J]. Journal of Food Protection, 1998, 61(10): 1 281-1 285.
[4] 蒋卓越, 季伟, 钱蓓蓓, 等. 乳酸脱氢酶突变体 D-LDHY52L 在大肠杆菌中的重组表达及其合成 D-苯基乳酸的研究[J]. 食品与发酵工业, 2016, 42(1): 1-6.
[5] STORM K, SCHNURER J, MELIN P. Co-cultivation of antifungal Lactobacillus Plantarum MiLAB 393 and AsPergillus nidulans, evaluation of effects on fungal growth and protein expression[J]. FEMS Microbial Letters, 2005, 246(1): 119-124.
[6] LAVERMICOCCA P, VALERIO F, VIACONTI A. Antifungal activity of phenyllactic acid against molds isolated from bakery products[J]. Applied & Environmental Microbiology, 2003, 69(1): 634-640.
[7] SCHWENNINGER S M, LACROIX C, TRUTTMANN S, et al. Characterization of low-molecular-weight antiyeast metabolites produced by a food-protective Lactobacillus-Propionibacterium coculture[J]. Journal of Food Protection, 2008, 71(12): 2 481-2 487.
[8] OHHIRA I, KUWAKI S, MORITA H, et al. Identification of 3-phenyllactic acid as a possible antibacterial substance produced by Enterococcus faecalisTH10[J]. Biocontrol Science, 2004, 9(3): 77-81.
[9] 李兴峰, 江波, 潘蓓蕾, 等. 新型生物防腐剂—苯乳酸在食品中的研究与应用[J]. 食品与发酵工业, 2007, 33(5): 87-91.
[10] VALERIO F, LAVERMICOCCA P, PASCALE M, et al. Production of phenyllactic acid by lactic acid bacteria: an approach to the selection of strains contributing to food quality and preservation[J]. FEMS Microbiology Letters, 2004, 233(2): 289-295.
[11] 芦夏霏, 刘毕琴, 柳陈坚, 等. 乳酸菌苯乳酸的合成及其代谢调控机制研究进展[J]. 食品与发酵工业, 2014, 40(11): 177-181.
[12] OBERDOERSTER J, GUIZZETTI M, COSTA L G. Effect of phenylalanine and its metabolites on the proliferation and viability of neuronal and astroglial cells: possible relevance in maternal phenylketonuria[J]. Journal of Pharmacology & Experimental Therapeutics, 2000, 295(1): 295-301.
[13] 朱益波, 蒋卓越, 郭倩, 等. 重组融合蛋白大肠杆菌不对称合成(R)-2-羟基-3-苯基丙酸[J]. 食品与发酵工业, 2017, 43(1): 49-54.
[14] 王颖, 何禾, 胡发根. 响应面法优化重组大肠杆菌全细胞合成D-苯基乳酸[J]. 食品科学, 2016, 37(7): 88-92.
[15] RODRIGUEZ N J, SALGADO M S, DOMINGUEZ M, et al. Antimicrobial activity ofD-3-phenyllactic acid produced by fed-batch process against Salmonella enterica[J]. Food Control, 2012, 25(1): 274-284.
[16] 袁景环, 贡汉生, 孟祥晨. 苯乳酸的抗菌作用及其抗菌机理的初步研究[J]. 食品工业, 2009(5): 14-17.
[17] DIEULEVEUX V, VAN D P D C, GUEGUEN M. Purification and characterization of anti-Listeria compounds produced by Geotrichum candidum[J]. Dalton Transactions, 1998, 64(2): 800-803.
[18] LAVERMICOCCA P, VALERIO F, EVIDENTE A, et al. Purification and characterization of novel antifungal compounds from the sourdough Lactobacillus Plantarum strain 21B[J]. Applied & Environmental Microbiology, 2000, 66(9): 4 084-4 090.
[19] AXEL C, BROSNAN B, ZANNINI E, et al. Antifungal sourdough lactic acid bacteria as biopreservation tool in quinoa and rice bread[J]. International Journal of Food Microbiology, 2016, 239(12): 86-94.
[20] BUI K T, NGUYEN D L. Effect of pre-treatment by phenyllactic acid combined with food additives on quality of minimally processed litchi fruits[J]. Journal of Science and Technology, 2014, 52(5C): 334-339.
[21] BUI K T, NGUYEN D L. The study on production of phenyllactic acid by fermentation and its application in preservation of fresh cut pineapple and orange fruit[J]. Journal of Science and Technology, 2013, 51(6A): 283-289.
[22] 朱东兴, 杭夫梅, 李娜, 等. 臭氧在鲜食葡萄无硫贮藏技术中的防腐保鲜效果[J]. 中国食品添加剂, 2010(3): 199-202.
[23] 秦丹, 石雪晖, 林亲录, 等. 葡萄采后病害生物防治研究进展[J]. 食品与机械, 2007, 23(6): 142-144.
[24] ARTES H F, TOMAS B F A, ARTES F. Modified atmosphere packaging preserves quality of SO2-free 'Superior seedless' table grapes[J]. Postharvest Biology & Technology, 2006, 39(2): 146-154.
[25] 李燕, 戴佳锟, 马赛箭, 等. 天然食品防腐剂及其在鲜切果蔬中的应用研究进展[J]. 食品与机械, 2013, 29(5): 230-233.
[26] 陈学红, 郑永华, 杨震峰, 等. 高氧处理对草莓采后腐烂和品质的影响[J]. 农业工程学报, 2004, 20(5): 200-202.
[27] 钟秋平, 周文化, 傅力. 食品保藏原理[M]. 北京: 中国计量出版社, 2010: 4.