Abstract
The flavonoids from stems and leaves of Physalis alkekengi ( PSF ) were refined by macroporous adsorption resin, and their antioxidant activities were evaluated in vitro and in vivo. The PSF were obtained using with AB-8 macroporous adsorption resin washed by 70% ethanol, and the purity was 39.16%. After refining, the scavenging activities of PSF on NO-2·and ABTS+· were 81.36% and 91.49%. The anti-oxidative capability in linoleic acid lipid overoxidation system was 85.26%. The refined PSF not only significantly increased thymus index and spleen index(P<0.05), but also increased the activity of T-AOC, GSH-Px and SOD in serum(P<0.05) and decreased the content of MDA in serum(P<0.01). The refined PSF showed strong antioxidant activity in vitro and in vivo.
Publication Date
10-28-2018
First Page
158
Last Page
162
DOI
10.13652/j.issn.1003-5788.2018.10.032
Recommended Citation
Qun, LI; Mingzhe, SUN; Wei, WU; Xiujuan, JIANG; and Baiying, CAO
(2018)
"Refining flavonoids from stems and leaves of Physalis alkekengi and their antioxident activity,"
Food and Machinery: Vol. 34:
Iss.
10, Article 32.
DOI: 10.13652/j.issn.1003-5788.2018.10.032
Available at:
https://www.ifoodmm.cn/journal/vol34/iss10/32
References
[1] 杨晓虹, 陈滴, 周晓平, 等. 酸浆果实无机元素和氨基酸的含量测定[J]. 人参研究, 2000, 12(2): 34-36.
[2] 徐伟, 李迎楠, 高雪清, 等. 复合酶对红菇娘果浆酶解条件的优化[J]. 食品科学, 2013, 34(18): 114-117.
[3] 葛玉, 段玉峰, 刘俊花, 等. 黑龙江酸浆果实及宿萼的成分分析[J]. 营养学报, 2006, 28(6): 529-530
[4] 许惠芳, 王艳丽, 郭瑜, 等. 酸浆果总皂苷提取工艺及其体外生物活性研究[J]. 食品工业, 2017, 38(11): 131-137.
[5] 周正辉, 焦连庆, 于敏, 等. 锦灯笼根茎挥发油化学成分GC-MS分析[J]. 特产开发, 2012, 34(1): 69-71.
[6] 舒尊鹏, 徐炳清, 刑娜, 等. 锦灯笼化学成分[J]. 中国实验方剂学, 2014, 20(21): 99-102.
[7] 谢瑶瑶. 酸浆茎叶化学成分的研究[D]. 沈阳: 沈阳药科大学, 2009: 4-12.
[8] ALI Ahmed F, JAHAN N. Optimization of culture condition, phytoconstituents and cytotoxicity of Xylaria hypoxylon (L. ) Grev[J]. Jahangirnagar University J. Biol. Sci., 2017, 6(2): 1-9.
[9] 贺怡琼, 于宏伟, 史宝胜, 等. 野生酸浆根茎叶提取物的抑菌活性及其稳定性研究[J]. 食品科技, 2016(4): 208-212.
[10] LIANG L F, KURTN T, MNDI A, et al. Structural, stereochemical, and bioactive studies of cembranoids from Chinese soft coral Sarcophyton trocheliophorum[J]. Tetrahedron, 2018, 74(15): 1 933-1 944.
[11] BIMAKR M, RAHMAN R A, TAIP F S, et al. Comparison of different extraction methods for the extraction of major bioactiveflavonoid compounds from spearmint(Mentha spicata L. ) leaves[J]. Food and Bioproducts Processing, 2011, 89(1): 67-72.
[12] 赵丹, 张秀玲. 酸浆宿萼黄酮提取工艺优化与自由基清除活性研究[J]. 食品科技, 2013(1): 232-236.
[13] 邱莉, 姜志虎, 刘红霞, 等. 酸浆宿萼的黄酮苷类化学成分[J]. 沈阳药科大学学报, 2007, 24(12): 744-747.
[14] 敬思群, 郑力, 苏俊. 酸浆宿萼总黄酮和多糖的提取及含量测定[J]. 食品研究与开发, 2008, 29(6): 82-84.
[15] 赵跃刚, 程东岩, 范艳君, 等. 酸浆不同药用部位中总黄酮的含量测定[J]. 时珍国医国药, 2010, 21(11): 2 781-2 782.
[16] 曹柏营, 戚颖欣, 姜秀娟, 等. 藤本豆豆荚总黄酮提取及抗氧化活性研究[J]. 食品研究与开发, 2016, 37(23): 56-60.
[17] 范艳丽, 张博, 李梓溢, 等. 红枣核总黄酮的提取工艺及抗氧化活性研究[J]. 食品研究与开发, 2017, 38(3): 95-100.
[18] 曹清明, 邬靖宇, 钟海雁, 等. 油茶叶中黄酮的超声辅助提取及其抗氧化活性研究[J]. 食品与机械, 2015, 31(3): 162-166.
[19] 汪光华, 唐树平, 彭名军, 等. 高良姜中4种黄酮化合物的体外抗氧化能力及抑菌活性研究[J]. 食品与机械, 2017, 33(5): 168-172.
[20] 吕雄文, 李俊, 邹宇宏, 等. 老鹰茶总黄酮降血糖作用的实验研究[J]. 中国中医药科技, 2008, 15(2): 119-121.
[21] 陈飞, 何先元, 周卯勤, 等. 超声辅助提取四齿四棱草中总黄酮及其抗氧化活性[J]. 天然产物研究与开发, 2016, 28(1): 96-101.
[22] 张洁. 石榴汁的抗氧化作用研究[D]. 济南: 山东大学, 2008: 26-28.
[23] 王苗苗, 韩飞, 李慕春. 柳花提取物总黄酮含量及抗氧化活性的研究[J]. 食品研究与开发, 2017, 38(16): 20-23.