Abstract
To improve the yield and quality of Osmanthus extracts, several techniques were applied including the enzymatic hydrolysis by β-glucosidase and pectinase complex along with petroleum ether extraction and GC-MS analysis. The enzymatic hydrolysis process was optimized usingresponse surface methodology, and a reliable multiple quadratic regression model was established. The results showed that the best levels of the factors were as followed: pH 4.7, time 2.6 h, temperature 46 ℃, ratio of material to liquid 19.81 (mL/g), and the amount of enzyme complex 54.4 IU/g with 48.1% β-glucosidase activity, which was confirmed by experiments with an extraction yield of 3.32%, an increment of 62.75% compared to direct extraction. The results of GC-MS indicate an increase of main compounds, revealing that the hydrolysis by enzyme complex is more effective than direct extraction in terms of the extraction quality improvement. Compared with the control, the contents of the main aroma substances ,such as dihydrolinalool, γ-decalactone, dihydro-β-ionone, β-ionone, geraniol, and limonene, were increased by 27.27%, 116.36%, 100.00%, 247.06%, 72.84% and 14.29%, respectively. Meanwhile, nerolidol and β-ionol were also detected by GC-MS. After treating by β-glucosidase-pectinase enzyme, the yield and aroma quality of Osmanthus abstracts improved effectively.
Publication Date
6-28-2018
First Page
180
Last Page
186
DOI
10.13652/j.issn.1003-5788.2018.06.036
Recommended Citation
Xuesong, ZHANG; Jianjun, PEI; Linguo, ZHAO; Feng, TANG; and Xianying, FANG
(2018)
"Optimization on preparation of Osmanthus extracts by enzymes treatment,"
Food and Machinery: Vol. 34:
Iss.
6, Article 36.
DOI: 10.13652/j.issn.1003-5788.2018.06.036
Available at:
https://www.ifoodmm.cn/journal/vol34/iss6/36
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