Active evaluation of DNA protection to oxidative damage and its possible mechanisms of vitexin in vitro

Authors

CHEN Ban, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China
LI Dahuan, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China
LI Xican, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China

Abstract

In order to study the DNA protection and possible mechanisms of vitexin, it was measured by using three spectrophotometric assays, including DNA protection, Fe²⁺-complexing, and Cu²⁺ reducing. In addition, its Fe²⁺-complexing reaction was also analyzed using UV-Vis spectra. The results showed that the IC₅₀ values of vitexin were (919.6±23.6), (9.3±1.2) and (421.7±29.2) μmol/L, respectively, for DNA protection assay, Fe²⁺-complexing assay, and Cu²⁺ reducing assay. When mixed with Fe²⁺, vitexin gave a UV-Vis peak at 554 nm \[molar absorptivity ε=2 680.74 L/(mol·cm)\]. In conclusion, vitexin can effectively protect DNA from ·OH induced damage. The protective mechanism may include electron-transfer (ET) and Fe²⁺-complexing, and its Fe²⁺-complexing site is possibly between 4-carbonly and 5-hydroxyl groups.

Publication Date

1-28-2018

First Page

130

Last Page

134

DOI

10.13652/j.issn.1003-5788.2018.01.026

Recommended Citation

Ban, CHEN; Dahuan, LI; and Xican, LI (2018) "Active evaluation of DNA protection to oxidative damage and its possible mechanisms of vitexin in vitro," Food and Machinery: Vol. 34: Iss. 1, Article 26.
DOI: 10.13652/j.issn.1003-5788.2018.01.026
Available at: https://www.ifoodmm.cn/journal/vol34/iss1/26

