Abstract
A simple and rapid method for the simultaneous determination of 14 kinds of glucocorticoids in grass carp was developed by the QuEChERS method coupled with ultra performance liquid chromatography quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Q/Orbitrap MS). The results indicated that the linearity of all the 14 glucocorticoids ranged from 2.0 ng/mL to 100.0 ng/mL with the correlation coefficients greater than 0.999. The LOD is in the range of 1.3~6.0 μg/kg and the LOQ is in the range of 4.5~20.0 μg/kg. The recoveries were in the range of 76.9% to 103.3%, with the precursor ions （n=6） in the range of 1.6%~6.3%. The method was simple, rapid, accurate, and suitable for the rapid detection of glucocorticoids in grass carp.
Publication Date
12-28-2019
First Page
100
Last Page
104,162
DOI
10.13652/j.issn.1003-5788.2019.12.018
Recommended Citation
Tao, LI; Xiao, YANG; Guifang, SUN; Yang, SONG; and Xinwang, ZHOU
(2019)
"Rapid determination of 14 glucocorticoids in grass carp using QuEChERS coupled with UPLC-Q/Orbitrap MS,"
Food and Machinery: Vol. 35:
Iss.
12, Article 18.
DOI: 10.13652/j.issn.1003-5788.2019.12.018
Available at:
https://www.ifoodmm.cn/journal/vol35/iss12/18
References
[1] WALKER D J, SPENCER K A. Glucocorticoid programming of neuroimmune function[J]. General and Comparative Endocrinology, 2018, 256: 80-88.
[2] 陈晨. 基于液质联用技术的糖皮质激素类药物体内含量测定的方法研究与应用[D]. 上海: 复旦大学, 2013: 4-6.
[3] 赵砚彬, 胡建英. 环境孕激素和糖皮质激素的生态毒理效应: 进展与展望[J]. 生态毒理学报, 2016, 11(2): 6-17.
[4] 周江, 黄艳美, 龚越飞, 等. 超高效液相色谱法测定美白、祛痘化妆品中4种禁用激素[J]. 化学分析计量, 2016, 25(2): 52-55.
[5] 张月辉, 董慧明. HPLC-DAD超高效液相柱法同时测定抗风湿类中成药中非法添加的10种糖皮质激素[J]. 山东化工, 2015, 44(4): 69-74.
[6] 邹红梅, 左舜宇, 黄东仁, 等. 高效液相色谱—串联质谱法同时测定水产品中雄激素和糖皮质激素残留[J]. 中国渔业质量与标准, 2016, 6(2): 45-50.
[7] 李晴, 罗辉泰, 黄晓兰, 等. 四级杆飞行时间串联质谱高通量筛查鱼肉中的药物残留[J]. 分析化学, 2014, 42(10): 1 478-1 485.
[8] 袁媛. 糖皮质激素多残留免疫研究与量子点在免疫分析中的应用[D]. 无锡: 江南大学, 2013: 3-4.
[9] 郭成方, 商少明, 刘俊康, 等. 大体积进样—非匀强电场扫集微乳毛细管电动色谱法测定化妆品中糖皮质激素[J]. 分析测试学报, 2016, 35(6): 686-691.
[10] MAKAROV A. Electrostatic axially harmonic orbital trapping: A high-performance technique of mass analysis[J]. Analytical Chemistry, 2000, 72(6): 1 156-1 162.
[11] MOL H G J, ZOMER P, KONGING M. Qualitative aspects and validation of a screening method for pesticides in vegetables and fruits based on chromatography coupled to full scan high resolution (orbitrap) mass spectrometry[J]. Analytical and Bioanalytical Chemistry, 2012, 403(10): 2 891-2 908.
[12] 杨璐齐, 李蓉, 高永清, 等. UPLC- Q- Orbitrap HRMS同时检测水产品中磺胺和喹诺酮类药物残留[J]. 食品与机械, 2017, 33(8): 38-43.
[13] 严华, 云环, 刘鑫, 等. UHPLC-LTQ Orbitrap MS测定鸡肉组织中5种糖皮质激素残留[J]. 分析测试学报, 2013, 32(8): 909-914.
[14] 刘芸, 丁涛, 廖雪晴, 等. 高效液相色谱—四极杆/静电场轨道阱高分辨率质谱法快速筛查中成药和保健食品中非法添加的42种化学药物[J]. 分析化学, 2016, 44(3): 423-429.