Abstract
In this study, the static adsorption behavior and desorption effects of 9 kinds of macroporous resins on natamycin were investigated. The static adsorption kinetics and isothermal adsorption process of the resins were discussed. The in-situ adsorption effects of the macroporous resins on natamycin were also studied in Streptomyces natalensis HW-2 fermentation broth. The results showed that the adsorption and desorption effects of HPD300 and HPD450 resins on natamycin were better, the adsorption rates were 83.47% and 96.14%, respectively. The desorption rates were 95.22% and 85.42%, respectively, and the adsorption capacity was 0.63 and 0.72 mg/g dry resins, respectively. HPD450 was more suitable for the adsorption and analysis of natamycin. According to the Langmuir and Freundlich parameters of isothermal adsorption of HPD450 resin, the adsorption of HPD450 resin conformed to Langmuir equation and was mainly monolayer adsorption. With the Addition of 4 g/30 mL HPD450 resin in 48 hours of fermentation to the fermentation broth for in-situ adsorption, the total yield of natamycin was increased by 66.27%.
Publication Date
12-28-2019
First Page
42
Last Page
46
DOI
10.13652/j.issn.1003-5788.2019.12.008
Recommended Citation
Yingying, ZHENG; Dahong, WANG; Peng, XU; Wenhao, SHEN; and Ying, ZHANG
(2019)
"In-situ adsorption kinetics of macroporous resin on natamycin,"
Food and Machinery: Vol. 35:
Iss.
12, Article 8.
DOI: 10.13652/j.issn.1003-5788.2019.12.008
Available at:
https://www.ifoodmm.cn/journal/vol35/iss12/8
References
[1] CROWLEY S, MAHONY J, SINDEREN D. Current perspectives on antifungal lactic acid bacteria as natural bio-preservatives[J]. Trends in Food Science & Technology, 2013, 33(2): 93-109.
[2] ILKNUR C, ARZU C M. Determination of antifungal effect of edible coatings containing Williopsis saturnus var. saturnus against Yeast and Mold growth on kashar cheese[J]. J Food Sci, 2019, 84(2): 311-318.
[3] MARTN J F, APARICIO J F. Enzymology of the polyenes pimaricin and candicidin biosynthesis[J]. Methods Enzymol, 2009, 459: 215-242.
[4] JUNEJA V K, DWIVEDI H P, YAN Xiang-he, et al. Novel natural food antimicrobials[J]. Annu Rev Food Sci Technol, 2012, 3: 381-403.
[5] ZENG Xin, MIAO Wen-yun, ZENG Hua-wei, et al. Production of natamycin by Streptomyces gilvosporeus Z28 through solid-state fermentation using agro-industrial residues[J]. Bioresource Technol, 2019, 273: 377-385.
[6] APARICIO J F, BARREALES E G, PAYERO T D, et al. Biotechnological production and application of the antibiotic pimaricin: Biosynthesis and its regulation[J]. Appl Microbiol Biotechnol, 2016, 100: 61-78.
[7] 张艳敏. 褐黄孢链霉菌生产纳他霉素的研究[M]. 济南: 齐鲁工业大学, 2016: 6-7.
[8] STARK D, VONSTPOCKAR U. In situ product removal (ISPR) in whole cell biotechnology during the last twenty years[J]. Advances in Biochemical Engineering, 2003, 80: 149-175.
[9] SUN Rui, FU Kusng, FU Yu-jie, et al. Preparative separation and enrichment of four taxoids from Taxus chinensis needles extracts by macroporous resin column chromatogra-phy[J]. Journal of Separation Science, 2015， 32(9): 1 284-1 293.
[10] 魏宝东, 林雨舒. 发酵液分离提取条件对纳他霉素提取效果的影响[J]. 食品科技, 2010, 35(4): 204-207.
[11] 郑喜, 金志华, 梅乐和, 等. 普那霉素发酵与吸附分离耦合过程动力学[J]. 化工学报, 2010, 61(3): 668-675.
[12] 杨冰, 许颖, 季君晖, 等. 琥珀酸发酵过程中的产物抑制特征及树脂吸附原位分离的研究[J]. 食品与发酵工业, 2011, 37(4): 12-16.
[13] 王大红, 原江锋. 大孔树脂对喹乐霉素的静态吸附研究[J]. 精细化工, 2012, 29(7): 670-672.
[14] 姜喜成, 秦培勇, 谭天伟. 大孔树脂对丹参酮ⅡA的静态吸附研究[J]. 北京化工大学学报: 自然科学版, 2009, 36(3): 79-82.
[15] 李园园, 邹少华, 李洪娟, 等. 梨多酚在大孔树脂上的吸附性能研究[J]. 山东化工, 2018, 47(2): 30-32, 34.
[16] 赵丽娟, 李倩, 李晨阳, 等. 天山堇菜秦皮乙素大孔树脂分离纯化工艺优化[J]. 食品与机械, 2019, 35(5): 178-184.
[17] LIU Yong-feng, LIU Jun-xi, CHEN Xiao-feng, et al. Preparative separation and purification of lycopene from tomato skins extracts by macroporous adsorption resins[J]. Food Chemistry, 2010, 123(4): 1 027-1 034.
[18] 徐浩, 吴兆亮, 殷昊, 等. 发酵吸附分离耦合生产乳酸链球菌素工艺[J]. 高分子材料科学与工程, 2010, 26(10): 155-158.
[19] 王卫, 李忠海, 黎继烈, 等. 赤霉素发酵与大孔树脂吸附过程耦合工艺优化[J]. 中国生物工程杂志, 2012, 32(11): 70-74.