Abstract
The main components of the alcohol extraction from peony stamen was analyzed by high performance liquid chromatography. H2O2 induced human umbilical vein endothelial cells (HUVEC) cells to establish a damage model to study the protective effect of peony flower extract. Results: the alcohol extraction from peony stamen mainly contain three monomers, namely rutin, quercetin and paeoniflorin, with contents of 44.25%, 15.50% and 17.00%, respectively. The alcohol extraction from peony stamen can reduce the content of MDA in cells and culture medium, increase the activities of SOD, GPX and GSH content in the calls. The alcohol extraction of peony stamen can improve the antioxidant capacity of HUVEC.
Publication Date
10-28-2019
First Page
97
Last Page
102
DOI
10.13652/j.issn.1003-5788.2019.10.019
Recommended Citation
Lei, LUO; Ningning, GUAN; Jinle, XIANG; and Wenxue, ZHU
(2019)
"Protective effects of alcohol extraction from peony stamen on H2O2-induced damage in HUVEC,"
Food and Machinery: Vol. 35:
Iss.
10, Article 19.
DOI: 10.13652/j.issn.1003-5788.2019.10.019
Available at:
https://www.ifoodmm.cn/journal/vol35/iss10/19
References
[1] 刘普, 卢宗元, 邓瑞雪, 等. 牡丹籽饼粕化学成分研究[J]. 中草药, 2013, 44(22): 3 105-3 108.
[2] 周远明, 郑国生, 马传利, 等. 牡丹雄蕊油的提取及化学成分研究[J]. 中国粮油学报, 2015, 30(3): 59-61.
[3] 李朝苹, 陈立勇, 阴英超, 等. 牡丹花蕊水提取液抗氧化功能研究[J]. 山东大学学报: 医学版, 2015, 53(11): 32-36.
[4] TABAS I, GARCACARDEA G, OWENS G K. Recent insights into the cellular biology of atherosclerosis[J]. Journal of Cell Biology, 2015, 209(1): 13-22.
[5] 刘珊, 李俐漫, 左浩江, 等. 粗壮女贞对氧化损伤血管内皮细胞的保护作用[J]. 现代预防医学, 2017, 44(2): 297-300.
[6] 杨兆颖. CEPO对PCI术后患者血清致人脐静脉内皮细胞损伤的保护作用及机制研究[D]. 长春: 吉林大学, 2017: 19-33.
[7] ZHANG Yang, LIU Jian, LUO Jiang-yun, et al. Upregulation of angiotensin(1-7)-mediated signaling preserves endothelial function through reducing oxidative stress in diabetes[J]. Antioxid Redox Signal, 2015, 23(11): 880-892.
[8] 杨艳娜, 金在顺, 杨光, 等. 芍药苷对H2O2诱导的H9C2心肌细胞损伤的保护作用[J]. 中华临床医师杂志: 电子版, 2017, 11(5): 762-765.
[9] 罗云梅, 付晓霞, 杨丹莉, 等. 淫羊藿苷对H2O2诱导的人脐静脉内皮细胞氧化损伤的保护作用及机制[J]. 中国新药与临床杂, 2016, 35(1): 40-45.
[10] WEST A P, BRODSKY I E, RAHNER C, et al. TLR signalling augments macrophage bactericidal activity through mitochondrial ROS[J]. Nature, 2011, 472(7 344): 476-480.
[11] 傅茂润, 刘峰, 赵海军, 等. 牡丹雄蕊的营养成分和抗氧化能力研究[J]. 中国食物与营养, 2011(5): 71-74.
[12] 闫慧娇, 赵伟, 耿岩玲, 等. 牡丹花化学成分研究[J]. 天然产物研究与开发, 2015, 27(12): 2 056-2 059.
[13] 孙泽飞. 牡丹花类黄酮成分及抗氧化能力分析[D]. 咸阳: 西北农林科技大学, 2015: 22-42.
[14] 王金艳, 任静, 陈世彬, 等. HPLC法同时测定达原饮中芒果苷等6种成分的含量[J]. 天津中医药大学学报, 2018, 37(1): 72-74.
