Isolation and identification of immunoactive peptides from rice protein hydrolysates with medium-pressure ion exchange chromatography

Authors

CHENG Yunhui, College of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China
MAO Tianmi, College of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China
HUANG Lu, College of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China
CHEN Maolong, College of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China
WEN Li, College of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China
XU Zhou, College of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China

Abstract

Medium-pressure ion exchange chromatography was used for further fractionation of rice immunoactive peptides obtained from Trypsin hydrolyzation and gradient ethanol elution with DA201-C macroporous adsorption resin (40% ethanol fraction). The results showed that the optimal parameters for fractionation of RPHs-A3 fractions by WorkBeads 40 Q on a 2.6 cm×40 cm columns were as follows: Sample: 5 mL, 30 mg/mL 40% ethanol fraction; Equilibration buffer A: pH 9.0, 0.01 mol/L Tris-HCl; Elution Buffer B: pH 9.0, 0.01 mol/L Tris-HCl containing 1 mol/L NaCl; Sample flow rate: 1 mL/min; Elution flow rate: 10 mL/min. Five fractions were obtained after NaCl gradient elution. The results of MTT assay and LPS-stimulated RAW264.7 macrophages inflammation model showed that fraction RPHs-A3-B3 had the highest immune activity. RPHs-A3-B3 produced a significant effect on the proliferation of RAW264.7 macrophages with a stimulation index value of 1.315. Meanwhile, RPHs-A3-B3 significantly decreased the production of NO in LPS-stimulated RAW264.7 macrophages (P＜0.05). The inhibition rate was 8.28%. The research indicated that medium-pressure ion exchange chromatography can effectively fractionate rice immunoactive peptides. The SI value increased from 1.273 to 1.315. And RPHs-A3 had a significant inhibitory effect on intracellular NO release.

Publication Date

3-28-2018

First Page

151

Last Page

155

DOI

10.13652/j.issn.1003-5788.2018.03.032

Recommended Citation

Yunhui, CHENG; Tianmi, MAO; Lu, HUANG; Maolong, CHEN; Li, WEN; and Zhou, XU (2018) "Isolation and identification of immunoactive peptides from rice protein hydrolysates with medium-pressure ion exchange chromatography," Food and Machinery: Vol. 34: Iss. 3, Article 32.
DOI: 10.13652/j.issn.1003-5788.2018.03.032
Available at: https://www.ifoodmm.cn/journal/vol34/iss3/32

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