References

[1] CADET J, WAGNER J R. DNA base damage by reactive oxygen species, oxidizing agents, and UV radiation[J]. Cold Spring Harbor Perspectives in Biology, 2013, 5(2): a12 559.
[2] KULMS D, ZEISE E, PPPELMANN B, et al. DNA damage, death receptor activation and reactive oxygen species contribute to ultraviolet radiation-induced apoptosis in an essential and independent way[J]. Oncogene, 2002, 21(38): 5 844.
[3] KARKI K, PANDE D, NEGI R, et al. An Assessment of oxidative damage and non-enzymatic antioxidants status alteration in relation to disease progression in breast diseases[J]. Med Sci (Basel), 2016, 4(4): 17.
[4] NORISHADKAM M, ANDISHMAND S, ZAVAR REZA J, et al. Oxidative stress and DNA damage in the cord blood of preterm infants[J]. Mutat Res, 2017, 824: 20-24.
[5] LI Xi-can, CHEN Dong-feng, MAI Ying, et al. Concordance between antioxidant activities in vitro and chemical components of Radix Astragali (Huangqi)[J]. Natural Product Research, 2012, 26(11): 1 050-1 053.
[6] 王鑫波, 高敏, 童丽姣, 等. 山楂叶提取物中牡荆素鼠李糖苷的含量测定[J]. 中华中医药学刊, 2010, 28(10): 2 098-2 100.
[7] JURIKOVA T, SOCHOR J, ROP O, et al. Polyphenolic profile and biological activity of Chinese hawthorn (Crataegus pinnatifida BUNGE) fruits[J]. Molecules, 2012, 17(12): 14 490-14 509.
[8] KANG Jie, LI Zhi-min, WU Tong, et al. Anti-oxidant capacities of flavonoid compounds isolated from acai pulp (Euterpe oleracea Mart)[J]. Food Chemistry, 2010, 122(3): 610-617.
[9] ZHANG Xue, XU Dao-hua. Research progress of pharmacolog-ical action of vitexin[J]. China Medical Herald, 2013, 35: 41-44.
[10] NI Qin-xue, LIU Ying-kun, GONG Ling-xiao, et al. Active components in six kinds of ground bamboo leaves and their anti-oxidant activities[J]. Chinese Traditional & Herbal Drugs, 2011, 42(11): 2 317-2 321.
[11] LI Xi-can, MAI Wen-qiong, LI Wang, et al. A hydroxyl-scavenging assay based on DNA damage in vitro[J]. Analytical Biochemistry, 2013, 438(1): 29-31.
[12] JIANG Qian, LI Xi-can, TIAN Ya-ge, et al. Lyophilized aqueous extracts of Mori Fructus and Mori Ramulus protect mesenchymal stem cells from ·OH-treated damage: bioassay and antioxidant mechanism[J]. Bmc Complementary & Alternative Medicine, 2017, 17(1): 242-252.
[13] LI Xi-can, HU Qiu-ping, JIANG Shu-xia, et al. Flos Chrysanthemi Indici protects against hydroxyl-induced damages to DNA and MSCs via antioxidant mechanism[J]. Journal of Saudi Chemical Society, 2015, 19(4): 454-460.
[14] KIWI J, LOPEZ A, NADTOCHENKO V. Mechanism and kinetics of the ·OH-radical intervention during Fenton oxidation in the presence of a significant amount of radical Scavenger (Cl^-)[J]. Environmental Science & Technology, 2000, 34(11): 2 162-2 168.
[15] PERRON N R, BRUMAGHIM J L. A review of the antioxid-ant mechanisms of polyphenol compounds related to iron binding[J]. Cell Biochemistry & Biophysics, 2009, 53(2): 75-100.
[16] LIN Jing, LI Xi-can, LI Chen, et al. Protective effect against hydroxyl radical-induced DNA damage and antioxidant mechanism of [6]-gingerol: A Chemical Study[J]. Bulletin of the Korean Chemical Society, 2014, 35(6): 1 633-1 638.
[17] LI Xi-can, LIU Jing-jing, LIN Jing, et al. Protective effects of dihydromyricetin against ·OH-induced mesenchymal stem cells damage and mechanistic chemistry[J]. Molecules, 2016, 21(5): 604-616.
[18] LI Xi-can, MAI Wen-qiong, CHEN Dong-feng. Chemical study on protective effect against hydroxyl-induced DNA damage and antioxidant mechanism of myricitrin[J]. Journal of the Chinese Chemical Society, 2014, 61: 383-391.
[19] LI Xi-can, JIANG Qian, WANG Ting-ting, et al. Comparison of the antioxidant effects of quercitrin and isoquercitrin: understanding the role of the 6″-OH group [J]. Molecules, 2016, 21(9): 1 246-1 256.
[20] MARINOV N M. A detailed chemical kinetic model for high temperature ethanol oxidation[J]. International Journal of Chemical Kinetics, 2015, 31(3): 183-220.
[21] XIE Yu-lu, LI Xi-can, XU Jie-ying, et al. Two phenolic antioxidants in Suoyang, enhance viability of ·OH-damaged mesenchymal stem cells: comparison and mechanistic chemistry [J]. Chemistry Central Journal, 2017, 11(1): 84-94.
[22] WANG Ting-ting, ZENG He-ping, LI Xi-can, et al. In vitro studies on the antioxidant and protective effect of 2-substituted-8-hydroxyquinoline derivatives against H₂O₂-Induced oxidative stress in BMSCs[J]. Chemical Biological Drug Design, 2010, 75(2): 214-222.
[23] LI Xi-can, LU Han, LI Yun-rong, et al. Protective effect of sinapine against hydroxyl radical-induced damage to mesenchymal stem cells and possible mechanisms[J]. Chem. Pharm. Bull. 64, 319-325,
[24] WANG Guang-rong, LI Xi-can, ZENG He-ping. Synthesis, antioxidation activity of (E)-9-p-Tolyl-3-[2-(8-hydroxy-quinol-2-yl)vinyl]-carbazole and (E)-9-(p-Anisyl)-3-[2-(8-hydroxy-quinol-2-yl)vinyl]-carbazole and their induction proliferation of mesenchymal stem cells[J]. Act Chimica Sinica, 2009, 67(9): 974-982.
[25] CHEN Dong-feng, LI Xi-can, XU Zhi-wei, et al. Hexadecanoic acid from Buzhong Yiqi decoction induced proliferation of bone marrow mesenchymal stem cells [J]. Journal of Medicinal Food, 2010, 13(4): 967-970.
[26] LI Xi-can, HAN Wei-juan, MAI Wen-qiong, et al. Antioxidant activity and mechanism of tetrahydroamentoflavone in vitro[J]. Natural Product Communications, 2013, 8(6): 787-789.
[27] LI Xi-can, WEI Gang, WANG Xiao-zhen, et al. Targeting of the Shh pathway by atractylenolides promotes chondrogenic differentiation of mesenchymal stem cells[J]. Biological & Pharmaceutical Bulletin, 2012, 35(8): 1 328-1 335.