Quick determination of chemical constituents in Prunus cerasifera Leave by UPLC-UV-QDa

Authors

LIU Wei, University and College Key Lab of Natural Product Chemistry and Application in Xinjiang, Yili Normal University, Yining, Xinjiang 835000, China
LIU Tianqi, University and College Key Lab of Natural Product Chemistry and Application in Xinjiang, Yili Normal University, Yining, Xinjiang 835000, China
GE Yuwei, University and College Key Lab of Natural Product Chemistry and Application in Xinjiang, Yili Normal University, Yining, Xinjiang 835000, China
OUYANG Yan, University and College Key Lab of Natural Product Chemistry and Application in Xinjiang, Yili Normal University, Yining, Xinjiang 835000, China

Abstract

UPLC-UV-QDa technique, combined with the acidic hydrolysis experiment were applied to quantitative and qualitative analysis flavonoid aglycones and methanol extracts from leaves of four different kinds of Prunus cerasifera. The result showed that quercetin and kaempferol were well separated in 7.0 minutes and had good linear relationship, the average recoveries of two flavonoid aglycones were 98.65% and 102.02% with RSD 1.08%(n=3) and 1.17%(n=3), the method was proved to be fast, accurate and repeatable. There was some difference in the content of flavonoid aglycone from different kinds of Prunus cerasifera in different environment, two flavonoid aglycones were found in all kinds of leaves, while kaempferol was the main composition. The content of kaempferol was the highest in leaves of purple fruit in sunny slope up to (7.68±0.45) mg/g, and the lowest in leaves of red fruit in valleys with (4.59±0.32) mg/g, the content of total flavonoids were from 16.21 to 33.25 mg/g. Composition of extracts from leaves of purple fruit in sunny slope were rapidly identified by UPLC-UV-QDa technique, including one caffeoylquinic acid, four quercetin glycosides and seven kaempferol glycosides. The above results provide theoretical support and technical basis for the further exploitation and utilization of Prunus cerasifera.

Publication Date

9-28-2018

First Page

96

Last Page

99,134

DOI

10.13652/j.issn.1003-5788.2018.09.020

Recommended Citation

Wei, LIU; Tianqi, LIU; Yuwei, GE; and Yan, OUYANG (2018) "Quick determination of chemical constituents in Prunus cerasifera Leave by UPLC-UV-QDa," Food and Machinery: Vol. 34: Iss. 9, Article 20.
DOI: 10.13652/j.issn.1003-5788.2018.09.020
Available at: https://www.ifoodmm.cn/journal/vol34/iss9/20

References

[1] 中国植物志编委会. 中国植物志: 第三十八卷[M]. 北京: 科学出版社, 1986: 38.
[2] FAUST M, SURNYI D. Origin and dissemination of plums[J]. Horticultural Reviews, 1999, 23: 179-231.
[3] 刘影, 张相锋, 赵玉, 等. 新疆濒危野生樱桃李的种群结构与动态[J]. 生态学杂志, 2013, 32(7): 1 762-1 769.
[4] 王春梅, 苏普, 谢天成, 等. 野生樱桃李营养成分及抗氧化功能研究进展[J]. 药物生物技术, 2013, 20(6): 582-585.
[5] 姜洪芳, 张卫明, 张玖. 樱桃李色素提取及稳定性研究[J]. 中国野生植物资源, 2008, 27(5): 56-59.
[6] 李紫薇, 欧阳艳, 贾风勤, 等. 野酸梅皮色素抗氧化活性研究[J]. 食品科技, 2011, 36(6): 269-271.
[7] WANG Yan, CHEN Xiao-liu, ZHANG Yan-min, et al. Antioxidant activities and major anthocyanins of myrobalan plum (Prunus cerasifera Ehrh.)[J]. Journal of Food Science, 2012, 77(4): C388-C393.
[8] 刘伟, 何晓燕, 陈文强, 等. 响应面优化野生樱桃李叶总黄酮的超声辅助提取工艺[J]. 天然产物研究与开发, 2015, 27(10): 1 765-1 770.
[9] 刘伟, 李紫薇, 陈文强, 等. 新疆野生樱桃李枝、叶不同萃取部位抗氧化活性研究[J]. 食品工业科技, 2016, 37(3): 119-122.
[10] 刘伟, 腊萍, 杨如箴, 等. 野生樱桃李清除DPPH自由基能力及抑制α-葡萄糖苷酶活性[J]. 江苏农业科学, 2017, 45(17): 183-185.
[11] 吴文杰, 周伟娥, 张元, 等. LC-MS/MS技术在中药化学成分分析中的应用[J]. 时珍国医国药, 2016, 27(11): 2 735-2 737.
[12] 牛广财, 闫公昕, 朱丹, 等. Folin-Ciocalteu比色法测定沙棘酒中总多酚含量的工艺优化[J]. 食品与机械, 2016, 32(4): 80-83, 142.
[13] 韩馥蔓, 王莉鑫, 陈影, 等. HPLC同时测定山豆根中7种生物碱及3种黄酮的含量[J]. 中国中药杂志, 2016, 41(24): 4 628-4 634.
[14] STICHER O. Quality of Ginkgo preparations[J]. Planta Medica, 1993, 59(1): 2-11.
[15] 诸姮, 胡宏友, 卢昌义, 等. 植物体内的黄酮类化合物代谢及其调控研究进展[J]. 厦门大学学报: 自然科学版, 2007, 46(1): 136-143.
[16] KAUSE A, OSSIPOV V, HAUKIOJA E, et al. Multiplicity of biochemical factors determining quality of growing birch leaves[J]. Oecologia, 1999, 120(1): 102-112.
[17] LIU Wei, YU Yan-ying, YANG Ru-zhen, et al. Optimization of total flavonoid compound extraction from Gynura medica leaf using response surface methodology and chemical composition analysis[J]. International Journal of Molecular Sciences, 2010, 11(11): 4 750-4 763.
[18] LI Chun-yang, FENG Jin, HUANG Wu-yang, et al. Composition of polyphenols and antioxidant activity of rabbiteye blueberry (Vaccinium ashei) in Nanjing[J]. Journal of Agricultural & Food Chemistry, 2013, 61(3): 523-531.
[19] 杨宝, 范真, 朱锦萍, 等. 金鸡脚化学成分研究[J]. 中草药, 2014, 45(21): 3 053-3 056.
[20] 连冠, 初正云, 王添敏, 等. 刺槐花的化学成分研究（Ⅱ）[J]. 中草药, 2011, 42(9): 707-709.
[21] 赵春超, 邵建华, 张玉伟, 等. 凤眼草化学成分的分离与鉴定[J]. 沈阳药科大学学报, 2009, 26(10): 800-802.
[22] 田平平, 李仁宙, 简永健, 等. 核桃青皮的强抗氧化活性成分及其抗氧化稳定性[J]. 中国农业科学, 2016, 49(3): 543-553.
[23] 柳建军, 刘锡葵. 黄连木食用部位化学成分研究[J]. 中草药, 2009, 40(2): 186-189.
[24] 梁英, 朱志仁, 潘英明, 等. 罗汉果叶中山奈酚-3,7-O-α-L-二鼠李糖苷的提取及自由基清除活性[J]. 食品与发酵工业, 2010(10): 196-198.
[25] 巴寅颖, 刘倩颖, 石任兵, 等. 鬼箭羽中黄酮类化学成分研究[J]. 中草药, 2012, 43(2): 242-246.
[26] 李颖畅, 王玉华, 韩美洲, 等. 蓝莓叶多酚组成成分分析[J]. 食品科学, 2016, 37(6): 106-110.