Abstract
Potato protein was hydrolyzed by trypsin to prepare for ACE inhibitory peptides in our previous researches (the ACE inhibition rates was 61.20%±0.15%), and we used plastein reaction to modify potato protein hydrolysates for enhancing the ACE inhibitory activity of peptides in this study. The influences of the kinds of added amino acids, the substrate/enzyme ratio, pH value, the temperature and hydrolysis time on ACE inhibitory activity of the plastein reaction modification of potato protein hydrolysates were investigated. To achieve the maximum ACE inhibitory activity in the plastein reaction modification of cottonseed protein hydrolysates, the response surface methodology was applied to find the optimum conditions based on the single factor tests. \[E\]/\[S\] ratio at 3 565.05 U/g, substrate concentration at 30.0%, and extrinsic dose of glycine at 0.5 mol/g reacted at 43.68 ℃ and pH 8.05 for 3.0 h, were found to be the optimal conditions, and the ACE inhibitory activity could reached (82.89±0.05)%. Compared with unmodified potato ACE inhibitory peptide, its ACE inhibitory activity increased by 1.35 times.
Publication Date
2-28-2018
First Page
6
Last Page
10,82
DOI
10.13652/j.issn.1003-5788.2018.02.002
Recommended Citation
Dandan, GAO; Zhongren, MA; Reziwanguli, SAIMAITI; Xiaojing, TIAN; Mingsheng, LI; and Shien, CHEN
(2018)
"Plastein reaction to modify the ACE inhibitory peptides of potato protein,"
Food and Machinery: Vol. 34:
Iss.
2, Article 2.
DOI: 10.13652/j.issn.1003-5788.2018.02.002
Available at:
https://www.ifoodmm.cn/journal/vol34/iss2/2
References
[1] 姚玉静, 崔春, 邱礼平, 等. Plastein反应条件及其机理探讨[J]. 中国调味品, 2009, 34(2): 45-48.
[2] DOUCET D, GAUTHIER S F, OTTER D E, et al. Enzyme-induced gelation of extensively hydrolyzed whey proteins by alcalase: comparison with the plastein reaction and characterization of interactions[J]. Journal of Agricultural & Food Chemistry, 2003, 51(20): 6 036-6 042.
[3] 赵新淮, 孙辉. 类蛋白反应在食品蛋白质和活性肽研究中的应用[J]. 东北农业大学学报, 2011, 42(11): 1-8.
[4] 朱晓杰. 海地瓜蛋白酶解物Plastein反应修饰及其ACE抑制活性研究[D]. 青岛: 中国海洋大学, 2012: 14-33.
[5] 吴丹, 李铁晶, 赵新淮. 酪蛋白水解物的酶法修饰优化与抗氧化活性改善[J]. 农业机械学报, 2010, 17(1): 139-144.
[6] 杨锋, 陈锦屏, 阳显莹. 醋蛋水解物的类蛋白反应及对抗氧化活性的影响[J]. 食品工业科技, 2012, 18(1): 152-158.
[7] 张强, 王松华, 蒋胜娟, 等. 双孢蘑菇源抗氧化肽的Plastein反应修饰研究[J]. 科技视界, 2012, 6(17): 39-43.
[8] 王晗欣, 杜双奎, 赵艳, 等. 鹰嘴豆蛋白水解物类蛋白反应修饰与抗氧化活性研究[J]. 中国食品学报, 2015, 15(1): 34-40.
[9] 汪敬科, 赵新准. 三种氨基酸添加下酶法修饰酪蛋白水解物的ACE抑制活性[J]. 食品与发酵工业, 2011, 37(4): 32-26.
[10] 高丹丹, 李海霞, 祁高展. 发酵马铃薯蛋白制备ACE抑制肽[J]. 食品科技, 2015(1): 258-262.
[11] 高丹丹, 孙青青, 郭鹏辉, 等. 发酵马铃薯蛋白制备抗氧化肽[J]. 食品工业科技, 2015, 36(7): 159-162.
[12] 彭剑秋. ACE 抑制肽定量构效关系研究[D]. 上海: 上海理工大学, 2012: 5-35.
[13] 赵元晖, 李八方, 曾明湧. 海地瓜蛋白水解物中ACE抑制肽的分离纯化及合成[J]. 高等学校化学学报, 2012, 33(2): 308-312.
[14] 沈晴晴. Plastein反应修饰海地瓜酶解物制备ACE抑制肽及其机理研究[D]. 青岛: 中国海洋大学, 2014: 11-22.
[15] 许金龙. 酪蛋白水解物Plastein反应修饰产物降血压和抗氧化活性的研究[D]. 哈尔滨: 东北农业大学, 2016: 4-32.
[16] 周丽杰, 韩青, 李智博, 等. 酶法制备耦合 Plastein 反应的牡蛎ACE抑制肽[J]. 食品科学, 2017, 38(6): 104-110.
[17] 高丹丹, 杨璐璐, 祁高展. 响应面法优化蕨麻多糖的提取工艺[J]. 天然产物研究与开发, 2015, 27(6): 1 099-1 104.
[18] 任佳丽, 黄仁贵, 李忠海. 零余子粗多糖超声波辅助提取工艺优化[J]. 食品与机械, 2016, 32(9): 149-153.
[19] 齐娜, 李涵, 张志宇, 等. 新疆红肉苹果多酚的超声波辅助提取工艺优化[J]. 食品与机械, 2016, 32(9): 177-182.