Abstract
In order to comprehensively utilize Flos Sophora resources and industrialized large-scale production, the development of optimal conditions for the purification of rutin by polyamide resins is highly desirable. Paraphlomis jauanica was used as the raw material, and four factors including concentration of sample solution, flow rate of the sample solution, ethanol concentration of the eluent and elution volume were investigated to ascertain its influence on the purification of rutin. When the concentration of the sample was 0.286 mg/mL, flow rate of the sample solution was 1.0 mL/min, with eluent of 80% ethanol and elution volume of 2.5 BV. Finally, the yield of rutin was 55.49% and the purity was 99.08%.
Publication Date
10-28-2018
First Page
169
Last Page
173
DOI
10.13652/j.issn.1003-5788.2018.10.034
Recommended Citation
Zhen, WANG and Yuan, QIN
(2018)
"Purification of Rutin from Flos Sophora,"
Food and Machinery: Vol. 34:
Iss.
10, Article 34.
DOI: 10.13652/j.issn.1003-5788.2018.10.034
Available at:
https://www.ifoodmm.cn/journal/vol34/iss10/34
References
[1] 国家药典委员会. 中华人民共和国药典: 一部[S]. 2015版. 北京: 中国医药科技出版社, 2015.
[2] 闫克玉, 高远翔. 大孔吸附树脂法纯化槐米总黄酮的研究[J]. 现代食品科技, 2009, 25(6): 596-599.
[3] 王婷, 王辉宪, 王玲, 等. 聚酰胺法分离、纯化罗望子壳黄酮的工艺研究[J]. 广州化工, 2014, 13(42): 93-97.
[4] MUHAMMAD U, MUHAMMAD A, BAHARULLAH K, et al. Antibiotic additive and synergistic action ofrutin, morin and quercetin against methicillin resistant Staphylococcus aureus[J]. BMC Complementary and Alternative Medicine, 2015, 15(1): 1-12.
[5] 李雪峰, 徐振秋, 刘俊, 等. 聚酰胺法纯化银杏总内酯的工艺研究[J]. 药学与临床研究, 2015, 23(4): 344-346.
[6] 涂瑶生, 施之琪. 槐米中芦丁提取纯化工艺研究[J]. 海峡药学, 2010, 22(1): 49-51.
[7] 冀德富, 程月召, 李慧峰, 等. 高纯度芦丁的制备实验研究[J]. 实验室科学, 2014, 17(4): 38-41.
[8] 雷燕妮, 张小斌. 乙醇回流法提取槐米中芦丁最佳条件探索[J]. 陕西农业科学, 2017, 63(8): 46-59.
[9] 舒俊翔, 王峥. 槐米中芦丁提取纯化工艺研究进展[J]. 湖南科技学院学院, 2016, 37(5): 44-46.
[10] 王峥, 曾凡荣, 舒俊翔, 等. 响应面分析法优化金槐总黄酮的提取工艺[J]. 广州化工, 2017, 45(20): 82-85.
[11] JIA Xin-bo, LIN Xin-jian, LIN Chen-qiang et al. Enhanced alkaline catalase production by Serratia marcescens FZSF01: Enzyme purification, characterization, and recombinant expression[J]. Electronic Journal of Biotechnology, 2017, 30(1): 110-117.
[12] 姚昕, 利舒文, 陈宗游, 等. 响应面法优化超声波辅助提取桂北金槐槐米中槲皮素工艺研究[J]. 时珍国医国药, 2017, 10(28): 2 401-2 405.
[13] 付起凤, 王德娟, 孟凡佳. 正交法优化槐米中芦丁的超声提取工艺[J]. 中医药信息, 2012, 29(6): 49-52.
[14] 罗亚东. 丙酮/(NH4)2SO4双水相体系分离纯化槐米中芦丁的研究[D]. 桂林: 广西大学, 2014: 63.
[15] 冯民昌, 田原, 贾海洋, 等. 高纯度芦丁树脂纯化工艺研究[J]. 化工时刊, 2015, 29(2): 10-18.
[16] 邱岚, 梁琍, 邱学云, 等. 响应面分析法优化槐米中芦丁提取工艺[J]. 食品研究与开发, 2018, 39(2): 97-102.
[17] MARIA L S, GERMAN E M, GERARDO A M, et al. Dicistroviridae: A new viral purification technique[J]. Revista Argentina de Microbiologia, 2017, 49(4): 311-314.
[18] 冯启蒙, 徐聪. 槐米中提芦丁纯化工艺研究[J]. 价值工程, 2012, 19(2): 307-308.
[19] 林春梅. 聚酰胺层析法分离纯化牛蒡叶中的总黄酮[J]. 食品与发酵工业, 2014, 40(2): 236-238.
[20] 陈菊. 中药槐米中芦丁提取工艺研究[J]. 中国医药科学, 2017, 7(15): 51-53.
[21] MOU Qian-qian, HE Jing-xia, YIN Rong-li, et al. Response surface optimized infrared-assisted extraction and UHPLC determination of flavonoid types from flos sophorae[J]. Molecules, 2017, 22(6): 1-12.
[22] 舒文将, 刘金磊, 邹蓉. 高效液相色谱法测定不同产地和采收期对槐米中芦丁含量的影响[J]. 时珍国医国药, 2017, 28(3): 709-711.
[23] IZA N Q, ARRUDAL V A P, RICARDO S. Application of chitosan matrix for delivery of rutin[J]. Original Paper, 2017, 14(3): 561-566.