Optimization on the enzymatic preparation of extracellular polysaccharide from Porphyridium creuntum with antioxidant activities in vitro

Authors

DONG Le, College of Ocean and Food Science, Quanzhou Normal University, Quanzhou, Fujian 362000, China; Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou, Fujian 362000, China; Key Laboratory of Inshore Resources
HE Jingyan, College of Ocean and Food Science, Quanzhou Normal University, Quanzhou, Fujian 362000, China; Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou, Fujian 362000, China; Key Laboratory of Inshore Resources
ZHENG Tingting, College of Ocean and Food Science, Quanzhou Normal University, Quanzhou, Fujian 362000, China; Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou, Fujian 362000, China; Key Laboratory of Inshore Resources
DAI Congjie, College of Ocean and Food Science, Quanzhou Normal University, Quanzhou, Fujian 362000, China; Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou, Fujian 362000, China; Key Laboratory of Inshore Resources
HUANG Weihong, College of Ocean and Food Science, Quanzhou Normal University, Quanzhou, Fujian 362000, China; Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou, Fujian 362000, China; Key Laboratory of Inshore Resources
LI Yuanyue, Key Laboratory of Marine Fisheries Resources and Environment of Fujian Province, Jimei, Fujian 361000, China

Abstract

The optimum enzymatic conditions of extracellular polysaccharide of Porphyridium creuntum (EPS) were investigated in this research. Based on the results of single factor experiment, by using the Box-Behnken response surface method array design, the optimum conditions for hydrolyzing EPS to obtain antioxidant hydrolysates with the highest hydroxyl radical scavenging activity were as follows: enzymolysis reaction time 6.3 h, enzymolysis reaction pH 8.0, enzymolysis temperature 63 ℃, and enzyme-to-substrate ratio 2.7 U/mg. Under the conditions, the scavenging rate of hydroxyl radical was 70.64%. Subsequently, the antioxidant activity of the enzymatic hydrolysates were determined in vitro. The results showed that the enzymatic hydrolysates had good scavenging abilities on ·OH, DPPH·, ABTS⁺· and O^-₂·, the half-scavenging concentration values of the crude hydrolysates against ·OH, DPPH·, ABTS⁺· and O^-₂· radicals were (1.67±0.58)mg/mL, (1.08±0.01) mg/mL, (0.64±0.01) mg/mL and (4.16±0.01) mg/mL respectively.

Publication Date

5-28-2018

First Page

177

Last Page

182

DOI

10.13652/j.issn.1003-5788.2018.05.037

Recommended Citation

Le, DONG; Jingyan, HE; Tingting, ZHENG; Congjie, DAI; Weihong, HUANG; and Yuanyue, LI (2018) "Optimization on the enzymatic preparation of extracellular polysaccharide from Porphyridium creuntum with antioxidant activities in vitro," Food and Machinery: Vol. 34: Iss. 5, Article 37.
DOI: 10.13652/j.issn.1003-5788.2018.05.037
Available at: https://www.ifoodmm.cn/journal/vol34/iss5/37

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