Studies on the antioxidant and enzyme inhibitory activities of Galangal methanol extract and the correlation with the bioactive components

Authors

LI Jiao, College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, China
ZHANG Xiaofeng, College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, China
WANG Xiaokai, College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, China
WANG Wanyu, College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, China

Abstract

The Galangal was degreased and extracted by methanol to evaluate its polyphenols content and total flavonoids content, and the relationship of them with the antioxidant capacity and inhibitory activities against pancreatic lipase and α-glucosidase were studied. The polyphenols and total flavonoids were determined respectively by Folin-Ciocalteu assay and AlCl₃ colorimetric method. The antioxidant potential was estimated by DPPH method, ABTS method and FRAP method. Additionally, inhibitory effects against pancreatic lipase and α-glucosidase in vitro were also assessed. The results showed that the polyphenols and total flavonoids were 62.91 mg GAE/g DW and 13.12 mg QE/g DW, respectively. When concentration of Galangal methanol extract was near 50 mg/mL, the scavenging rate of DPPH reached 95.78% (IC₅₀=6.37 mg/mL) and ABTS reached 99.03% (IC₅₀=2.24 mg/mL). The FRAP value of the methanol extract was 428.92 μmol Fe²⁺/g DW. The results also showed that inhibitory activities against pancreatic lipase and α-glucosidase, expressed by IC₅₀, were 205.87 mg/mL and 1.32 mg/mL, respectively. In summary, the Galangal contained abundant in polyphenols and flavonoids, and had strong antioxidant and inhibitory activities against pancreatic lipase and α-glucosidase, and this inclined its prospective explorement of the hypolipidemic and hypoglycemic products.

Publication Date

2-28-2019

First Page

140

Last Page

144,184

DOI

10.13652/j.issn.1003-5788.2019.02.027

Recommended Citation

Jiao, LI; Xiaofeng, ZHANG; Xiaokai, WANG; and Wanyu, WANG (2019) "Studies on the antioxidant and enzyme inhibitory activities of Galangal methanol extract and the correlation with the bioactive components," Food and Machinery: Vol. 35: Iss. 2, Article 27.
DOI: 10.13652/j.issn.1003-5788.2019.02.027
Available at: https://www.ifoodmm.cn/journal/vol35/iss2/27

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