Optimization of enzymatic transformation of2-O-α-D-glucopyranosyl-L-ascorbic acid

Authors

HUANG Liping, The Yellow Sea Fisheries Research Institute of Chinese Academy of Fishery Sciences, Key Laboratory of SustainableDevelopment of Polar Fishery, Ministry of Agriculture and Rural Affairs, Qingdao, Shandong 266071, China; Collegeof Food Science & Technology,
HAO Jianhua, The Yellow Sea Fisheries Research Institute of Chinese Academy of Fishery Sciences, Key Laboratory of SustainableDevelopment of Polar Fishery, Ministry of Agriculture and Rural Affairs, Qingdao, Shandong 266071, China; Jiangsu Collaborative InnovationCent
WANG Wei, The Yellow Sea Fisheries Research Institute of Chinese Academy of Fishery Sciences, Key Laboratory of SustainableDevelopment of Polar Fishery, Ministry of Agriculture and Rural Affairs, Qingdao, Shandong 266071, China
SUN Jingjing, The Yellow Sea Fisheries Research Institute of Chinese Academy of Fishery Sciences, Key Laboratory of SustainableDevelopment of Polar Fishery, Ministry of Agriculture and Rural Affairs, Qingdao, Shandong 266071, China
LIU Junzhong, The Yellow Sea Fisheries Research Institute of Chinese Academy of Fishery Sciences, Key Laboratory of SustainableDevelopment of Polar Fishery, Ministry of Agriculture and Rural Affairs, Qingdao, Shandong 266071, China

Abstract

2-O-α-D-glucopyranosyl L-ascorbic acid(AA-2G) was transformed by marine microorganism cyclodextrin glucanotrans-ferase (CGTase) using L-ascorbic acid and β-cyclodextrin(β-CD) as substrate. The qualitative and quantitative analyses of AA-2G were undertaken by LC-MS and HPLC. On the basis of single factor experiment Plackett-Burman design was carried out to determine three main effective factors (pH, substrate concentration, enzyme concentration). Then Box-Behnken design using three factors and three levels was applied to optimize synthesis conditions of AA-2G. The results indicated that the optimum conditions were pH 9.07, enzyme concentration 90.78 U/g·β-CD, substrate concentration 56.81 g/L, the ratio of substrate 1∶1(volume ratio), reacting for 24 h, at 45 ℃. Under this condition the yield of AA-2G was 10.62 g/L.

Publication Date

11-28-2018

First Page

183

Last Page

189

DOI

10.13652/j.issn.1003-5788.2018.11.037

Recommended Citation

Liping, HUANG; Jianhua, HAO; Wei, WANG; Jingjing, SUN; and Junzhong, LIU (2018) "Optimization of enzymatic transformation of2-O-α-D-glucopyranosyl-L-ascorbic acid," Food and Machinery: Vol. 34: Iss. 11, Article 36.
DOI: 10.13652/j.issn.1003-5788.2018.11.037
Available at: https://www.ifoodmm.cn/journal/vol34/iss11/36

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