Abstract
In this study, the seeds of Prunus humilis were used to extract amygdalin from the kernels by using ultrasound-assisted ethanol , and HPLC was used for detection. The amygdalin extraction rate was used as the detection index, and the ethanol concentration and the liquid-to-material ratio, extraction temperature, ultrasonic time and ultrasonic power were tested by single factor test, and then the relationships between each factor and extraction rate were analyzed. Thereafter, based on the two-level, five-factor experiment and response surface method, ultrasonic extraction process were optimized to obtain the extraction of Eucalyptus seeds. The optimum conditions of amygdalin included liquid to material ratio 13∶1 (mL/g), ethanol concentration 79%, extraction at 70 ℃, ultrasonic for 40 min, and ultrasonic power 192 W, and the final yield was 6.01%.
Publication Date
10-28-2018
First Page
163
Last Page
168
DOI
10.13652/j.issn.1003-5788.2018.10.033
Recommended Citation
Xin, ZHANG; Ruiling, LI; Xueru, SUN; Qiong, WU; and Xiaotong, WU
(2018)
"Optimization of Ultrasonic-assisted extraction of amygdalin from Prunus humilis seed,"
Food and Machinery: Vol. 34:
Iss.
10, Article 33.
DOI: 10.13652/j.issn.1003-5788.2018.10.033
Available at:
https://www.ifoodmm.cn/journal/vol34/iss10/33
References
[1] 温琢, 高润宏. 欧李的研究进展[J]. 林副产品, 2016(12): 51-53.
[2] 张美莉, 邓秋才, 杨海霞, 等. 内蒙古欧李果肉和果仁中营养成分分析[J]. 氨基酸和生物资源, 2007, 29(4): 18-20.
[3] 冯媛媛. 欧李营养成分分析及低糖欧李果脯的开发[D]. 保定: 河北农业大学, 2015: 8-21.
[4] 白东海. 欧李果实类黄酮物质提取、组分鉴定及抗氧化能力研究[D]. 太谷: 山西农业大学, 2015: 19-52.
[5] 叶丽琴. 欧李果肉芳香物质构成特点、种质和地域差异及动态变化的研究[D]. 北京: 北京中医药大学, 2017: 7-14.
[6] 田金强, 兰彦平, 朱克瑞, 等. 欧李仁综合利用关键技术研究[J]. 中国油脂, 2012, 37(2): 65-69.
[7] 李秀珍, 李学强, 卢素丽. 微波辅助索氏提取法提取欧李仁油的工艺参数优化[J]. 生物学通报, 2013, 48(6): 47-49.
[8] 高友君, 任清. 人工栽培欧李仁油的提取、成分分析及抗氧化性[J]. 食品科学, 2015, 36(4): 80-85.
[9] 张玲. 欧李仁多肽的制备及其功能活性的研究[D]. 太谷: 山西农业大学, 2014: 37-42.
[10] 张伟, 林彤, 江英桥. RRLC 法同时测定橘红痰咳颗粒中苦杏仁苷和柚皮苷[J]. 中成药, 2012, 34(5): 865-868.
[11] ZHOU Cun-shan, QIAN Li-chun, MA Hai-le, et al. Enhancement of amygdalin activated with β-D-glucosidase on HepG2 cells proliferation and apoptosis[J]. Carbohydrate Polymers, 2012, 90(1): 516-523.
[12] 孙卓琳, 刘京伟, 匡长春. 苦杏仁苷抑制人肺癌NCI-H1299细胞体外侵袭的机制[J]. 中国药科大学学报, 2016, 47(4): 479-482.
[13] MAKAREVIC J, TSAUR I, JUENGEL E, et al. Amygdalin delays cell cycle progression and blocks growth of prostate cancer cells in vitro[J]. Life Sciences, 2016, 147: 137-142.
[14] 郑巧玲, 郭义曹, 孙杰, 等. 苦杏仁苷促进人血T淋巴细胞早熟凝集染色体增殖的实验研究[J]. 环境与职业医学, 2009, 26(6): 572-574.
[15] 郭君其. 苦杏仁甙抑制成人肾脏成纤维细胞的增殖[J]. 中国组织工程研究与临床康复, 2008, 12(18): 3 575-3 578.
[16] DENG Jia-gang, LI Chun-yang, WANG Hai-lian, et al. Amygdalin mediates relieved atherosclerosis in apolipoprotein E deficient mice through the induction of regulatory T cells[J]. Biochemical and Biophysical Research Communications, 2011, 411(3): 523-529.
[17] ZHANG Xiang-qun, HU Jian-gong, ZHUO Yu-zhen, et al. Amygdalin improves microcirculatory disturbance and attenuates pancreatic fibrosis by regulating the expression of endothelin-1 and calcitonin gene-related peptide in rats[J]. Journal of the Chinese Medical Association, 2018, 81(5): 437-443.
[18] 赵静, 段杨峰, 孔繁渊, 等. 超声波辅助提取枇杷核苦杏仁苷工艺优化[J]. 食品科学, 2011, 32(8): 37-42.
[19] 邬晓勇, 孙雁霞, 何钢, 等. 欧李种仁中苦杏仁苷的提取及其抗氧化活性[J]. 湖北农业科学, 2013, 52(19): 4 764-4 767.
[20] 刘进波, 肖可, 张天其, 等. HPLC对比不同炮制方法对苦杏仁药材含量的影响[J]. 内蒙古中医药, 2014(20): 78.
[21] 谢朝晖, 李鑫, 李文博, 等. 超声波法提取山杏仁中苦杏仁苷的单因素试验研究[J]. 河南城建学院学报, 2012, 21(2): 31-37.
[22] YUE Kai, YE Meng, ZHOU Zu-ji, et al. The genus Cordyceps: a chemical and pharmacological review[J]. Journal of Pharmacy & Pharmacology, 2013, 65(4): 474-493.
[23] 王涛, 夏其乐, 陆胜民. 超声波提取杨梅疏果核仁中苦杏仁苷工艺研究[J]. 浙江农业学报, 2014, 26(3): 779-785.
[24] 马杰. 桃仁苦杏仁苷的提取及其抑菌活性研究[J]. 食品工业, 2015, 36(5): 116-120.
[25] 范贤哲, 李广凯, 蒋艳杰, 等. 苦杏仁中苦杏仁苷提取工艺研究[J]. 广州化工, 2018, 46(10): 81-84.