Study on the transferring of the gene fragment from the genetically modified cottonseed hulls to the edible fungi

Authors

BAI Yalong, Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Science, Shanghai 201403, China
SUO Yujuan, Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Science, Shanghai 201403, China
LIN Ting, Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Science, Shanghai 201403, China
SHEN Yuanyuan, Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Science, Shanghai 201403, China
ZHAO Xiaoyan, Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Science, Shanghai 201403, China
LIU Haiyan, Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Science, Shanghai 201403, China
ZHOU Changyan, Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Science, Shanghai 201403, China

Abstract

It is a question in the field of food-safety that whether foreign genetically modified genes would transfer from the genetically modified cottonseed hulls to edible fungi. In this study, 15 Tremella fuciformis samples and 10 Pleurotus ostreatus samples (the cultivation media contained 75% and 92% genetically modified cottonseed hulls, respectively) collected from cultivation meida were evaluated by PCR to confirm whether the foreign genetically modified genes, CaMV 35S, NOS, CpTⅠ, Cry IA(c), were present. In addition, 47 samples which were probably grown in the media containing cottonseed hulls, were collected from the large and medium-sized supermarkets and farm produce markets. All of the results of PCR showed that the foreign sequences were not amplified by PCR. In conclusion, No exogenous transgenic fragments in the genetically modified cottonseed hulls was found transferred to the edible fungi.

Publication Date

2-28-2018

First Page

1

Last Page

5

DOI

10.13652/j.issn.1003-5788.2018.02.001

Recommended Citation

Yalong, BAI; Yujuan, SUO; Ting, LIN; Yuanyuan, SHEN; Xiaoyan, ZHAO; Haiyan, LIU; and Changyan, ZHOU (2018) "Study on the transferring of the gene fragment from the genetically modified cottonseed hulls to the edible fungi," Food and Machinery: Vol. 34: Iss. 2, Article 1.
DOI: 10.13652/j.issn.1003-5788.2018.02.001
Available at: https://www.ifoodmm.cn/journal/vol34/iss2/1

References

[1] 肖星凝, 袁娅, 廖霞, 等. 食用菌抗氧化活性成分及其抗氧化作用机制研究进展[J]. 食品与机械, 2016, 32(11): 207-211.
[2] 申进文, 刘超, 张倩, 等. 5种培养料对平菇营养成分的影响[J]. 河南农业科学, 2016, 45(10): 103-106.
[3] 杨阳, 王叶, 范金杰, 等. 转基因棉花 MON757 转化体特异性PCR检测方法及应用[J]. 农业生物技术学报, 2016, 24(6): 908-918.
[4] 徐宝梁. 转基因棉籽培养料栽培平菇过程中Bt蛋白、转入DNA降解及对平菇、平菇害虫的影响[D]. 北京: 中国农业大学, 2005: 65-72.
[5] 于莉. 利用转基因油菜籽栽培平菇安全性分析[D]. 沈阳: 沈阳农业大学, 2007: 39-52.
[6] 马务迢, 王凡华, 谭庆云, 等. 棉籽壳培养基及栽培银耳中转基因成分的PCR分析[J]. 中国食品学报, 2016, 16(3): 168-175.
[7] 闫燕. 转基因培养料与平菇间基因水平转移现象的研究[D]. 保定: 河北农业大学, 2011.
[8] SHANG Ying, YAN Yan, XU Wen-tao, et al. Research on gene mobility and gene flow between genetically modified mon 15985 cotton and pleurotus ostreatus[J]. Journal of Food Safety, 2016, 36(3): 423-432.
[9] 王凡华, 马务迢, 谭庆云, 等. 银耳干品的 DNA 提取及随机扩增多态性分析[J]. 食品科学, 2015, 36(4): 172-175.
[10] 史红鸽, 戚元成, 申进文. 一种从平菇菌丝中提取总RNA的方法[J]. 江西农业学报, 2014, 26(8): 68-70.
[11] CHAI Ran, ZHANG Guang, SUN Qiang, et al. Liposome-mediated mycelial transformation of filamentous fungi[J]. Fungal biology, 2013, 117(9): 577-583.