Study on the extraction technology, physicochemical properties and bioactivity of the polysaccharide form Radix Glehniae

Authors

JING Yongshuai, College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China
SU Lei, College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China
HAN Yu, College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China
ZHANG Danshen, College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China
ZHANG Ruijuan, College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China
WU Lanfang, College of Pharmacology, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China
ZHENG Yuguang, College of Pharmacology, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China
QIN Xuan, Department of International Rehabilitation, The Second Hospital of Shijiazhuang, Shijiazhuang, Hebei 050062, China

Abstract

Response surface methodology based on single-factor experiment was applied to optimize the extraction conditions of polysaccharide. As a result, the optimum extraction conditions were extraction temperature 65 ℃, ultrasonic time 22 min, liquid to material ratio of 221 （mL/g）. Under the control of such conditions, the extraction rate of polysaccharide from Radix Glehniae was 12.13%. The polysaccharide from Radix Glehniae had typical characteristics of polysaccharides, containing 90.4% of the total sugar and 1.22% protein, and the thermal degradation temperature was 300.32 ℃. The scavenging abilities of DPPH free radicals (IC₅₀=1.99 mg/mL), OH free radicals (IC₅₀=1.71 mg/mL) and the inhibiting ability of α-glucosidase (IC₅₀ =5.23 mg/mL) were investigated. The results showed that polysaccharide from Radix Glehniae had certain free radicals scavenging and potential hypoglycemic activities.

Publication Date

6-28-2018

First Page

152

Last Page

157

DOI

10.13652/j.issn.1003-5788.2018.06.031

Recommended Citation

Yongshuai, JING; Lei, SU; Yu, HAN; Danshen, ZHANG; Ruijuan, ZHANG; Lanfang, WU; Yuguang, ZHENG; and Xuan, QIN (2018) "Study on the extraction technology, physicochemical properties and bioactivity of the polysaccharide form Radix Glehniae," Food and Machinery: Vol. 34: Iss. 6, Article 31.
DOI: 10.13652/j.issn.1003-5788.2018.06.031
Available at: https://www.ifoodmm.cn/journal/vol34/iss6/31

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