Abstract
The polysaccharide obtained from the branches and leaves of Chrysanthemum indicum was extracted by microwave assisted extraction after its powders decolorized and degreased, and then the polysaccharide extraction content was determined by phenol-sulfuric acid method. The extraction process of the polysaccharide was studied by single factor experiment which controlled liquid-solid ratio, extraction temperature, extraction time, and microwave power, and the orthogonal experiment was tested and based on single factor experiment, then determined the best extraction process. Those free radical cleared effects of its polysaccharide were tested by Fenton reaction and DPPH reaction. The results showed that the polysaccharide extraction content from the branches and leaves of C. indicum could reach 6.32% when solid-liquid ratio was 140 (g/mL), extracted by using microwave power (450 W) at 80 ℃ for 10 min. The anti-oxidant activity tests of the polysaccharide from the branches and leaves of C. indicum showed a good scavenging activity on ·OH and DPPH·, and the relationship between the polysaccharide and hydroxyl free radical clearance was found to be an concentration reliable. Finally, the polysaccharide was verified to be an untapped antioxidant substance.
Publication Date
2-28-2018
First Page
171
Last Page
175
DOI
10.13652/j.issn.1003-5788.2018.02.036
Recommended Citation
Cunfang, LIU; Rui, WU; Hua, ZHAO; and Guanghui, TIAN
(2018)
"Study on microwave-assisted extraction process for polysaccharide from the branches and leaves in Chrysanthemum indicum and its anti-oxidant activity,"
Food and Machinery: Vol. 34:
Iss.
2, Article 36.
DOI: 10.13652/j.issn.1003-5788.2018.02.036
Available at:
https://www.ifoodmm.cn/journal/vol34/iss2/36
References
[1] 王淑荣, 焦锁囤. 火焰原子吸收光谱法测定陕西秦岭不同地域野菊花中的微量元素[J]. 光谱实验室, 2013, 30(1): 325-328.
[2] BUI T T L, BUI H T, NGUYEN P T, et al. Anti-inflammatory components of Chrysanthemum indicum flowers[J]. Bioorganic & Medicinal Chemistry Letters, 2015, 25(2): 266-269.
[3] 郑艺欣, 李奕君, 林埔, 等. 野菊花水提物对果蝇热耐受能力的影响[J]. 食品与机械, 2016, 32 (9): 141-144.
[4] 陶金华, 段金廒, 钱大玮, 等. 菊属药用植物资源化学研究进展[J]. 中国现代中药, 2016, 18(9): 1 212-1 219.
[5] WANG Jun-song, ZHOU Jing, KONG Ling-yi. Three new germacrane-type sesquiterpene stereoisomers from the flowers of Chrysanthemum indicum[J]. Fitoterapia, 2012, 83(8): 1 675-1 679.
[6] LEE B H, NAM T G, PARK W J, et al. Antioxidative and neuroprotective effects of volatile components in essential oils from Chrysanthemum indicum linné flowers[J]. Food Sci. Biotechnol, 2015, 24(2): 717-723.
[7] DU Ning-ning, TIAN Wei, ZHEN Dong-fang, et al. Extraction, purification and elicitor activities of polysaccharides from Chrysanthemum indicum[J]. International Journal of Biological Macromolecules, 2016, 82: 347-354.
[8] KIM S J, LEE K T, CHOI H E, et al. Anti-inflammatory effects of flavonoids in Korean Chrysanthemum species via suppression of inducible nitric oxide synthase and cyclooxygenase-2 in LPS-induced RAW 264.7 Macrophages[J]. Food Sci. Biotechnol, 2015, 24(3): 975-985.
[9] 王桃云, 邱业先, 李斌, 等. 野菊花黄色素提取工艺探索[J]. 食品与机械, 2002, 28(1): 26-27.
[10] 申海进, 郭巧生, 房海灵. 野菊花乙醚提取物的理化性质及抗氧化能力[J]. 食品科学, 2012, 33(15): 43-47.
[11] 国家药典委员会. 中华人民共和国药典: 一部[M]. 2015年版. 北京: 中国医药科技出版社, 2015: 314.
[12] 吴雪松, 许浚, 张铁军, 等. 野菊的化学成分及质量评价研究进展[J]. 中草药, 2015, 46(3): 443-452.
[13] 刘晓丹, 刘存芳, 赖普辉, 等. 野菊花茎叶挥发油的化学成分及其对植物病原真菌抑制作用[J]. 食品工业科技, 2013, 34(24): 98-100.
[14] 陆颖. 碱溶性野菊花多糖的结构分析及免疫活性研究[D]. 南京: 南京中医药大学, 2013: 1-26.
[15] 卫强, 孙晓燕. 菊叶中多糖的含量测定方法研究[J]. 中成药, 2011, 33(3): 532-534.
[16] 宫江宁, 饶玉, 杨义菊, 等. 大孔树脂吸附纯化黔产龙胆草多糖工艺优化[J]. 食品与机械, 2017, 33(5): 178-181.
[17] 田光辉, 刘存芳, 辜天琪, 等. 野生藿香中多糖的提取与测定及抗氧化活性研究[J]. 食品工业科技, 2010, 31(2): 249-251.