Abstract
In order to investigate the potential migration of antibiotic resistant (ART) bacteria from cold fresh chicken to humans during handling in production and consumption, selective culture technology and IonS5TM XL sequencing platform were used for counting and diversity analysis of tetracycline-(TETr) and sulfamethoxazole-resistant bacteria (SULr) on the surface of six handlers’ hands contacting with cold fresh chicken products. The results showed that the two ART bacteria from the handlers’ hands significantly increased to 4.80×10～2.66×106 CFU/cm2 after contacting with cold fresh chicken. Furthermore, 21 and 19 genera of shared flora were identified in the TETr and SULr bacteria, respectively, among which the multi-drug resistance of avian and human Acinetobacter and other eight genera bacteria had been already confirmed by extensive research, and the potential transmission of antibiotic resistance genes between avian and human isolates were existed. However, the characteristics of antibiotic resistance of Buttiauxella and other 14 genera had not been reported yet. Our results illustrated the migration of TETr and SULr bacteria from cold fresh chicken products to the handlers' hands by direct contact, and provided a basis for risk assessment of antibiotic resistance exposure to chicken products handlers.
Publication Date
10-28-2019
First Page
52
Last Page
57
DOI
10.13652/j.issn.1003-5788.2019.10.010
Recommended Citation
Liujuan, HUANG; Yi, SHAO; Bo, FENG; and Changyan, ZHOU
(2019)
"Diversity of antibiotic resistant bacteria isolated from cold fresh chicken handlers’ hands,"
Food and Machinery: Vol. 35:
Iss.
10, Article 10.
DOI: 10.13652/j.issn.1003-5788.2019.10.010
Available at:
https://www.ifoodmm.cn/journal/vol35/iss10/10
References
[1] CENTERZ T J. Efforts to slacken antibiotic resistance: Labeling meat products from animals raised without antibiotics in the United States[J]. Science of the Total Environment, 2016, 563(9): 1 088-1 094.
[2] GUAN Xiang-dong, HE Li-xian, HU Bi-jie, et al. Laboratory diagnosis, clinical management and infection control of the infections caused by extensively drug-resistant Gram-negative bacilli: A Chinese consensus statement[J]. Clinical Microbiology and Infection, 2016, 22(S1): S15-S25.
[3] Centers for Disease Control and Prevention. Handwashing: cleanhands save lives. (2019-06-13)[2019-07-18]. http://www.cdc.gov/handwashing/index.html.
[4] 邵小平, 茅艇华, 蒋卓娟, 等. 手卫生管理系统对降低ICU多药耐药菌感染的影响[J]. 中华医院感染学杂志, 2017, 27(17): 4 053-4 055.
[5] WANG Hua, MANUZON M, LEHMAN M, et al. Food commensal microbes as a potentially important avenue in transmitting antibiotic resistance genes[J]. FEMS Microbiology Letters, 2006, 254(2): 226-231.
[6] 张利锋. 山东商品鸡养殖、屠宰、销售环节中细菌耐药性传播规律研究[D]. 北京: 中国疾病预防控制中心, 2016: 13-15.
[7] TAN S, LEE Y, MAHYUDIN A. Antimicrobial resistance of Escherichia coli, and Staphylococcus aureus, isolated from food handler's hands[J]. Food Control, 2014, 44(3): 203-207.
[8] 张可欣, 李忠海, 任佳丽. 食源性细菌耐药性检测方法的研究进展[J]. 食品与机械, 2018, 34(2): 181-184, 212.
[9] QIAO Min, YING Guang-guo, SINGER S, et al. Review of antibiotic resistance in China and its environment[J]. Environment International, 2018, 110: 160-172.
[10] WANG Hua. Commensal bacteria, microbial ecosystems, and horizontal gene transmission: Adjusting our focus for strategic breakthroughs against antibiotic resistance[M]. JAYKUS L, WANG H, SCHLESINGER L. Foodborn Microbes: Shaping the Host Ecosystems. Washington DC: ASM Press, 2009: 267-281.
[11] ROSSI F, RIZZOTTI L, FELIS E, et al. Horizontal gene transfer among microorganisms in food: Current knowledge and future perspective[J]. Food Microbiology, 2014, 42(9): 232-243.
[12] ZHANG Peng, SHEN Zhang-qi, ZHANG Chun-ping, et al. Surveillance of antimicrobial resistance among Escherichia coli, from chicken and swine, China, 2008-2015[J]. Veterinary Microbiology, 2017, 203: 49-55.
[13] 李姝, 邵毅, 周昌艳, 等. 市售鸡肉及内脏中磺胺耐药菌污染特征[J]. 食品科学, 2017, 38(21): 170-174.
[14] 邵毅, 李姝, 黄柳娟, 等. 市售鸡肉中四环素耐药菌污染特征初探[J]. 中国食品学报, 2018, 18(1): 250-256.
[15] 费鹏, 赵胜娟, 姜亦超, 等. 东北市售鸡肉中空肠弯曲杆菌的分离鉴定、分型及耐药性分析[J]. 食品与机械, 2018, 34(6): 46-49.
[16] ZAPKA C, LEFF J, HENLEY J, et al. Comparison of standard culture-based method to culture-independent method for evaluation of hygiene effects on the hand microbiome[J]. Mbio, 2017, 8(2): e00093-17.
