Study on the compound enzyme extraction process of Glehniae Radix and its physicochemical properties
Abstract
Response surface analysis were carried out to optimize the extraction process of polysaccharide from Glehniae Radix (GLP-E), and GLP-E was separated and purified with DEAE-52 ion exchanger. The monosaccharide composition, molecular weight, and the scavenging effect on DPPH free radicals were investigated. The results showed that the optimal extraction conditions of polysaccharides from G. Radix were as follows: the liquid-to-solid ratio was 30∶1 (mL/g), with the addition of 3% enzyme and the ratio of cellulase to papain at 3∶1, hydrolyzed at 70 ℃ for 3 h. Under the control of these conditions, the extraction rate of polysaccharides from G. Radix was （22.04±0.23）%. GLP-E was eluted sequentially with water and NaCl to obtain GLP-E1 and GLP-E2. GLP-E, GLP-E1 and GLP-E2 differed in monosaccharide composition and molecular weight, and both showed the scavenge ability of DPPH free radicals.
Publication Date
11-28-2019
First Page
191
Last Page
197
DOI
10.13652/j.issn.1003-5788.2019.11.037
Recommended Citation
Yongshuai, JING; Danshen, ZHANG; Ruijuan, ZHANG; Yu, HAN; Dongbo, LIU; Yuguang, ZHENG; and Lanfang, WU
(2019)
"Study on the compound enzyme extraction process of Glehniae Radix and its physicochemical properties,"
Food and Machinery: Vol. 35:
Iss.
11, Article 35.
DOI: 10.13652/j.issn.1003-5788.2019.11.037
Available at:
https://www.ifoodmm.cn/journal/vol35/iss11/35
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