Abstract
Extracted lignan from flaxseed meal by reflux method. The crude extract was preliminary purified by D101 macroporous adsorption resins which was followed by semi-preparation HPLC separation. The semi-preparative HPLC conditions were as follows: column was Megres C18(30 mm×250 mm，10 μm), mobile phase was a mixture of acetonitrile(A)-0.1% phosphoric acid water solution(B), gradient elution,flow rate was 35 mL/min, wavelength was 280 nm, column temperature was 30 ℃ and injection volume was 2 mL. In the conditions, 98% purity flax lignans were obtained. Investigated the protecting activity of lignan against DNA damage that caused by UV photolysis and H2O2 treatment, it reveals that flax lignans has a significant effect on the protection of DNA oxidative damage while the concentration is 0.07~0.40 μg/μL.
Publication Date
6-28-2018
First Page
139
Last Page
144
DOI
10.13652/j.issn.1003-5788.2018.06.029
Recommended Citation
Yongchao, ZHANG; Li, ZHANG; Yunsong, ZHANG; Yuanyuan, JIANG; and Yichao, WU
(2018)
"The purification of lignin from linseed meal and its protective effect on oxidative plasmid DNA damage,"
Food and Machinery: Vol. 34:
Iss.
6, Article 29.
DOI: 10.13652/j.issn.1003-5788.2018.06.029
Available at:
https://www.ifoodmm.cn/journal/vol34/iss6/29
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