[15] 刘竹青, 尹登科, 韩岚, 等. 桃红四物汤含药血清对过氧化氢损伤的人脐静脉内皮细胞的保护作用[J]. 中国中药杂志, 2013, 38(3): 402-406.
[16] 罗磊, 张冰洁, 马丽苹, 等. 金银花叶黄酮体外抗氧化能力及对H2O2诱导RAW264.7巨噬细胞损伤的保护作用[J]. 食品科学, 2018, 39(11): 139-145.
[17] 卞梦瑶, 方勇, 裴斐, 等. 生姜油树脂对H2O2引起RAW264.7巨噬细胞损伤的保护作用[J]. 食品科学, 2014, 35(1): 244-249.
[18] 黄汉昌, 姜招峰. 芦丁与人血清白蛋白相互作用的紫外可见光谱特性研究[J]. 天然产物研究与开发, 2011, 23(3): 476-481.
[19] 张蕾. 丹参素钠调节MAPKs通路抗动脉粥样硬化小鼠内皮细胞损伤的机制研究[D]. 广州: 南方医科大学, 2017: 23-35.
[20] 刘焕兰, 武夏林, 曲卫玲, 等. 琼玉口服液对衰老小鼠脑组织SOD活性及血清中SOD和MDA含量的影响[J]. 吉林中医药, 2013, 33(3): 278-280.
[21] SPINACI M, MUCCILLI V, BUCCI D, et al. Biological effects of polyphenol-rich extract and fractions from an oenological oak-derived tannin on in vitro swine sperm capacitation and fertilizing ability[J]. Theriogenology, 2019, 12: 82-89.
[22] 罗磊, 关宁宁, 杨永庆, 等. 牡丹花蕊黄酮对H2O2诱导RAW264.7巨噬细胞损伤的保护作用[J]. 食品科学, 2018, 39(21): 142-148.
[23] BEDOYA-RAMREZ D, CILLA A, CONTRERAS-CALDERN J, et al. Evaluation of the antioxidant capacity, furan compounds and cytoprotective-cytotoxic effects upon Caco-2 cells of commercial Colombian coffee[J]. Food Chemistry, 2017, 219(1): 364-372.
[24] 张冰洁. 金银花叶中黄酮类化合物的提取、纯化及抗氧化研究[D]. 洛阳: 河南科技大学, 2017: 20-35.
[25] 马静静, 郑彬, 张伟, 等. 土壤B[a]P叠加污染对蚯蚓体腔细胞SOD、POD和MDA的毒性效应[J]. 生态与农村环境学报, 2013, 29(6): 743-748.
[26] 周艳峰. 芦丁抑制H2O2诱导的人晶状体上皮细胞氧化损伤和凋亡及其机制研究[D]. 合肥: 安徽医科大学, 2016: 19-31.
[27] 宋献美, 钱丽丽, 梁瑞峰. 芍药苷对过氧化氢损伤的人脐静脉内皮细胞的保护作用[J]. 现代预防医学, 2015, 42(16): 2 914-2 916, 2 923.
[28] 王微, 张晔, 刘畅, 等. 芍药苷对模拟缺血再灌注后人脐静脉内皮细胞胆碱能抗炎通路及相关因子的影响[J]. 中华中医药杂志, 2016, 31(3): 1 106-1 108.
[29] SHOKOOHINIA Y, RASHIDI M, HOSSEINZADEH L, et al. Quercetin-3-O-β-d-glucopyranoside, a dietary flavonoid, protects PC12 cells from H2O2-induced cytotoxicity through inhibition of reactive oxygen species[J]. Food Chemistry, 2015, 167: 162-167.
[30] DAUBNEY J, BONNER P L, HARGREAVES A J, et al. Cardioprotective and cardiotoxic effects of quercetin and two of its in vivo metabolites on differentiated H9c2 cardiomyocytes[J]. Basic Clin Pharmacol Toxicol, 2015, 116(2): 96-109.
[31] 褚韦韦, 吴国水, 孙雪东. 槲皮素对肾细胞氧化损伤的保护作用及机制[J]. 浙江预防医学, 2015, 27(7): 677-681.