Isolation,identification,multilocus sequence typing analysis and antimicrobial resistance of Campylobacter jejuni isolated from commercial chicken in northeast China

Authors

FEI Peng, College of Food and Biological Engineering, Henan University of Science and Technology, Luoyang, Henan 471023, China
ZHAO Shengjuan, College of Food and Biological Engineering, Henan University of Science and Technology, Luoyang, Henan 471023, China
JIANG Yichao, Changbai Mountain Food and Drug Inspection Testing Center, Baishan, Jilin 134500, China
JIANG Yan, MuLeng City Food Inspection Testing Center, Mudanjiang, Heilongjiang 157500, China
LIU Lili, College of Food and Biological Engineering, Henan University of Science and Technology, Luoyang, Henan 471023, China
XIAO Feng, College of Food and Biological Engineering, Henan University of Science and Technology, Luoyang, Henan 471023, China
ZHANG Huiyun, College of Food and Biological Engineering, Henan University of Science and Technology, Luoyang, Henan 471023, China
TANG Haoguo, College of Food and Biological Engineering, Henan University of Science and Technology, Luoyang, Henan 471023, China
KANG Huaibin, College of Food and Biological Engineering, Henan University of Science and Technology, Luoyang, Henan 471023, China

Abstract

In order to reveal the contamination, population characteristics and antimicrobial resistance of Campylobacter jejuni isolated from commercial chicken in Northeast China, 1 000 chicken samples were collected from the Northeast China. C. jejuni was isolated and identified.The genetic diversity of C. jejuni was analyzed by multilocus sequence typing (MLST). The antimicrobial resistance of C. jejuni to 8 antibiotics was detected by K-B AGAR diffusion method. The results indicated that 62 C. jejuni strains were isolated from 1 000 chicken samples with a contamination rate of 6.2%, and were divided into 14 sequence types (STs). Among them, ST1 and ST5 were the dominant STs. The results of antimicrobial resistance analysis showed that 62 C. jejuni strains were highly sensitive to ciprofloxacin, compound sinomin and cefotaxime.

Publication Date

6-28-2018

First Page

46

Last Page

49

DOI

10.13652/j.issn.1003-5788.2018.06.010

Recommended Citation

Peng, FEI; Shengjuan, ZHAO; Yichao, JIANG; Yan, JIANG; Lili, LIU; Feng, XIAO; Huiyun, ZHANG; Haoguo, TANG; and Huaibin, KANG (2018) "Isolation,identification,multilocus sequence typing analysis and antimicrobial resistance of Campylobacter jejuni isolated from commercial chicken in northeast China," Food and Machinery: Vol. 34: Iss. 6, Article 10.
DOI: 10.13652/j.issn.1003-5788.2018.06.010
Available at: https://www.ifoodmm.cn/journal/vol34/iss6/10

References

[1] 吴清平, 钟显, 张菊梅. 空肠弯曲杆菌生物膜形成和调控机理研究进展[J]. 微生物学报, 2016, 56(2): 180-187.
[2] COLLADO L, MUOZ N, PORTE L, et al. Genetic diversity andclonal characteristics of ciprofloxacin-resistant Campylobac-ter jejuni isolated from Chilean patients with gastroenteritis[J]. Infection Genetics & Evolution, 2018, 58: 290-293.
[3] MOHAN V, STEVENSON M A, MARSHALL J M, et al.Characterisation by multilocus sequence and porA and flaA typing of Campylobacter jejuni isolated from samples of dog faeces collected in one city in New Zealand[J]. N Z Vet J, 2017, 65(4): 1-12.
[4] 赵鑫, 张凤丽. 应用HACCP有效防止鸡肉加工熟食过程中的空肠弯曲杆菌感染[J]. 中国畜牧兽医文摘, 2015(6): 201-209.
[5] 钟显. 食品中空肠弯曲杆菌风险识别及菌株特性研究[D]. 广州: 广东工业大学, 2016: 1.
[6] MA Hui, SU Yu-lan, MA Lu-yao, et al. Prevalence and characterization of Campylobacter jejuni isolated from retail chicken in Tianjin, China[J]. Journal of Food Protection, 2017, 80(6): 1 032-1 040.
[7] GARCASNCHEZ L, MELERO B, JAIME I, et al. Campylobacter jejuni survival in a poultry processing plant environment[J]. Food Microbiology, 2017, 65: 185-192.
[8] CODY A J, BRAY J E, JOLLEY K A, et al. A core genome multi-locus sequence typing scheme for stable, comparative analyses of Campylobacter jejuni and C. coli human disease isolates[J]. Journal of Clinical Microbiology, 2017, 55(7): 2 086-2 097.
[9] YANG Wan-na, ZHANG Mao-jun, ZHOU Ji-yuan, et al. The molecular mechanisms of ciprofloxacin resistance in clinical Campylobacter jejuni and their genotyping characteristics in Beijing, China[J]. Foodborne Pathogens & Disease, 2017, 14(7): 386-392.
[10] VINUEZABURGOS C, WAUTIER M, MARTINY D, et al.Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age[J]. Poultry Science, 2017, 96(7): 2 366-2 374.
[11] 陈荀, 刘书亮, 吴聪明, 等. 鸡肉生产链中空肠弯曲杆菌的污染分析及PFGE分型研究[J]. 中国人兽共患病学报, 2012, 28(10): 1 012-1 016.
[12] 费鹏, 杨同香, 姜亦超, 等. 克罗诺杆菌分型技术研究进展[J]. 食品科学, 2017, 38(21): 308-312.
[13] 洪捷, 马恺, 顾玲. 江苏地区空肠弯曲菌多位点序列分型和毒力基因分析[J]. 中国卫生检验杂志, 2017(7): 1 006-1 010.
[14] 黄金林, 许海燕, 张弓, 等. 江苏奶牛空肠弯曲菌和结肠弯曲菌流行状况及耐药性分析[J]. 中国人兽共患病学报, 2007, 23(10): 1 016-1 020.
[15] 周倩, 唐梦君, 张小燕, 等. 江苏省禽源空肠弯曲杆菌的分离鉴定及耐药性研究[J]. 中国人兽共患病学报, 2017, 33(6): 495-500.
[16] LVESQUE S, FROST E, ARBEIT R D, et al. Multilocus sequence typing of Campylobacter jejuni isolates from humans, chickens, raw Milk, and environmental water in Quebec, Canada[J]. Journal of Clinical Microbiology, 2008, 46(10): 3 404-3 411.
[17] 韩新锋, 刘书亮, 张晓利, 等. 鸡肉空肠弯曲杆菌的分离鉴定及耐药性分析[J]. 中国人兽共患病学报, 2012, 28(1): 31-36.
[18] 陈栎娜, 陆思琴, 陈荀, 等. 鸡肉空肠弯曲杆菌的污染率与耐药性分析[J]. 中国兽医杂志, 2013, 49(2): 7-9.
[19] 董俊, 韩梅, 周康, 等. 47株空肠弯曲菌湖北禽源株的多位点序列分型[J]. 微生物学报, 2016, 56(1): 150-156.