[17] HUANG Ying, ZHANG Lu, TIU Laura, et al. Characterization of antibiotic resistance in commensal bacteria from an aquaculture ecosystem[J]. Frontiers in Microbiology, 2015, 6: 914-919.
[18] Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing[S]. 28th ed. Pennsylvania: Clinical and Laboratory Standards Institute, 2018: 30-97.
[19] 米瑞芳, 陈曦, 熊苏玥, 等. 传统自然发酵酸肉中细菌群落多样性与风味品质分析[J]. 食品科学, 2019, 40(2): 85-92.
[20] ROGNES T, FLOURI T, NICHOLS B, et al. VSEARCH: A versatile open source tool for metagenomics[J]. PeerJ, 2016, 4: e2584.
[21] HASS J, GEVERS D, EARL M, et al. Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons[J]. Genome research, 2011, 21(3): 494-504.
[22] WANG Qiong, GARRITY M, TIEDJE M, et al. Naive bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J]. Applied and Environmental Microbiology, 2007, 73(16): 5 261-5 267.
[23] 林奕岑, 徐帅, 倪学勤, 等. 利用Illumina MiSeq测序平台分析肉鸡盲肠微生物多样性[J]. 中国农业大学学报, 2016, 21(12): 65-73.
[24] 肖英平, 何祥祥, 戴宝玲, 等. 采样方法对冷鲜鸡表面细菌DNA提取及高通量测序结果的影响[J]. 食品科学, 2017, 38(24): 260-264.
[25] 温冬玲, 成淑君, 刘悦, 等. 高通量测序分析不同增菌温度下冷鲜鸡肉细菌的群落多样性[J]. 食品科学, 2018, 39(24): 156-161.
[26] LIU Dong, LIU Zeng-shan, HU Pan, et al. Characterization of a highly virulent and antimicrobial-resistant Acinetobacter baumannii strain isolated from diseased chicks in China [J]. Microbiology and Immunology, 2016, 60(8): 533-539.
[27] ANSARI F, KHATOON H. Multiple antibiotic resistance among gram negative bacteria isolated from poultry[J]. Indian Journal of Experimental Biology, 1994, 32(3): 211-212.
[28] KILONZO-NTHENGE A, ROTICH E, NAHASHON N. Evaluation of drug-resistant Enterobacteriaceae in retail poultry and beef[J]. Poultry Science, 2013, 92(4): 1 098-1 107.
[29] 李欣南, 韩镌竹, 宁宜宝. 鸡源奇异变形杆菌的分离鉴定及耐药性研究[J]. 黑龙江畜牧兽医, 2015(6): 165-167.
[30] ABU-ELALA N, ABDELSALAM M, MAROUF S, et al. Comparative analysis of virulence genes, antibiotic resistance, and gyrB based phylogeny of motile Aeromonas species isolates from Nile tilapia and domestic fowl[J]. Letters in Applied Microbiology, 2015, 61(5): 429-436.
[31] 刘星, 孟韩冰, 孔祥彬. 鸡肠毒综合征致病菌的自动生化鉴定及药敏分析[J]. 河南畜牧兽医: 综合版, 2008, 29(7): 7-9.
[32] 陈力力, 杨伊磊, 青文哲, 等. 鸡蛋壳表面细菌数量及种群多样性分析[J]. 现代食品科技, 2015, 31(10): 74-79.
[33] 戴宝玲, 肖英平, 孙凤来, 等. 家禽定点屠宰场不同屠宰区域空气的微生物结构[J]. 食品科学, 2018, 39(21): 219-223.
[34] SUGANTHI R, SHANMUGA-PRIYA T, SARANYA A, et al. Relationship between plasmid occurrence and antibiotic resistance in Myroides odoratimimus SKS05-GRD isolated from raw chicken meat[J]. World Journal of Microbiology Biotechnology, 2013, 29(6): 983-990.
[35] 牛天琦. 鸡粪中多重耐药细菌的分离鉴定及介导抗性基因水平转移元件的检测[D]. 新乡: 河南师范大学, 2015: 62-67.
[36] LI Gen, DU Xu-sheng, ZHOU De-fang, et al. Emergence of pathogenic and multiple-antibiotic-resistant Macrococcus caseolyticus in commercial broiler chickens[J]. Transboundary and Emerging Diseases, 2018, 65(6): 1 605-1 614.
[37] 陈佰义, 何礼贤, 胡必杰, 等. 中国鲍曼不动杆菌感染诊治与防控专家共识[J]. 中国医药科学, 2012, 2(8): 3-8.
[38] 万颖. 杭州地区不同来源气单胞菌的毒力基因分布与耐药性研究[D]. 杭州: 浙江大学, 2016: 1.
[39] 李仲兴, 张新华, 刘怀军. 漫游球菌的研究进展[J]. 国外医学: 临床生物化学与检验学分册, 2005, 26(9): 631-634.
[40] 杨虎, 向文良, 张弛, 等. 培养和非培养法分析冷藏鸡肉胴体中的细菌多样性[J]. 微生物学通报, 2010, 37(10): 1 451-1 456.
[41] OH J, BYRD A, PARK M, et al. Temporal stability of the human skin microbiome[J]. Cell, 2016, 165(4): 854-866.