Abstract
In order to purify flavones from Vaccinium Bracteatum Thunb Leaves(VBTL) preliminarily, the static adsorption and desorption properties of four resins were compared. The purification technology of flavones from VBTL was investigated by dynamic adsorption and desorption experiments. The effect of sample concentration, loading sample volume, ethanol solution for desorption, flow rate of the eluent and volume of eluent on adsorption and desorption rate were studied. In addition, the purification effect was evaluated by removal rate of proteins and polysaccharides and HPLC. The results showed that NKA-II resin had a relatively high rate of adsorption and desorption as well as a shorter adsorption time. NKA-II resin was determined as chromatographic column filler. The optimal purification technology was determined as follows: 0.75 mg/mL for sample concentration, 2 bed volume (BV) for loading sample volume, 50% (vv) ethanol solution for desorption, 1 mL/min for the flow rate of the eluent and 3 BV for the volume of eluent. HPLC analysis of flavones from VBTL before and after purifying suggested the purity of purified flavones from VBTL improved significantly, and the removal rate of proteins and polysaccharides reached 76.32% and 65.45% respectively, with the purity of flavones reaching 48.92% .
Publication Date
2-28-2018
First Page
166
Last Page
170,194
DOI
10.13652/j.issn.1003-5788.2018.02.035
Recommended Citation
Dejin, ZHANG; Yiyong, CHEN; Yalin, HU; and Xiang, LIU
(2018)
"Purification technology of flavones from Vaccinium Bracteatum Thunb leaves using macroporous resin,"
Food and Machinery: Vol. 34:
Iss.
2, Article 35.
DOI: 10.13652/j.issn.1003-5788.2018.02.035
Available at:
https://www.ifoodmm.cn/journal/vol34/iss2/35
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