Abstract
Static adsorption and desorption experiments were carried out to determine the types of macroporous resins for purifying flavonoids from eggplant leaves, to optimize its technological conditions, and to comparatively analyze the antioxidant activities of eggplant leave flavonoids before and after purification. The results showed that NKA-II was the optimal adsorbent. The optimum conditions for purification of flavonoids from eggplant leaves were listed as follows: sample concentration of crude extracts 0.362 6 mg/mL, sample pH 2, the static absorption time, 2.5 h. After 40 minutes desorption with 70% ethanol (pH=3), the content of flavonoids was increased from 17.91% before purification to 64.59%, the rate of purification was improved by 3.61 times. Compared with rutin, scavenging activity of flavonoids after purification on ABTS+· and DPPH· was less, and scavenging activity of flavonoids after purification on ABTS+· was stronger than before purification.
Publication Date
5-28-2018
First Page
158
Last Page
163
DOI
10.13652/j.issn.1003-5788.2018.05.033
Recommended Citation
Bin, YANG; Xiaoqing, JIN; Caixia, LI; and Oyang, JIA
(2018)
"Purification of flavonoids from eggplant leaves using macroporous resin and its antioxidant activities,"
Food and Machinery: Vol. 34:
Iss.
5, Article 33.
DOI: 10.13652/j.issn.1003-5788.2018.05.033
Available at:
https://www.ifoodmm.cn/journal/vol34/iss5/33
References
[1] XIE Chuan-qi, WANG Hai-long, SHAO Yong-ni, et al. Different algorithms for detection of malondialdehyde content in eggplant leaves stressed by grey mold based on hyperspectral imaging technique[J]. Intelligent Automation & Soft Computing, 2015, 21(3): 395-407.
[2] 莫天录, 薛林贵, 高慧, 等. 大孔吸附树脂纯化绿茄叶黄酮的工艺研究[J]. 天然产物研究与开发, 2015, 27(3): 534-539
[3] 刘一杰, 薛永常. 植物黄酮类化合物的研究进展[J]. 中国生物工程杂志, 2016, 36(9): 81-86.
[4] CZAPLINSKA M, CZEPAS J, GWOZDZINSKI K. Structure antioxidative and anticancer properties of flavonoids[J]. Postepy Biochem, 2012, 58 (3): 235-244.
[5] 卫静莉, 高松平, 董 梅, 等. 柿叶提取黄酮类化合物方法及鉴定[J]. 林业科技开发, 2007, 21(3): 47-49.
[6] 刘 露, 龙媛媛, 郭宗雨, 等. 欧亚旋覆花总黄酮对糖基化终末产物诱导L929细胞衰老及RAGE表达的影响[J]. 中草药, 2018, 49(3): 626-632.
[7] 何伟平, 王勤志, 朱晓韵, 等. 茄子提取物对清除自由基作用的研究[J]. 轻工科技, 2012(4): 3-4.
[8] LIU Chang-qing, JIAO Rui-hua, YAO Ling-yuan, et al. Adsorption characteristics and preparative separation of chaetominine from Aspergillus fumigatus mycelia by macroporous resin[J]. Journal of Chromatography B, 2016, 1 015: 135-141.
[9] 李琼, 陈恺, 陈燕勤, 等. 大孔吸附树脂分离纯化核桃青皮总多酚[J]. 食品与机械, 2015, 31(1): 175-180.
[10] DONG Yi, ZHAO Mou-ming, SUN-WSTERHOUSE Dong-xiao, et al. Absorption and desorption behaviour of the flavonoids from Glycyrrhiza glabra L. leaf on macroporous adsorption resins[J]. Food Chemistry, 2015, 168: 538-545.
[11] 高海宁, 李彩霞, 张勇, 等. “黑美人”土豆色素体外抗氧化性研究[J]. 天然产物研究与开发, 2012, 24(2): 224-228, 233.
[12] WU Ya-jun, LIU Jing-jing, GU Su, et al. Orthogonal strategy development using reversed macroporous resin coupled with hydrophilic interaction liquid chromatography for the separation of ginsenosides from ginseng root extract[J]. Journal of Separation Science, 2017, 40(21): 4 128-4 134.
[13] WU Yuan-feng, ZHANG Lei, MAO Jian-wei, et al. Kinetic and thermodynamic studies of sulforaphane adsorption on macroporous resin[J]. Journal of Chromatography B, 2016, 1 028(15): 231-236.
[14] TANG Liu, WEI Xing-chen, AN Fa-liang, et al. Preparative separation of TL1-1 from Daldinia eschscholzii extract by macroporous resin and evaluation of its antimicrobial activities[J]. Journal of Chromatography B, 2017, 1 060(15): 22-29.
[15] LI Lu, LIU Ju-zhao, LUO Meng, et al. Efficient extraction and preparative separation of four main isoflavonoids from Dalbergia odorifera T. Chen leaves by deep eutectic solvents-based negative pressure cavitation extraction followed by macroporous resin column chromatography[J]. Journal of Chromatography B, 2016, 1 033: 40-48.
[16] 吕英华, 苏平, 那宇, 等. 桑葚色素体外抗氧化能力研究[J]. 浙江大学学报: 农业与生命科学版, 2007, 3(1): 102-107.
[18] 张冰洁. 金银花叶中黄酮类化合物的提取、纯化及其抗氧化研究[D]. 洛阳： 河南科技大学, 2017.