Abstract
In order to investigate the inhibition of lipase by theabrownin and polysaccharide of black tea, the theabrownin and polysaccharides were extracted from the same black tea in different regions. The inhibition rate of lipase activity of theabrownin and polysaccharide was analyzed by p-nitrophenol method, and the inhibitory mechanism of theabrownin and polysaccharide on lipase was discussed. The results showed that the inhibition of lipase by theabrownin and polysaccharide of black tea was significant. The IC50 of Pu-er theabrownin and polysaccharide were 25.96 and 47.57 mg/mL, respectively, which were the minimum, and the inhibitory effect was the best, secondly for liupu tea. The worst inhibitory effect was that the theabrownin and polysaccharide of fu-brick. The inhibition effects of theabrownin and polysaccharide of Kang brick were between those of the liupu tea and fu-brick. The inhibition types of theabrownin of each black tea were the complex of reversible competitive and noncompetitive, while the liupu tea was closed to the competitive inhibition type. The inhibition types of polysaccharide of Pu-er tea, liupu tea and Kang brick were the complex of reversible competitive and noncompetitive, while the polysaccharide of fu-brick was reversible competitive inhibition.
Publication Date
3-28-2018
First Page
27
Last Page
31,58
DOI
10.13652/j.issn.1003-5788.2018.03.006
Recommended Citation
Xianglong, LI; Xiaomei, LI; Xu, YANG; Lei, TANG; and Jingyi, XU
(2018)
"Study of inhibition of black tea theabrownin and tea polysaccharides on lipase,"
Food and Machinery: Vol. 34:
Iss.
3, Article 6.
DOI: 10.13652/j.issn.1003-5788.2018.03.006
Available at:
https://www.ifoodmm.cn/journal/vol34/iss3/6
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