Establishment of amoxicillin content detection method in milk by Surface Enhanced Raman Spectroscopy
Abstract
In this study, the silver colloid solution biosynthesized by Lilium casa blanca petals powders was used as the Surface-enhanced Raman Scattering (SERS) active substrate to establish a SERS detection method for amoxicillin content in milk. The effect of the concentration of silver colloid solution, the volume ratio of silver colloid solution and amoxicillin solution, and the addition of sodium chloride on SERS detection were investigated to optimize the detection conditions. The results showed that, under the conditions of the concentration of silver colloid solution of 106.04 μg/mL, with the volume ratio of silver colloid solution and amoxicillin solution of 4:1, and without adding sodium chloride, the SERS signal was the strongest. Under these conditions, the concentrations of amoxicillin solution had a good linear relationship with the SERS intensity at 1 055 cm-1 in the range of 1～1 000 μg/mL, with a minimum detectable concentration of 1 μg/mL. The recoveries of amoxicillin in the three spiked milks ranged from 84.51% to 93.62% with a relative standard deviation (RSD) of 5.16%. In conclusion, using this simple and convenient method, a certain concentration of amoxicillin in milk could be detected.
Publication Date
2-28-2019
First Page
87
Last Page
91
DOI
10.13652/j.issn.1003-5788.2019.02.017
Recommended Citation
Haimin, LI; Qi, LIANG; Weiping, CHEN; and Juan, DU
(2019)
"Establishment of amoxicillin content detection method in milk by Surface Enhanced Raman Spectroscopy,"
Food and Machinery: Vol. 35:
Iss.
2, Article 17.
DOI: 10.13652/j.issn.1003-5788.2019.02.017
Available at:
https://www.ifoodmm.cn/journal/vol35/iss2/17
References
[1] EID M A, YUSOF N A, FARUQ M, et al. Quantitative measurement of amoxicillin in ibuprofen tablets using UPLC[J]. Measurement, 2016, 93: 465-472.
[2] LEFEUVRE S, BOIS-MAUBLANC J, HOCQUELOUX L, et al. A simple ultra-high-performance liquid chromatogra-phy-high resolution mass spectrometry assay for the simultaneous quantification of 15 antibiotics in plasma[J]. Journal of Chromatography B, 2017, 1 065/1 066: 50-58.
[3] FREITAS A, BARBOSA J, RAMOS F. Determination of amoxicillin stability in chicken meat by liquid chromatogra-phy-tandem mass spectrometry[J]. Food Analytical Methods, 2012, 5(3): 471-479.
[4] WANG Bo, PANG Mao-da, XIE Xing, et al. Quantitative analysis of amoxicillin, amoxicillin major metabolites, and ampicillin in chicken tissues via ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry[J]. Food Analytical Methods, 2017, 10(10): 3 292-3 305.
[5] 陈瑞春, 贾海涛, 艾连峰, 等. 高效液相色谱—串联质谱法测定牛奶和奶粉中9种青霉素类药物残留量[J]. 食品科学, 2011, 32(18): 249-252.
[6] 樊玉霞, 赖克强, 黄轶群. 表面增强拉曼光谱技术在食品痕量化学危害检测中的应用[J]. 光谱学与光谱分析, 2014, 34(7): 1 859-1 864.
[7] 房晓倩, 彭彦昆, 王文秀, 等. 基于表面增强拉曼的鸡尾酒中苯甲酸钠和山梨酸钾的同时快速检测[J]. 光谱学与光谱分析, 2018, 38(9): 2 794-2 799.
[8] 李韦, 范贤光, 王昕, 等. 基于SERS的食品中乌洛托品快速检测系统设计[J]. 光谱学与光谱分析, 2017, 37(6): 1 778-1 783.
[9] 李言, 谢云飞, 钱和, 等. 表面增强拉曼光谱快速检测赤藓红[J]. 食品工业科技, 2013(11): 307-309.
[10] 许丽梅, 康靖, 曾勇明, 等. SERS技术应用于食品中罗丹明B的快速检测[J]. 食品工业科技, 2017, 38(24): 238-242.
[11] 陈思, 郭平, 骆鹏杰, 等. 拉曼光谱法快速检测硬糖中的诱惑红[J]. 食品与机械, 2016, 32(4): 76-79.
[12] 陈思, 郭平, 万建春, 等. 白酒中糖精钠添加剂表面增强拉曼光谱快速检测研究[J]. 光谱学与光谱分析, 2017, 37(5): 1 412-1 417.
[13] JI Wei, WANG Li, QIAN He, et al. Quantitative analysis of amoxicillin residues in foods by surface-enhancedraman spectroscopy[J]. Spectroscopy Letters, 2014, 47(6): 451-457.
[14] PENG Yi-jie, LIU Mu-hua, CHEN Xiong-fei, et al. Surface-enhanced Raman spectroscopy coupled with gold nanoparticles for rapid detection of amoxicillin residues in duck meat[J]. Spectroscopy Letters, 2017, 50(10): 579-584.
[15] 赵进辉, 李耀, 袁海超, 等. 鸭肉中环丙沙星残留的表面增强拉曼光谱测定[J]. 分析测试学报, 2017(5): 701-704.