Optimization of extraction process for total triterpenoids in Alisma orientalis (Sam.) Juzep. by response surface methodology

Authors

SHI Feng, Agronomy College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Crop Physiology, Ecology and Cultivation in Southwest, Ministry of Agriculture, Chengdu, Sichuan 611130, China
CHEN Yu, Agronomy College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Crop Physiology, Ecology and Cultivation in Southwest, Ministry of Agriculture, Chengdu, Sichuan 611130, China
ZHANG Yaqin, Agronomy College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Crop Physiology, Ecology and Cultivation in Southwest, Ministry of Agriculture, Chengdu, Sichuan 611130, China
MA Liuhui, Agronomy College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Crop Physiology, Ecology and Cultivation in Southwest, Ministry of Agriculture, Chengdu, Sichuan 611130, China
DOU Mingming, Agronomy College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Crop Physiology, Ecology and Cultivation in Southwest, Ministry of Agriculture, Chengdu, Sichuan 611130, China
YANG Zhengming, Agronomy College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Crop Physiology, Ecology and Cultivation in Southwest, Ministry of Agriculture, Chengdu, Sichuan 611130, China
CHEN Xingfu, Agronomy College of Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Crop Physiology, Ecology and Cultivation in Southwest, Ministry of Agriculture, Chengdu, Sichuan 611130, China

Abstract

The optimum conditions of ultrasonic extraction of total triterpenoid from leaves of Alisma orientalis (Sam.) Juzep. were investigated in this study. On the basis of single factor experiments, with yields of total triterpenoid as indexes, the three levels of four factors of Box-Behnken experimental design and response surface methodology was adopted to optimize extraction conditions by taking extraction temperature ,ethanol concentration, extraction time and the ratio of material to solvent as factors. Results: Optimal extraction technology was as following, i.e. the ethanol concentration of 70%, the extracting for 46.00 min at 57.00 ℃, and the ratio of material to solvent was 114 (g/mL), ultrasonic time for 30 min. Then the total triterpenes extraction rate reached 2.98% in this condition.

Publication Date

3-28-2018

First Page

170

Last Page

174

DOI

10.13652/j.issn.1003-5788.2018.03.036

Recommended Citation

Feng, SHI; Yu, CHEN; Yaqin, ZHANG; Liuhui, MA; Mingming, DOU; Zhengming, YANG; and Xingfu, CHEN (2018) "Optimization of extraction process for total triterpenoids in Alisma orientalis (Sam.) Juzep. by response surface methodology," Food and Machinery: Vol. 34: Iss. 3, Article 36.
DOI: 10.13652/j.issn.1003-5788.2018.03.036
Available at: https://www.ifoodmm.cn/journal/vol34/iss3/36

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