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Abstract

Chicken bone contains plenty of various nutrition, however the efficiency of direct use is relatively low. The enzymatic hydrolysis with protease can significantly increase the efficiency. In the present study, an enzymatic hydrolysis process on chicken bone paste with double-enzyme combination was developed. By the orthogonal test analysis, the optimal process parameters were as follows. The amounts of the neutral protease and Flavourzyme complex flavored enzyme was 0.75% and 1.50%, respectively, and the substrate concentration was 5%, hydrolyzed at was 50 ℃ and pH 8.5 for 2.5 h. Under these conditions, degree of hydrolysis (DH) and nitrogen recovery of the hydrolysates were 37.11% and 92.5%, respectively. The hydrolysate had no significant bitter taste or strange odor, with the significant increase of the amount of the free amino acids.

Publication Date

1-28-2018

First Page

205

Last Page

208

DOI

10.13652/j.issn.1003-5788.2018.01.041

References

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